|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 鸡DMRT1基因CRISPR/Cas9载体构建及打靶效率的检测显示文摘DMRT1(Double sex and Mab-3 related transcription factor 1)是鸡性腺分化和发育的关键候选基因,其详细的功能和作用途径还未被阐明。为了解明DMRT1在鸡性腺分化和发育过程中的功能,研究首次利用在线sg RNA平台设计4条鸡DMRT1 sg RNA序列,构建了4个sg RNA表达载体:Guide-1、Guide-2、Guide-3和Guide-4。转染鸡DF-1成纤维细胞后,利用T7E1内切酶的方法检测4个表达载体的打靶效率。结果显示,成功构建了4条正确的鸡DMRT1 sg RNA表达载体,并且都能高效地在DF-1细胞中表达,其中Guide-4表达载体能有效地打靶DMRT1基因,打靶效率为24.82%。研究结果为进一步在鸡胚敲除DMRT1基因,研究其功能奠定了基础。 | 杨秀荣 邓继贤 赵德彪 Mike McGrew Sunil Nandi 蒋和生 Michael Clinton | 2017 | 中国家禽2017，39，2： | 9 |
| 2 | The meme machine review显示文摘 | McGrew W C | 2003 | Paleo Anthropology2003，，5： | 1 |
| 3 | Multicenter, ran- domized, double-blind, placebo-controlled studay on the effect of oral tolvaptan on left ventricular dilation and function in patients with heart failure and systolic dys- funetion显示文摘 | Udelson JE McGrew FA Flores E | 2007 | J Am Coil Cardiol2007，49，22： | 1 |
| 4 | Key establishment in large dynamic groups using one-way function trees显示文摘 | SHERMAN A McGREW D | 2003 | IEEE Trans on Software Engineering2003，29，5： | 1 |
| 5 | Long-term control of surgically treated glomus tympanium tumors显示文摘 | Forest JA Jackson CG McGrewe BM | 2001 | Otol Neurootol2001，22，2： | 1 |
| 6 | Oviduct specif- ic expression of two therapeutic proteins in transgenic hens显示文摘 | Lillico S G Sherman A McGrew M J | 2007 | Proe Natl Aead Sci2007，104，6： | 1 |
| 7 | NEXRAD:Next generation weather radar(WSR-88D)显示文摘 | W H Heiss D L McGrew D Sirmans | 1990 | Microwave Journal1990，33，01： | 1 |
| 8 | Rapid induction of pluripotency genes after exposure of human somatic ceils to mouse ES cell extracts 显示文摘 | Bru T Clarke C McGrew M J | 2008 | Exp Cell Res2008，314，14： | 1 |
| 9 | Quality attributes of yogurt withLactobacillus casei and various prebiotics显示文摘 | ARYANA K J MCGREW P | 2007 | LWT-Food Science andTechnology2007，40，10： | 1 |
| 10 | Oviduct-specificexpression of two therapeutic proteins in transgenic hens显示文摘 | Lillico S G Sherman A Mcgrew M J | 2007 | Proc Natl Acad Sci U S A2007，104，： | 1 |
| 11 | Free malignant cells in relation to recurrence of carcinoma of colon显示文摘 | McGrew EA LawsJF Cole WH | 1954 | JA MA1954，154，： | 1 |
| 12 | Key establishment in large dynamic groups using one-way function trees显示文摘 | Sherman A T McGrew D A | 2003 | IEEE Transactions on Software Engineering2003，29，5： | 1 |
| 13 | Key establishment in large dynamic groups 显示文摘 | Mcgrew Sherman | 1998 | Glenwood1998，，5： | 1 |
| 14 | Multicenter,randomized,double-blind,placebo-controlled study on the effect of oral tolvaptan on left ventricular dilation and function in patients with heart failure and systolic dysfunction显示文摘 | Udelson JE Mcgrew FA FLORES E | 2007 | Journal of the American College of Cardiology2007，49，22： | 1 |
| 15 | Expression of trimeric CD40 ligand in Pichia pastoris:use of a rapid method to detect high-level expression transformants显示文摘 | MCGREW JT LEISKE D DELL B | 1997 | Gene1997，187，2： | 1 |
| 16 | TGF-beta 1 causes airway fibrosis and increased collagen I and 3 mRNA in mice显示文摘 | Kenyon NJ Ward RW McGrew G | 2003 | Thorax2003，58，9： | 1 |
| 17 | Efficient production of germline transgenic chickens using lentiviral vectors 显示文摘 | McGrew M J Sherman A Ellard F M | 2004 | European Molecular Biology Organization2004，5，7： | 1 |
| 18 | CHC theory and the human cognitive abilities project:Standing on the shoulders of the giants of psychometric intelligence research显示文摘 | Mcgrew K S | 2009 | Intelligence2009，37，1： | 1 |
| 19 | Multicenter, randomized, double-blind, placebo-controlled study on the effect of oral tolvaptan on left ventricular dilation and function in patients with heart failure and systolic dysfunction显示文摘 | Udelson JE McGrew FA Flores E | 2007 | J Am Coil Cardiol2007，49，22： | 1 |
| 20 | Impact of treating sleep apnea in a child with autism spectrum disorder显示文摘 | Malow B A McGrew S G Harvey M | 2006 | Pediatr Neurol2006，34，4： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员