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| 1 | In vitro and in silico analysis of dual-function peptides derived from casein hydrolysate显示文摘There is no study on food-derived peptide with both anticoagulant and angiotensin I-converting enzyme inhibitory (ACEI) activities yet. In this work, the anticoagulant and ACEI activities of the casein hydrolysates released by pepsin digestion were evaluated for the first time to the best of our knowledge. Results indicated that the casein hydrolysate exhibited potent anticoagulant activity by prolonging the thrombin time (TT) and the activated partial thromboplastin time (APTT). Compared with control samples, at 10 mg/mL, the TT and APTT of casein hydrolysate were 186.0 % ± 6.6 % and 163.5 % ± 7.4 %, respectively. The casein hydrolysate also showed a strong ACEI activity with an IC50 value of 1.775 mg/mL. The components of the bioactive casein hydrolysate were analyzed by nanoscale liquid chromatography quadrupole time-of-flight tandem mass spectrometry (NanoLC-Q-TOF-MS/MS). Total of 115 peptides were identified, among which 34, 9, 55 and 17 peptides were derived from α_(s1-), α_(s2-), β-, and κ-casein, respectively. The results of PeptideRanker and PepSite 2 analysis showed that 6 peptides (FRQFYQL, NENLLRF, NPWDQVKR, PVVVPPFLQ, PVRGPFPIIV, and ARHPHPHLSF) have both ACEI and anticoagulant activities by binding to the active sites of ACE and thrombin. This study indicated that casein is a potential functional food supplement that can be used for medical purposes. | Maolin Tu Xinyu Qiao Cong Wang Hanxiong Liu Shuzhen Cheng Zhe Xu Ming Du | 2021 | Food Science and Human Wellness2021，10，1： | 4 |
| 2 | Bone morphogenetic proteins:Relationship between molecular structure and their osteogenic activity显示文摘Bone morphogenetic proteins(BMPs)are a family of potent,multifunctional growth factors belonging to transforming growth factor-(TGF-).They are highly conservative in structures.Over 20 members of BMPs with varying functions such as embryogenesis,skeletal formation,hematopoiesis and neurogenesis have been identified in human body.BMPs are unique growth factors that can induce the formation of bone tissue individually.BMPs can induce the differentiation of bone marrow mesenchymal stem cells into osteoblastic lineage and promote the proliferation of osteoblasts and chondrocytes.BMPs stimulate the target cells by specific membrane-bound receptors and signal transduced through mothers against decapentaplegic(Smads)and mitogen activated protein kinase(MAPK)pathways.It has been demonstrated that BMP-2,BMP-4,BMP-6,BMP-7,and BMP-9 play an important role in bone formation.This article focuses on the molecular characterization of BMPs family members,mechanism of osteogenesis promotion,related signal pathways of osteogenic function,relationships between structure and osteogenetic activity,and the interactions among family members at bone formation. | Jian Yang Pujie Shi Maolin Tu Yun Wang Meng Liu Fengjiao Fan Ming Du | 2014 | Food Science and Human Wellness2014，3，3： | 4 |
| 3 | Beneficial effects of high-pressure homogenization on the dispersion stability of aqueous hydrolysate from Mytilus edulis显示文摘Owing to the formation of aggregation and gelation during storage,certain proteins and peptides exhibit limited applications in aqueous protein food products.The purpose of this study was to investigate the influence of homogenization and xanthan gum addition on the dispersion stability of Mytilus edulis hydrolysate(PHM).High-pressure homogenization(HPH)at 360 and 40 bar in the first and second values,respectively,and adding xanthan gum at a concentration of 1 mg/mL showed significantly improvement on the stability of the PHM solution.PHM-xanthan gum solutions(PHMX)showed the highest polypeptide precipitation rate and turbidity retention rate compared with those of PHM.Moreover,the centrifugal precipitation rate of PHMX without HPH was higher than that of homogeneous PHMX.After HPH treatment at 400 bar,the percentage of smaller particles in PHM and PHMX was increased,the aqueous system became more uniform,and the fluorescence intensity reached its maximum.HPH pretreatment improved the polypeptide dispersion stability and turbidity retention rate of PHM and PHMX and reduced the fluorescence intensity.The interactions of xanthan gum and polypeptide render the network microstructure more uniform under the conditions of homogenization,thus improving the dispersion stability of PHMX solutions.Therefore,under the premise of adding xanthan gum,HPH can better enhance the dispersion stability of the polypeptide in PHM. | Fengjiao Mao Maolin Tu Fengjiao Fan Chao Wu Cuiping Yu Ming Du | 2020 | Food Science and Human Wellness2020，9，4： | 0 |
| 4 | A novel strain of Levilactobacillus brevis PDD-5 isolated from salty vegetables has beneficial effects on hyperuricemia through anti-inflammation and improvement of kidney damage显示文摘Hyperuricemia is a metabolic disorder caused by abnormal purine metabolism,resulting in abnormally high serum uric acid.In this study,a novel Levilactobacillus brevis PDD-5 isolated from salty vegetables was verified with the function of alleviating hyperuricemia.The relevant effects of L.brevis PDD-5 in lowering uric acid were analyzed by in vitro and in vivo experiments.The results showed that the L.brevis PDD-5 has(68.86±15.46)%of inosine uptake capacity and(95.75±3.30)%of guanosine uptake capacity in vitro.Oral administration of L.brevis PDD-5 to hyperuricemia rats reduced uric acid,creatinine,and urea nitrogen in serum,as well as decreased inosine and guanosine levels in the intestinal contents of rats.Analysis of relevant markers in the kidney by ELISA kits revealed that L.brevis PDD-5 alleviated oxidative stress and inflammation.Moreover,the gene expression of uric acid transporter 1(URAT1)and glucose transporter 9(GLUT9)was down-regulated,and the gene expression of organic anion transporter 1(OAT1)was up-regulated after treatment with L.brevis PDD-5.Western blot analysis showed that L.brevis PDD-5 alleviated hyperuricemia-induced kidney injury through the NLRP3 pathway.The se findings suggest that L.brevis PDD-5 can lower uric acid,repair kidney damage,and also has the potential to prevent uric acid nephropathy. | Jue Xu Maolin Tu Xiankang Fan Yuxing Guo Tao Zhang Xiaoqun Zeng Zhendong Cai Zhen Wu Daodong Pan | 2024 | Food Science and Human Wellness2024，13，2： | 0 |
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