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| 1 | Transactivation of the TIEG1 confers growth inhibition of transforming growth factor-β-susceptible hepatocellular carcinoma cells显示文摘AIM:To investigate the role of transforming growth factor(TGF)-β-inducible early gene 1(TIEG1) in TGF-β-induced growth inhibition in hepatocellular carcinoma(HCC) cells.METHODS:Human hepatocyte and HCC cell lines with varied susceptibilities to TGF-β1 were tested by methylthiazoletetrazolium(MTT) assay.The expression changes of Smad2,Smad3,Smad4,Smad7,TIEG1 and TIEG2 gene following treatment with TGF-β1 in a TGF-β-sensitive hepatocyte cell line(MIHA),a TGF-β-sensitive hepatoma cell line(Hep3B) and two TGF-β-insensitive hepatoma cell lines(HepG2 and Bel7404) were examined.SiRNA targeting TIEG1 was transfected into Hep3B cells and the sensitivity of cells to TGF-β1 was examined.Overexpression of TIEG1 was induced by lentiviral-mediated transduction in TGF-β1-resistant hepatoma cell lines(Bel7404 and HepG2).MTT assay and 4',6-Diamidino-2-phenylindole staining were used to identify cell viability and apoptosis,respectively.The expression level of stathmin was measured by reverse transcriptase polymerase chain reaction and Western-blotting analysis,and stathmin promoter activity by TIEG1 was monitored by a luciferase reporter gene system.RESULTS:TIEG1 was significantly upregulated by TGF-β1 in the TGF-β1-sensitive HCC cell line,Hep3B,but not in the resistant cell lines.The suppression of TIEG1 by siRNAs decreased the sensitivity of Hep3B cells to TGF-β1,whereas the overexpression of TIEG1 mediated growth inhibition and apoptosis in TGF-β1-resistant HCC cell lines,which resembled those of TGF-β1-sensitive HCC cells treated with TGF-β1.Our data further suggested that stathmin was a direct target of TIEG1,as stathmin was signif icantly downregulated by TIEG1 overexpression,and stathmin promoter activity was inhibited by TIEG1 in a dose-dependent manner.CONCLUSION:Our data suggest that transactivation of TIEG1 conferred growth inhibition of TGF-β-susceptible human HCC cells. | Lei Jiang Yiu-Kay Lai Jin-Fang Zhang Chu-Yan Chan Gang Lu Marie CM Lin Ming-Liang He Ji-Cheng Li Hsiang-Fu Kung | 2012 | World Journal of Gastroenterology2012，18，17： | 13 |
| 2 | Photochemical smog in China:scientific challenges and implications for air-quality policies显示文摘INTRODUCTION Severe air-pollution events in many parts of China pose a major threat to health and ecosystems[1].China’s air pollution is concentrated to economically developed areas,such as Beijing–Tianjin–Hebei(BTH)and Pearl–River–Delta(PRD)[2,3].The situation has received considerable attention in | Mattias Hallquist John Munthe Min Hu Tao Wang Chak K Chan Jian Gao Johan Boman Song Guo Asa M Hallquist Johan Mellqvist Jana Moldanova Ravi K Pathak Jan BC Pettersson Hakan Pleijel David Simpson Marie Thynell | 2016 | National Science Review2016，3，4： | 8 |
