1篇
您的检索式：作者名="Marko Huttula"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Application of a novel porous tantalum implant in rabbit anterior lumbar spine fusion model:in vitro and in vivo experiments显示文摘Background Some porous materials have been developed to enhance biologic fusion of the implants to bone in spine fusion surgeries.However,there are several inherent limitations.In this study,a novel biomedical porous tantalum was applied to in vitro and in vivo experiments to test its biocompatibility and osteocompatibility.Methods Bone marrow-derived mesenchymal stem cells (BMSCs) were cultured on porous tantalum implant.Scanning electron microscope (SEM) and Cell Counting Kit-8 assay were used to evaluate the cell toxicity and biocompatibility.Twenty-four rabbits were performed discectomy only (control group),discectomy with autologous bone implanted (autograft group),and discectomy with porous tantalum implanted (tantalum group) at 3 levels:L3–L4,L4–L5,and L5–L6 in random order.All the 24 rabbits were randomly sacrificed at the different post-operative times (2,4,6,and 12 months;n = 6 at each time point).Histologic examination and micro-computed tomography scans were done to evaluate the fusion process.Comparison of fusion index scores between groups was analyzed using one-way analysis of variance.Other comparisons of numerical variables between groups were made by Student t test.Results All rabbits survived and recovered without any symptoms of nerve injury.Radiographic fusion index scores at 12 months post-operatively between autograft and tantalum groups showed no significant difference (2.89 ± 0.32 vs.2.83 ± 0.38, F= 244.60, P = 0.709).Cell Counting Kit-8 assay showed no significant difference of absorbance values between the leaching liquor group and control group (1.25 ± 0.06 vs.1.23 ± 0.04, t = –0.644, P = 0.545),which indicated the BMSC proliferation without toxicity.SEM images showed that these cells had irregular shapes with long spindles adhered to the surface of tantalum implant.No implant degradation,wear debris,or osteolysis was observed.Histologic results showed solid fusion in the porous tantalum and autologous bone implanted intervertebral spaces.Conclusion This novel porous tantalum implant showed a good biocompatibility and osteocompatibility,which could be a valid biomaterial for interbody fusion cages. | Ming Lu Song Xu Zi-Xiong Lei Dong LU Wei Cao Marko Huttula Chang-He Hou Shao-Hua Du Wei Chen Shuang-Wu Dai Hao-Miao Li Da-Di Jin | 2019 | Chinese Medical Journal2019，，1： | 7 |
您所在的机构暂未开通此部分的功能，请联系管理员