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| 1 | Underexpression of LATS1 TSG in colorectal cancer is associated with promoter hypermethylation显示文摘AIM:To investigate large tumor suppressor 1 (LATS1 ) expression, promoter hypermethylation, and microsatellite instability in colorectal cancer (CRC).METHODS:RNA was isolated from tumor tissue of 142 CRC patients and 40 colon mucosal biopsies of healthy controls. After reverse transcription, quantitative polymerase chain reaction (PCR) was performed, and LATS1 expression was normalized to expression of the ACTB and RPL32 housekeeping genes. To analyze hypermethylation, genomic DNA was isolated from 44 tumor CRC biopsies, and methylation-specific PCR was performed. Microsatellite instability (MSI) status was checked with PCR using BAT26, BAT25, and BAT40 markers in the genomic DNA of 84 CRC patients, followed by denaturing gel electrophoresis. RESULTS:Decreased LATS1 expression was found in 127/142 (89.4%) CRC cases with the average ratio of the LATS1 level 10.33 ± 32.64 in CRC patients vs 32.85 ± 33.56 in healthy controls. The lowest expression was found in Dukes' B stage tumors and G1 (welldifferentiated) cells. Hypermethylation of the LATS1 promoter was present in 25/44 (57%) CRC cases analyzed. LATS1 promoter hypermethylation was strongly associated with decreased gene expression; methylated cases showed 162× lower expression of LATS1 than unmethylated cases. Although high-grade MSI (mutation in all three markers) was found in 14/84 (17%) cases and low-grade MSI (mutation in 1-2 markers) was found in 30/84 (36%) cases, we found no association with LATS1 expression. CONCLUSION:Decreased expression of LATS1 in CRC was associated with promoter hypermethylation, but not MSI status. Such reduced expression may promote progression of CRC. | Piotr M Wierzbicki Krystian Adrych Dorota Kartanowicz Marcin Stanislawowski Anna Kowalczyk Janusz Godlewski Iwona Skwierz-Bogdanska Krzysztof Celinski Tomasz Gach Jan Kulig Bartlomiej Korybalski Zbigniew Kmiec | 2013 | World Journal of Gastroenterology2013，19，27： | 8 |
| 2 | Breaking wheat yield barriers requires integrated efforts in developing countries显示文摘Most yield progress obtained through the so called 'Green Revolution', particularly in the irrigated areas of Asia, has reached a limit, and major resistance genes are quickly overcome by the appearance of new strains of disease causing organisms.New plant stresses due to a changing environment are difficult to breed for as quickly as the changes occur.There is consequently a continual need for new research programs and breeding strategies aimed at improving yield potential, abiotic stress tolerance and resistance to new, major pests and diseases.Recent advances in plant breeding encompass novel methods of expanding genetic variability and selecting for recombinants, including the development of synthetic hexaploid, hybrid and transgenic wheats.In addition, the use of molecular approaches such as quantitative trait locus(QTL) and association mapping may increase the possibility of directly selecting positive chromosomal regions linked with natural variation for grain yield and stress resistance.The present article reviews the potential contribution of these new approaches and tools to the improvement of wheat yield in farmer's fields, with a special emphasis on the Asian countries, which are major wheat producers, and contain the highest concentration of resource-poor wheat farmers. | Saeed Rauf Maria Zaharieva Marilyn L Warburton ZHANG Ping-zhi Abdullah M AL-Sadi Farghama Khalil Marcin Kozak Sultan A Tariq | 2015 | Journal of Integrative Agriculture2015，14，8： | 2 |
