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| 1 | Discriminating mild from critical COVID-19 by innate and adaptive immune single-cell profiling of bronchoalveolar lavages显示文摘How the innate and adaptive host immune system miscommunicate to worsen COVID-19 immunopathology has not been fully elucidated.Here,we perform single-cell deep-immune profiling of bronchoalveolar lavage(BAL)samples from 5 patients with mild and 26 with critical COVID-19 in comparison to BALs from non-COVID-19 pneumonia and normal lung.We use pseudotime inference to build T-cell and monocyte-to-macrophage trajectories and model gene expression changes along them.In mild COVID-19,CD8^(+)resident-memory(TRM)and CD4^(+)T-helper-17(T_(H17))cells undergo active(presumably antigen-driven)expansion towards the end of the trajectory,and are characterized by good effector functions,while in critical COVID-19 they remain more naïve.Vice versa,CD4^(+)T-cells with T-helper-1 characteristics(TH1-like)and CD8^(+)T-cells expressing exhaustion markers(T_(EX)-like)are enriched halfway their trajectories in mild COVID-19,where they also exhibit good effector functions,while in critical COVID-19 they show evidence of inflammation-associated stress at the end of their trajectories.Monocyte-to-macrophage trajectories show that chronic hyperinflammatory monocytes are enriched in critical COVID-19,while alveolar macrophages,otherwise characterized by anti-inflammatory and antigen-presenting characteristics,are depleted.In critical COVID-19,monocytes contribute to an ATP-purinergic signaling-inflammasome footprint that could enable COVID-19 associated fibrosis and worsen disease-severity.Finally,viral RNA-tracking reveals infected lung epithelial cells,and a significant proportion of neutrophils and macrophages that are involved in viral clearance. | Els Wauters Pierre Van Mol Abhishek Dinkarnath Garg Sander Jansen Yannick Van Herck Lore Vanderbeke Ayse Bassez Bram Boeckx Bert Malengier-Devlies Anna Timmerman Thomas Van Brussel Tina Van Buyten Rogier Schepers Elisabeth Heylen Dieter Dauwe Christophe Dooms Jan Gunst Greet Hermans Philippe Meersseman Dries Testelmans Jonas Yserbyt Sabine Tejpar Walter De Wever Patrick Matthys CONTAGIOUS collaborators Johan Neyts Joost Wauters Junbin Qian Diether Lambrechts | 2021 | Cell Research2021，31，3： | 11 |
| 2 | Galactomannan in bronchoalveolar lavage fluid: a tool for diagnosing aspergillo- sis in intensive care unit patients 显示文摘 | Meersseman W Lagrou K Maertens J | 2008 | Am J Respir Crit Care Med2008，177，1： | 1 |
| 3 | Galactomannan in bronchoalveolar lavage fluid: a tool for diagnosing aspergillosis in in- tensive care unit patients 显示文摘 | Meersseman W Lagrou K Maertens J | 2008 | Am J Respir Crit Care Med2008，177，1： | 1 |
| 4 | Invasive aspergillosis in critically ill patients without malignancy 显示文摘 | Meersseman W Vandecasteele S J Wilmer A | 2004 | Am J Respir Crit Care Med2004，170，6： | 1 |
| 5 | Interaction between valproate and meropenem: a retrospective study显示文摘 | Spriet I Goyens J Meersseman W | 2007 | Ann Pharmacother2007，41，7： | 1 |
| 6 | Pregnancy during nitisinone treatment for tyrosinaemia type I: first human experience显示文摘 | Vanclooster A Devlieger R Meersseman W | 2012 | JIMD Rep2012，5，1： | 1 |
| 7 | Reduced cortisolmetabolism during critical illness显示文摘 | Boonen E Vervenne H Meersseman P | 2013 | N Engl J Med2013，368，16： | 1 |
| 8 | Invasive as-pergillosis in the intensive care unit显示文摘 | Meersseman W Lagrous K Maertens J | 2007 | Clin Infect Dis2007，45，2： | 1 |
| 9 | Reduced nocturnalACTH-driven cortisol secretion during critical illness显示文摘 | Boonen E Meersseman P Vervenne H | 2014 | Am JPhysiol Endocrinol Metab2014，306，8： | 1 |
| 10 | Invasive aspergillosis in the intensive care unit显示文摘 | Meersseman W Lagrou K Maertens J | 2007 | Clin Infect Dis2007，45，2： | 1 |
| 11 | Invasive aspergillosis in critically ill patients without malignancy显示文摘 | Meersseman W Vandecasteele SJ Wilmer A | | 0，，： | 1 |
| 12 | Impact of in- tensive insulin therapy on neuromuscular complications and ventilator dependency in the medical intensive care unit显示文摘 | Hermans G Wilmer A Meersseman W | 2007 | Am J Respir Crit Care Med2007，175，5： | 1 |
| 13 | Invasive asppergillosis in critically ill patients without malignancy显示文摘 | Meersseman W Vandeeasteele SJ Wilmer A | 2004 | Am J Respir Crit Care Med2004，170，： | 1 |
| 14 | Invasive aspergillosis in the intensive care unit显示文摘 | Meersseman W Lagrous K Maertens J | 2007 | Clin Infect Dis2007，45，： | 1 |
| 15 | Galactomannan in bronehoalveolar lavage fluid : a tool for diagnosing aspergillosis in intensive care unit patients 显示文摘 | Meersseman W Lagrou K Maertens J | 2008 | Am J Respir Crit Care Med2008，177，： | 1 |
| 16 | High-throughput MALDITOF discovery of genomic sequence polymorphisms 显示文摘 | Stanssens P Zabeau M Meersseman G | 2004 | Genome Res2004，14，： | 1 |
| 17 | Reduced cortisol metabolism during critical illness显示文摘 | Boonen E Vervenne H Meersseman P | 2013 | N Engl J Med2013，368，16： | 1 |
| 18 | Reduced cortisol metabolism during critical illness 显示文摘 | Boonen E Vervenne H Meersseman P | 2013 | N Engl J Med2013，368，16： | 1 |
| 19 | Galaetomanna- nin bronchoalveolar lavagefluid:a tool for diagnosing uspergil- losis in intensive care unit patients显示文摘 | Meersseman W Lagrou K Maertens J | 2008 | Am J Respir Grit Care Med2008，177，1： | 1 |
| 20 | Rapid and direct quantification of viable Candida species in whole blood by use of immunomagnetic separation and solid-phase cytometry 显示文摘 | VANHEE L M MEERSSEMAN W LAGROU K | 2010 | J Clin M icrobiol2010，48，4： | 1 |
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