| 3 | Molecular basis of von Hippel-Lindau syndrome in Chinese patients显示文摘背景 Von Hippel-Lindau ( VHL )症候群是预先安排的正染色体的主导的家庭癌症症候群影响个人到在用南部的汉语的 VHL 的基因基础大部分是的各种各样的 organs.The 的多重瘤 unknown.In 这研究,我们在九无关的南部的中国 families.Methods 描绘了 VHL 的变化光谱有 VHL 的临床的特征的九 probands ,二征兆并且八个无征状的家庭成员在这 study.Prenatal | SIU Wai-kwan MA Ronald Ching-wan LAM Ching-wan MAK Chloe Miu YUEN Yuet-ping LO Fai-man Ivan CHAN Kin-wan LAM Siu-fung LING Siu-cheung TONG Sui-fan SO Wing-yee CHOW Chun-chung TANG Mary Hoi-yin TAM wing-hung CHAN Albert Yan-wo | 2011 | Chinese Medical Journal2011，，2： | 6 |
| 4 | Orchestration of occludins, claudins, catenins and cadherins as players involved in maintenance of the blood-epididymal barrier in animals and humans显示文摘尽管精子在睾丸在精子发生期间被形成,阴囊的精子是不成熟的并且不能游泳或使肥沃。这些批评精子的功能在特定的钠环境被 theblood-epididymal 障碍创造的附睾被获得;附睾主细胞分泌的蛋白质把精子绑在成熟并且调整精子的 maturational 过程。在附睾, epithelialcell 房间相互作用被粘住的连接调停,为房间粘附必要,并且由紧密的连接,哪个形式 blood-epididymal 障碍。这些细胞的连接的规定被认为在在邻近的主细胞许可证之间的 epididymis.Tight 连接以内在精子成熟的过程代表一个关键决定因素在为精子成熟是必要的附睾的腔的特定的微型环境的形成。尽管我们成为了重要,在理解附睾功能和 blood-epididymal 障碍进行,用动物模型,人的附睾上有有限信息。如果我们将理解对人的附睾可归因的正常、病理学的条件工作,我们必须清楚地建立人的附睾的生理、细胞、分子的规定,描绘这些功能并且开发将使用 epididymalfunctions 到的临床的策略的 develop 工具改进不孕的处理。 | Daniel G. Cyr Mary Gregory Evemie Dube Julie Dufresne Peter T. K. Chan Louis Hermo | 2007 | Asian Journal of Andrology2007，9，4： | 3 |
| 5 | Artificial cold exposure induced stroke in renovascular hypertensive rats and its association with cold-inducible RNA binding protein mRNA expression in brain tissue and blood pressure显示文摘BACKGROUND: High incidence of stroke at interchange period of autumn and winter was demonstrated by epidemiological survey, and the specific causes should be further investigated. OBJECTIVE: To investigate the influence of artificial cold exposure on the incidence of stroke in renovascular hypertensive rats (RHR), and analyze the association with blood pressure and cold-inducible RNA binding protein (CIRP) mRNA expression in brain tissue. DESIGN: A completely randomized grouping design, a randomized control animal trial. SETTINGS: Lab of Neurology, the First Affiliated Hospital of Sun Yat-sen University; Department of Chemistry, Open laboratory of Chemical Biology, Institute of Molecular Technology for Drug Discovery and Synthesis, University of Hong Kong. MATERIALS: Male SD rats (n =460), weighing 80-100 g were obtained from Guangdong Province Health Animal Unit. A modified RXZ-300A intelligent artificial climate cabinet (Ningbo Jiangnan Instrument Co.,Ltd., China). METHODS: The experiment were processed in the Lab of Neurology, the First Affiliated Hospital of Sun Yat-sen University and the Open Laboratory of Chemical Biology, Institute of Molecular Technology for Drug Discovery and Synthesis, University of Hong Kong from October 2004 to November 2005. Rats (n = 400) were operated to establish 2-kidney 2-clip RHR model as described previously. The sham-operated rats (n =60) served as normotensive controls. Eight weeks later, 300 of RHR were randomly selected according to their systolic blood pressure (SBP) and divided into 3 sub-groups (n =100 per group): mild hypertensive group (SBP of 160-200 mm Hg), moderate hypertensive group (SBP of 200-220 mm Hg) and severe hypertensive group (SBP > 220 mm Hg). Each group was further divided into two groups (n =50) under