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1篇 您的检索式：作者名="Matthew Deaner"

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		题名	作者	年代	出处	被引量
	1	CRISPR-PIN:Modifying gene position in the nucleus via dCas9-mediated tethering显示文摘Spatial organization of DNA within the nucleus is important for controlling DNA replication and repair,genetic recombination,and gene expression.Here,we present CRISPR-PIN,a CRISPR/dCas9-based tool that allows control of gene Position in the Nucleus for the yeast Saccharomyces cerevisiae.This approach utilizes a cohesindockerin interaction between dCas9 and a perinuclear protein.In doing so,we demonstrate that a single gRNA can enable programmable interaction of nuclear DNA with the nuclear periphery.We demonstrate the utility of this approach for two applications:the controlled segregation of an acentric plasmid and the re-localization of five endogenous loci.In both cases,we obtain results on par with prior reports using traditional,more cumbersome genetic systems.Thus,CRISPR-PIN offers the opportunity for future studies of chromosome biology and gene localization.	Jyun-Liang Lin Holly Ekas Matthew Deaner Hal S.Alper	2019	Synthetic and Systems Biotechnology2019，4，2：	0

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