| 3 | Restoration of iodide uptake in dedifferentiated thyroid carcinoma: relationship to human Na^+/I^- symporter gene methyladon status 显示文摘 | VENKATARAMAN GM YATIN M MARCINE K | 1999 | J Clin Endocrinol Metab1999，84，7： | 1 |
| 4 | Comparison of LaC13 : Ce and NaI (TI) Scintillators in -ray Spectrometry 显示文摘 | Marcin B Marek M Maciej K | 2005 | Nuclear Instruments and Methods in Physics Research2005，，： | 1 |
| 5 | Stochastic stability in three-player games显示文摘 | Dominik K Jacek M Marcin Z | 2005 | Bulletin of Mathematical Biology2005，67，6： | 1 |
| 6 | Estimation and testing of the Hurst parameter using p-variation显示文摘 | MARCIN M JAKUB K JUSTYNA W | 2013 | Journal of Physics A:Mathematical and Theoretical2013，46，32： | 1 |
| 7 | Total synthesis of (±)-batrachotoxinin A 显示文摘 | Kurosu M Marcin L R Grinsteiner T J | 1998 | J Am Chem Soc1998，120，26： | 1 |
| 8 | Elastic properties of the annular ligament of the human stapes-AFM measurement显示文摘 | Monika K Zygmunt R Marcin M | 2015 | JARO2015，16，： | 1 |
| 9 | Enterococcus fae cium EK13 an enterocin A-producing strain with probiotic character and its effect in piglets显示文摘 | Strompfova V Marcin akova M Simonora M | 2006 | Anaerobe2006，12，56： | 1 |
| 10 | Mitsunobu reaction of n-alkyl and n-aeyl sulfonamides-an efficient route to protected amines 显示文摘 | Henry J R Marcin L R MeIntosh M C | 1989 | Tetrahedron Lett1989，30，： | 1 |
| 11 | Preparation and Characterization of Ag-lntercalated Copper Hexacyanoferrate Films on Electrodes 显示文摘 | Marcin A M Pawel J K | 1996 | Electroanalysis1996，8，2： | 1 |
| 12 | Application of phenotypic microarrays to environmental microbiology显示文摘 | Sharon Borglin Dominique Joyner Kristen M DeAngelis Jane Khudyakov Patrik D’haeseleer Marcin P Joachimiak Terry Hazen | 2011 | Current Opinion in Biotechnology2011，，： | 1 |
| 13 | High-performance liquid chromatography method for the simultaneous determination of thiamine hydrochloride, pyridoxine hydrochloride and cyanocobalamin in pharmaceutical formulations using coulometric electrochemical and ultraviolet detection显示文摘 | MARCIN L M ANNA L WOJCIECH C | 2005 | J Chromatogr: A2005，1094，： | 1 |
| 14 | Confidence estimation of GMDH neural networks and its application in fault detection systems显示文摘 | Jozef K Marcin M | 2008 | International Journal of Systems Science2008，39，8： | 1 |
| 15 | Comparison of LaCl3:Ce and NaI(TI)scintillators in γ-ray spectrometry显示文摘 | MARCIN B MAREK M MACIEJ K | 2005 | Nuclear Instruments and Methods in Physics Research A2005，537，： | 1 |
| 16 | Stromal-Derived Factor 1 and Thrombopoietin Regulate Distinct Aspects of Human Megakaryopoiesis显示文摘 | MARCIN M ANNA J JANINA R | 2000 | Blood2000，96，13： | 1 |
| 17 | Cataytic ignition of ionic liquid for propellant appli-cation显示文摘 | Shamshina Julia L Smiglak Marcin Drab David M | 2010 | Chemical Communication2010，46，： | 1 |
| 18 | The role of the im- mune system and cytokines involved in the pathogenesis of autoimmune thyroid disease(AITD)显示文摘 | Hanna M Marcin M Monika OM | 2014 | Endocrin Polska2014，65，2： | 1 |
| 19 | The conceptual model of an eco-industrial park based upon ecological relationships 显示文摘 | EWA L B MARCIN B ANDRZEJ M | 2009 | Journal of Cleaner Production2009，17，： | 1 |
| 20 | Elec- trostatic self-assembly of binary nanoparticle crys- tals with a diamond-like lattice 显示文摘 | Kakin A M Marcin F Paszewski M | 2006 | Science2006，312，： | 1 |
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