ACE and non-ACE. Normal sham-operated SD rats (n =60), SBP < 140 mm Hg, were randomly divided into two groups: Sham-operated control group (n =30) under ACE and non-ACE. To establish the ACE and non-ACE treatment, rats were housed individually in artificial climate cabinet, and ACE was designed as three cycles of 12-hour light of 22 ℃ (7∶00-19∶00) and 12-hour dark of 4 ℃(19∶00-7∶00). The non-ACE group was kept at 22 ℃ throughout the experiment. MAIN OUTCOME MEASURES: Blood Pressure changes were measured and stroke symptom were observed; Expression of the CIRP were examined by reverse transcription-polymerase chain reaction. RESULTS: Finally 360 rats were involved in the analysis of results. ① Incidence of stroke: The incidence of stroke in 2k2c RHR was significantly higher after a three-day intermittent (12-hour) ACE (29.3%) as compared with that in non-ACE (17.3%) (P < 0.05). Furthermore, the severe hypertensive 2k2c RHR (BP > 220 mm Hg) was found to have much higher incidence of stroke (66%, 33/50) than the mild (8%, 4/50) and moderate (18%) hypertensive 2k2c RHR. ② CIRP mRNA in brain tissue: ACE treatment stimulated the mRNA expression of CIRP in non-stroke 2k2c RHR but not in stroke 2k2c RHR (P < 0.05). CONCLUSION: High blood pressure and low expression of CIRP are associated with ACE induced stroke. | Xiaoaena Shi Jianwen Lin Ying Peng Lally L.K. Chan Hsiang Fu Kung Marie C. Lin Ruxun Huang | 2007 | Neural Regeneration Research2007，2，8： | 2 |
| 6 | Lung function ref erence values in Chinese children and adolescents in Hong Kong显示文摘 | Mary SM Karlberg EM Chan KN | 2000 | Am J Respirate Care Med2000，16，2： | 1 |
| 7 | Circulating nitric oxide is suppressed in obstructive sleep apnea and is reversed by nasal continuous positive airway pressure显示文摘 | Mary SM Lam Bv Chan LY | 2000 | AmJ Respir Crit Care Med2000，162，6： | 1 |
| 8 | Embryonic XMab21l2 Expression Is Required for Gastrulation and Subsequent Neural Development显示文摘 | Gene T.C Lau Oscar G.W Wong Priscilla M.Y Chan Kin-Hang Kok Rebecca L.Y Wong King-Tung Chin Marie C.M Lin Hsiang-Fu Kung King L Chow | 2001 | Biochemical and Biophysical Research Communications2001，，5： | 1 |
| 9 | Smart homes: current features and future perspectives 显示文摘 | CHAN Marie CAMPO Eric | 2009 | Maturitas2009，，64： | 1 |
| 10 | A review of smart homes-Present state and future challen- ges显示文摘 | Marie Chan Daniel Esteve Christophe Escriba | 2005 | Computer Methods cine2005，91，1： | 1 |
| 11 | The use of folate-PEG-grafted-hybranched-PEI nonviral vector for the inhibition of glioma growth in the rat显示文摘 | Bing Liang Ming-Liang He Chu-yan Chan Yang-chao Chen Xiang-Ping Li Yi Li Dexian Zheng Marie C. Lin Hsiang-Fu Kung Xin-Tao Shuai Ying Peng | 2009 | Biomaterials2009，，23： | 1 |
