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您的检索式：作者名="Meijun Yin"
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| 1 | Potential transcriptional regulatory regions exist upstream of the human ezrin gene promoter in esophageal carcinoma cells显示文摘我们以前证明这个区域 ? 人的 ezrin 基因(VIL2 ) 的 87/+134 在人的食道的癌 EC109 房间,和一个进一步在上游的区域展出了倡导者活动 ?1324/? 890 断然调整的抄写。在这研究,识别 transcriptional VIL2 倡导者在上游的规章的区域,我们克隆 VIL2 ?1541/? 包含 ? 1324/ 的 706 片断 ? 890,并且调查了它的 transcriptional 经由酶试金在的规章的性质短暂地 transfected 房间。在 EC109 房间,它被发现那 VIL2 ?1541/? 706 项拥有的倡导者和 enhancer 活动。我们也局部性的 transcriptional 由熔化 VIL2 的 5 删除或 3 删除片断的规章的区域 ?1541/? 706 到一个酶记者。我们发现有三积极并且一否定 transcriptional 在 VIL2 以内的规章的区域 ?1541/? 706 在 EC109 房间。当这些区域独立被定位时没有倡导者的酶基因在上游,或定位指导酶基因的 VIL2 倡导者或 SV40 倡导者在上游,仅仅 VIL2 ?1297/? 1186 展出了可观的倡导者和 enhancer 活动,它是比那些低的 ?1541/? 706。另外, Sp1 的短暂表示增加了 ezrin 表示和 VIL2 的 transcriptional 激活 ?1297/? 1186。尽管在删除实验展出显著地积极或否定的 transcriptional 规定,另外的三个区域显示出一条更弱或不在的规定。这些数据建议 VIL2 倡导者在上游的超过一个区域参予了 VIL2 抄写,和 VIL2 ?1297/? 1186,可能作为关键 transcriptional 规章的区域,在有另外的潜在的规章的区域的同伴的调整 VIL2 抄写。 | Shuying Gao Yanpeng Dai Meijun Yin Jing Ye Gang Li Jie Yu | 2011 | Acta Biochimica et Biophysica Sinica2011，43，6： | 4 |
| 2 | Heterogenous glucose-stimulated insulin secretion at single islet level显示文摘Insulin secretion by pancreatic islets plays a vital role in regulating blood glucose levels.Nevertheless,the mech-anism responsible for this dynamic insulin secretion has not been completely understood,particularly at the single islet level.In this study,we have successfully developed an easy microfluidic platform that allows for the exploration of dynamic glucose-stimulated insulin secretion(GSIS)at the single islet level.With the utilization of this platform,we evaluated dynamic GSIS from single islets isolated from both normal and diabetic rats.Our results demonstrate that islets can be categorized into three types based on their dynamic GSIS:Type Ⅰ exhibits a biphasic GSIS profile with a fast first phase and flat second phase;Type Ⅱ also has a biphasic GSIS profile with a fast first phase but a slow increased second phase;Type Ⅲ displays only a slowly increased second phase and lacks a fast first phase.RNA sequencing analysis demonstrated that the cell type and exocytosis-specific genes are consistent with the proportion of cells and insulin release kinetics among the three types of islets,respectively.Moreover,our findings suggest that high expression of Atp5pb is anti-correlated with the first phase of insulin secretion.Furthermore,we revealed that diabetic islets exhibit only the type Ⅰ GSIS response,indicating a deliberate impairment of the second phase of insulin secretion.Together,this device serves as a crucial tool in the research field of islets and diabetes,allowing researchers to investigate islet functional heterogeneity and identity at the single islet level. | Jiaxiang Yin Hao Meng Haopeng Lin Meijun Mo Jingfang Lin Jingyi Chen Lihua Chen Xiaojun Xu Zonghong Li Wei Ji Tao Xu Huisheng Liu | 2023 | Engineered Regeneration2023，4，4： | 0 |
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