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41篇 您的检索式:作者名="Malzahn"
    题名 作者 年代 出处 被引量
1Robust Transcriptional Activation in Plants Using Multiplexed CRISPR-Act2.0 and mTALE-Act Systems显示文摘为内长的基因的柔韧的 transcriptional 激活的用户友好的工具高度在植物被要求。我们以前显示出那由撤销联系 CRISPR 的蛋白质组成的一个 dCas9-VP64 系统(dCas9 ) 9 与四辆双人脚踏车熔化了使活跃之物 VP16 (VP64 ) 能被用于的 transcriptional 的重复在植物的内长的基因的 transcriptional 激活。在这研究,我们在植物为提高的 transcriptional 激活开发了向量系统的第二代。我们为基于 dCas9 的 transcriptional 激活测试了多重策略,并且由 dCas9 和产出的修改指南 RNA 支架 gRNA2.0 (指明的 CRISPR-Act2.0 ) 发现那 VP64 的同时的招募更强壮的 transcriptional 激活比 dCas9-VP64 系统。而且,我们开发了复合抄写为在植物的多达四基因的同时的激活的像使活跃之物的受动器激活(mTALE 行为) 系统。我们的结果建议 mTALE 行为是比在测试的大多数情况中的 CRISPR-Act2.0 甚至更有效的。另外,我们用积极反馈环探索了织物特定的基因激活。有趣地,我们的学习比当由激活探针使不安时,其它可以引起目标基因 silencing 到 transcriptional 激活,和紧调整的基因的表明某些内长的基因是更顺从的。因此,这些新工具能被用来调查基因规章的网络和他们的控制机制。复合 CRISPR-Act2.0 和 mTALE 行为系统的汇编是两个基于优化并且克隆策略的 PCR 独立的金门和网关,它将在 dicots 和 monocots 便于 transcriptional 激活应用程序。Levi G. Lowder Jianping Zhou Yingxiao Zhang Aimee Malzahn Zhaohui Zhong Tzung-Fu Hsieh Daniel F. Voytas Yong Zhang Yiping Qi 2018Molecular Plant2018,11,2:19
2An integrated project management information system 显示文摘MALZAHN UWE SCHULZ RALF 1996Turku Centre for Computer Science Technical Report1996,6,33:2
3A CAG repeat polymorphism of KCNN3 predicts SK3 channel function and cognitive performance in schizophrenia显示文摘Grube S Gerchen MF Adamcio B Pardo LA Martin S Malzahn D 2011EMBO Mol Med2011,3,6:1
494 GHz TWT for Military Radar Applicatrion显示文摘Gerum W Lippert G Malzahn P 2001IEEE Trans on Electron Devices2001,48,1:1
5Biological and Prognostic significance of stratified epithelial cytokeratins in infiltrating ductal breast carcinomas显示文摘Malzahn K Mitze M Thoenes M 1998Virchows Arch1998,433,2:1
6Biological and prognosticsignificance of stratified epithelial cytokeratins in infiltrating ductalbreast carcinomas显示文摘Malzahn K Mitze M Thoenes M 1998Virchows Arch1998,433,2:1
7Decision-making inthe diagnosis and treatment of stroke-associated pneumonia显示文摘Harms H Hoffmann S Malzahn IJ 2012Journal of Neurology Neurosurgery &- Psychiatry2012,83,12:1
8Development of a clinical score (A2DS2) to predict pneumonia in acute ischemic stroke显示文摘Hoffmann S Malzahn U Hamas H 2012Stroke2012,43,10:1
9An approximate analytical approach to resampling averages显示文摘Malzahn Dorthe Opper Manfred 2004Journal of Machine Learning Research2004,4,6:1
10Elevated temperature microstructural stability of heavily cold-worded in situ composites显示文摘Malzahn Kampe J C Courtney T H 1986Scripta Materialia1986,20,:1
11Biological and prognostic significance of stratified epithelial cytokeratis in infil- trating ductal breast carcinomas 显示文摘MALZAHN K MITZE M THOENES M 1998Virchows Arch1998,433,2:1
12Biological and prognostic significance of stratified epithelial cytokeretins in infiltrating ductal breast carcinomas显示文摘Malzahn K Mitze M Thoenes M 1998Virchows Arch1998,433,2:1
13Development of a Clinical Score (A2DS2) to Predict Pneumonia in Acute Ischemic Stroke显示文摘Sarah Hoffmann Uwe Malzahn Hendrik Harms Hans-Christian Koennecke Klaus Berger Marianne Kalic Georg Walter Andreas Meisel Peter Ulrich Heuschmann 2012Stroke2012,,10:1
14Biological and prog- nostic significance of stratified epithelial cytokeratins in in- filtrating ductal breast carcinomas 显示文摘Malzahn K Mitze M Thoenes M 1998Virchows Arch1998,433,2:1
15Decision-making in the diagnosis and treatment of stroke-associated pneumonia 显示文摘Harms H Hoffmann S Malzahn U 2012J Neurol Neurosurg Psychiatry2012,83,12:1
16White matter lesion severity in mild acute isehemie stroke patients and functional outcome after 1 year显示文摘Leonards CO Ipsen N Malzahn U 2012Stroke2012,43,11:1
17Resilience of North Sea phytoplankton spring blooms dynamics:an analysis of long term data at Helgoland Roads显示文摘Wiltshire K H Malzahn A M Wirtz K 2008Limnology and Oceanography2008,53,:1
18Development of a clinical score (A2DS) to predict pneumonia in acute ischemic stroke显示文摘Hoffmann S Malzahn U Harms H 2012Stroke2012,43,10:1
19Task design and modification based on physical ability measurement显示文摘Rahimi M Malzahn D E 1984Human Factors1984,26,6:1
20Decision-mak- ing in the diagnosis and treatment of stroke-associated pneumonia显示文摘Harms H Hoffmann S Malzahn U 2012J Neurol Neurosurg Psychiatry2012,83,12:1
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