| 12 | Effects of resistance training on cardiovascular health in non-obese active adolescents显示文摘AIM:To determine the benefits of a 10-wk resistance training programme on cardiovascular health in nonobese and active adolescents.METHODS:This is a pragmatic randomised controlled intervention.The study was carried out in a Hong Kong Government secondary school.Thirty-eight lean and active boys and girls were randomised to either the resistance training group or the control group.Students in the resistance training group received in-school 10-wk supervised resistance training twice per week,with each session lasting 70 min.Main outcome measures taken before and after training included brachial endothelial dependent flow-mediated dilation,body composition,fasting serum lipids,fasting glucose and insulin,high sensitive C-reactive protein,24-h ambulatory blood pressure and aerobic fitness.RESULTS:The only training related change was in endothelial dependent flow-mediated dilation which increased from 8.5%to 9.8%.A main effect of time and an interaction(P<0.005) indicated that this improvement was a result of the 10-wk resistance training.Main effects for time(P<0.05) in a number of anthropometric,metabolic and vascular variables were noted;however,there were no significant interactions indicating the change was more likely an outcome of normal growth and development as opposed to a training effect.CONCLUSION:Ten weeks of resistance training in school appears to have some vascular benefit in active,lean children. | Clare Chung-Wah Yu Alison Mary McManus Hung-Kwan So Ping Chook Chun-Ting Au Albert Martin Li Jack Tat-Chi Kam Raymond Chi-Hung So Christopher Wai-Kei Lam Iris Hiu-Shuen Chan Rita Yn-Tz Sung | 2016 | World Journal of Clinical Pediatrics2016，5，3： | 1 |
| 13 | Effect of WeiJia on carbon tetrachloride induced chronic liver injury显示文摘瞄准:为了在长期的肝损伤使用上学习 WeiJia 的效果,四氯化碳(CCl (4 )) 劝诱了肝损伤动物模型。方法: 称 180-220g 的 Wistar 老鼠随机被划分成三个组:正常控制组(组 A ) ,导致的肝损伤控制组织的 CCl (4 )( 组 B ) 和有 WeiJia 处理的正式就职组织的 CCl (4 )( 组 C ) 。每个组由 14 只老鼠组成了。肝损坏和纤维变性被皮下注射在 3 mL/kg 体重在齐墩果油与 40% CCl (4 ) 导致两次为为组 B 和 C 老鼠的八个星期的一个星期而齐墩果油被用于组 A 老鼠。从第三个星期开始,组 C 老鼠也在 1.25 microg/kg 体重的剂量收到了 WeiJia 的每日的腹膜内注射。动物在第五个星期被牺牲(4 男性, 3 女性) ,并且第八个星期(4 男性, 3 女性) 分别地。纤维变性的度被测量并且为肝纤维变性和功能包括透明质酸的血清学的标记(哈) ,类型 IV 骨胶原(CIV ) , gamma-glutamyl 转移酶(gamma-GT ) ,丙氨酸 aminotransferase (中高音) 和 aspartate aminotransferase (著名计算机生产厂商) 被决定。α平滑肌肌动朊(alpha-SMA ) 和原子抗原(PCNA ) 免疫组织化学也是的增殖的房间表现了。结果:当时, CCl (4 ) 正式就职在组 B 和组 C 老鼠导致了肝的损坏和纤维变性的开发与组 A 相比老鼠。在组 C 老鼠的 WeiJia 的处理能与组 B 老鼠相比显著地减少纤维变性条件。效果能在三星期处理以后被观察并且在八星期处理以后是更明显的。浆液哈,在八星期为组 C 老鼠的处理以后的 CIV ,中高音,著名计算机生产厂商和 gamma-GT 层次是 58+/-22 microg/L ( P<0.01 ), 57+/-21 microg/L ( P<0.01 ), 47+/-10 U/L ( P<0.01 ), 139+/-13 U/L ( P<0.05 )和 52+/-21 U/L ( P>0.05 )分别地,从导致的肝损伤控制组织的 CCl ( 4 )(组 B )类似于正常控制组(组 A ),却显著地不同。PCNA 的增加和在 alpha-SMA 表示水平的减少也被观察。结论:WeiJia 能改进肝功能和可能通过星形的房间活动的抑制的还原剂肝纤维变性。 | Pik-Yuen Cheung QiZhang Gan-Rong Bai Chi-Chun Fong Lin Shi Yue-Feng Shi Meng-SuYang DepartmentofBiology Ya-Ou Zhang Marie Chia-Mi Lin Bernard Chan Jay Chun Hsiang-Fu Kung | 2006 | World Journal of Gastroenterology2006，12，12： | 1 |
| 14 | Lung function reference values in Chinese children and adolescents in Hong Kong 显示文摘 | MARY S M KARLBERG E M CHAN K N | 2000 | Am J Respir Crit Care Med2000，16，2： | 1 |
| 15 | Lung function reference values in Chinese children and adolescents in Hong Kong,IL Prediction equations for plethysmographic lung volumes显示文摘 | Mary SM Karlberg EM Chan K | 2000 | Am J Respirat Crit Care Med2000，162，2： | 1 |
