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| 1 | Vascularization and osteogenesis in ectopically implanted bone tissue-engineered constructs with endothelial and osteogenic differentiated adipose-derived stem cells显示文摘BACKGROUND A major problem in the healing of bone defects is insufficient or absent blood supply within the defect.To overcome this challenging problem,a plethora of approaches within bone tissue engineering have been developed recently.Bearing in mind that the interplay of various diffusible factors released by endothelial cells(ECs)and osteoblasts(OBs)have a pivotal role in bone growth and regeneration and that adjacent ECs and OBs also communicate directly through gap junctions,we set the focus on the simultaneous application of these cell types together with platelet-rich plasma(PRP)as a growth factor reservoir within ectopic bone tissue engineering constructs.AIM To vascularize and examine osteogenesis in bone tissue engineering constructs enriched with PRP and adipose-derived stem cells(ASCs)induced into ECs and OBs.METHODS ASCs isolated from adipose tissue,induced in vitro into ECs,OBs or just expanded were used for implant construction as followed:BPEO,endothelial and osteogenic differentiated ASCs with PRP and bone mineral matrix;BPUI,uninduced ASCs with PRP and bone mineral matrix;BC(control),only bone mineral matrix.At 1,2,4 and 8 wk after subcutaneous implantation in mice,implants were extracted and endothelial-related and bone-related gene expression were analyzed,while histological analyses were performed after 2 and 8 wk.RESULTS The percentage of vascularization was significantly higher in BC compared to BPUI and BPEO constructs 2 and 8 wk after implantation.BC had the lowest endothelial-related gene expression,weaker osteocalcin immunoexpression and Spp1 expression compared to BPUI and BPEO.Endothelial-related gene expression and osteocalcin immunoexpression were higher in BPUI compared to BC and BPEO.BPEO had a higher percentage of vascularization compared to BPUI and the highest CD31 immunoexpression among examined constructs.Except Vwf,endothelial-related gene expression in BPEO had a later onset and was upregulated and well-balanced during in vivo incubation that induced late onset of Spp1 expression and pronounced osteocalcin immunoexpression at 2 and 8 wk.Tissue regression was noticed in BPEO constructs after 8 wk.CONCLUSION Ectopically implanted BPEO constructs had a favorable impact on vascularization and osteogenesis,but tissue regression imposed the need for discovering a more optimal EC/OB ratio prior to considerations for clinical applications. | Jelena G Najdanović Vladimir J Cvetković Sanja T Stojanović MarijaĐVukelić-Nikolić Jelena MŽivković Stevo J Najman | 2021 | World Journal of Stem Cells2021，13，1： | 3 |
| 2 | An improved 16S rRNA based PCR method for the specific detection of Salmonella enterica 显示文摘 | Marija T Gorazd A | 2003 | International Journal of Food Microbiology2003，80，： | 1 |
| 3 | Determination of fosethyl aluminium by spectrophotometric method显示文摘 | DIMITRIJE S SVETLANA L MARIJA T | 2001 | Pesticidi2001，16，2： | 1 |
| 4 | An improved 16S rRNA based PCR method for the specific detection of Salmonella enterica 显示文摘 | Marija T Gorazd A | 2003 | International Journal of Food Microbiology2003，80，： | 1 |
| 5 | Epidemiology of pemphigus in Macedonia:A 15-year retrospective stud (1990-2004)显示文摘 | Marija T Dimitri S Suzana Kamberova | 2006 | International Journal of Dermatology2006，，： | 1 |
| 6 | Physicochemical characterization and in vivo skin performance of a novel alkyl polyglucoside emulsifier in natural cosmetic cream - bases 显示文摘 | MARKOVIC D B MARIJA T K LUKIC M | 2014 | Tenside Surfactants Detergents2014，54，2： | 1 |
| 7 | An improved 16S rRNA based PCR method for the specific detection of Salmonella enterica 显示文摘 | Marija T Gorazd A | 2003 | Inter J Food Microbiol2003，80，： | 1 |
| 8 | The antioxidant activity of conducting polymers in biomedical applications显示文摘 | Marija G N Jadranka T S | 2004 | Current Applied physics2004，，4： | 1 |
| 9 | An improved 16S rRNA based PCR method for the specific detection of Salmonella enterica显示文摘 | Marija T Gorazd A | 2003 | International Journal of Food Microbiology2003，80，1： | 1 |
| 10 | Toward Regional Transmission Provision and Its Pricing in New England显示文摘 | Marija D I Yong T Y | 1997 | Utilities Policy1997，6，3： | 1 |
| 11 | Toward regional transmission provision and itspricing in New England 显示文摘 | Marija D I Yong T Y | 1997 | Utilities Policy1997，6，3： | 1 |
| 12 | The antioxidant activity of con ducting polymers in biomedical applications 显示文摘 | MARIJA G N JADRANKA T S | 2004 | Current Applied physics2004，，4： | 1 |
| 13 | An improved 16a rRNA based PCR method for the specific detection of salmonella enterica显示文摘 | Marija T Gorazd A | 2003 | International Journal of Food Microbiology2003，80，： | 1 |
| 14 | Feedback Linearizing Control of Switched Reluctance Motors 显示文摘 | Marija I S Riccardo M David G T | 1987 | IEEE Transactions on Automatic Control1987，32，5： | 1 |
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