| 16 | The putative protein 6 of the severe acute respiratory syndrome-associated coronavirus: Expression and functional characterization显示文摘 | Hua Geng Yuet-Man Liu Wai-Sing Chan Anthony Wing-Ip Lo Deborah Mun-Yee Au Mary Miu-Yee Waye Yuan-Yuan Ho | 2005 | FEBS Letters2005，，30： | 1 |
| 17 | Dual Responsive, Stabilized Nanoparticles for Efficient In Vivo Plasmid Delivery显示文摘 | Hua Wei Lisa R. Volpatti Drew L. Sellers Don O. Maris Ian W. Andrews Ashton S. Hemphill Leslie W. Chan David S. H. Chu Philip J. Horner Suzie H. Pun | 2013 | Angew Chem Int Ed2013，，20： | 1 |
| 18 | Smart wearable systems: Current status and future challenges显示文摘 | Marie Chan Daniel Estève Jean-Yves Fourniols Christophe Escriba Eric Campo | 2012 | Artificial Intelligence In Medicine2012，，3： | 1 |
| 19 | Inflammatory tumour microenvironment is associated with superior survival in hepatocellular carcinoma patients显示文摘 | Valerie Chew Charlene Tow Marissa Teo Hing Lok Wong Jasmine Chan Adam Gehring Marie Loh Alexandre Bolze Richard Quek Victor K.M. Lee Kang Hoe Lee Jean-Pierre Abastado Han Chong Toh Alessandra Nardin | 2009 | Journal of Hepatology2009，，3： | 1 |
| 20 | 阿卡波糖心血管评估(ACE)试验依据与设计显示文摘心血管疾病(CVD)患者和糖耐量减低(IGT)者患2型糖尿病(T2DM)的风险较高。生活方式的调整或药物干预可以延缓T2DM的进展,但没有证据表明它们能够降低该人群的心血管风险。阿卡波糖是一种α-葡萄糖苷酶抑制剂,能够降低餐后血糖;其被证明能够使T2DM风险降低25%,并有可能降低无CVD的IGT受试者的心血管风险。阿卡波糖心血管评估(ACE)试验是一项随机、安慰剂对照、双盲、二级预防试验,受试者为7500例患有冠心病(定义为既往心肌梗死、不稳定型心绞痛或当前稳定的心绞痛)并伴有IGT且年龄I〉50岁的患者。以1:1的比例将患者随机分组,分别在标准CVD治疗药物基础上服用阿卡波糖50mg,每天3次或相匹配的安慰剂。排除标准包括糖尿病病史、过去3个月内发生过心血管事件、NYHAⅢ/Ⅳ级心力衰竭、重度肝病和重度肾功能损害。本研究在中国内地和中国香港的约150家医院内进行。主要复合心血管终点是至首次发生心血管死亡、非致死性心肌梗塞或非致死性卒中的时间。次要终点包括全因死亡和新发T2DM。ACE是一项对CVD进行二级预防的研究,用于评估冠心病患者和IGT者在标准心血管治疗的基础上加用阿卡波糖的效果。CVD的治疗与干预标准包括合理使用能降低心血管风险因素的药物,例如B肾上腺素能受体阻断剂、血管紧张素转换酶抑制剂、抗血小板药物和降血脂药物。在高血糖症患者中,血糖可能是另一个可调节的风险因素。流行病学证据表明,IGT会导致CVD发病率和死亡率上升,尽管其程度低于T2DM患者。餐后高血糖是餐后血糖代谢障碍的一个指标,它是心血管事件的一个独立的预测因子,因为负荷后血糖值与心血管事件的相关性比空腹血糖值更强,即使在非糖尿病受试者中也是这样。餐后代谢障碍影响动脉粥样硬化与CVD的机制包含多种因素。体内血糖的急性上升会诱导内皮功能障碍,可能是通过氧化应激引起。急性高血糖症也与血栓形成的激活以及炎性级联反应相关。用药物治疗来降低餐后高血糖会导致CVD的替代指标下降。在之前未用药物而被随机分配接受瑞格列奈(一种短效促泌剂,主要对餐后高血糖进行控制)或格列本脲(一种长效磺脲类药物)的患者中,使用瑞格列奈的患者C反应蛋白和白细胞介素-6的下降程度较大,颈动脉内一中膜厚度指标也是如此。阿卡波糖能够通过延缓碳水化合物的消化和吸收,降低餐后高血糖,改善内皮功能障碍,降低炎性指标(包括细胞核因子.xB活化及其核转位以及C反应蛋白),减少凝血因子(纤维蛋白原和凝血素),减少低密度脂蛋白氧化,降低颈动脉内一中膜厚度。相比那格列奈,阿卡波糖餐后血流量介导的血管扩张改善更显著。它也会放大二肽基肽酶4抑制剂阿格列汀的降血糖效应。IGT者中进行的降糖药物临床试验大都集中在预防T2DM上,而对心血管结局鲜有关注。STOP.NIDDM试验(1429名IGT受试者被随机分配阿卡波糖或安慰剂)显示,在平均3.3年的随访期后,阿卡波糖降低T2DM风险25%。后续分析也显示心肌梗死风险以及预先指定的任一心血管事件风险下降,但只有47名受试者发生了CVD。一项关于阿卡波糖的荟萃分析提示,阿卡波糖能显著降低T2DM患者的心肌梗死风险和心血管死亡、卒中、外周动脉疾病、心绞痛、心力衰竭和血管重建的风险。这些数据支持了该假设:使用阿卡波糖降低餐后高血糖能够消除IGT者的高CVD风险。目前为止唯一的有关降低餐后血糖药物的前瞻性CVD结局试验NAVIGATOR显示,那格列奈对心血管结局没有影响。ORIGIN试验(增大基础胰岛素用量以降低IGT者或早期T2DM患者的空腹血糖值)结果也显示对心血管结局没有影响。ACE试验的目的是确定阿卡波糖是否能够减少冠心病患者和IGT者中的心血管事件,其次要结局是T2DM的预防情况。 | Rury R Holman Mary A Bethel Juliana CN Chan Jean-Louis Chiasson Zoe Doran Junbo Ge Hertzel Gerstein Yong Huo John J McMurray Lars Ryden Winitha Liyanage Stefan Schroder Michal Tendera Michael J Theodorakis Jaakko Tuomilehto Wenying Yang Dayi Hu Changyu Pan 潘长玉 | 2016 | 国际内分泌代谢杂志2016，36，3： | 1 |
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