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| 1 | Degradation of sodium an thraquin-one sulphonate by free and immobilized bacterial cultures显示文摘 | Meriem B Nevenka A Cesar P | 1994 | Appl Microbiol Biotechnol1994，41，1： | 1 |
| 2 | Degradation of sodium anthraquinone sulphonate by free and immobilized bacterial cultures显示文摘 | MERIEM B NEVENKA A CESAR P | 1994 | Appl MicrobiolBiotechnol1994，41，1： | 1 |
| 3 | The interference between mefformin treatment and TSH level in euthyroid type 2 diabetic patients 显示文摘 | Soumaya K Rym B Meriem A | 2013 | Med Chem Res2013，22，3： | 1 |
| 4 | Impact of water deficit intensity on durum wheat seminal roots显示文摘 | Ahmed A Mohamed S Meriem K H | 2005 | Comptes Rendus Biologies2005，328，： | 1 |
| 5 | Conditional dicer gene deletion in the postnatal myocardium provokes spontaneous cardiac remodeling显示文摘 | Paula A C M Meriem B Monika G | 2008 | Circulation2008，118，15： | 1 |
| 6 | Degradation of sodium and thraquinone sulphonate by free and immobilized bacterial cultures显示文摘 | Meriem B Nevenka A Cesar P | 1994 | Appl Microbiol Biotechnol1994，41，1： | 1 |
| 7 | Glucolipotoxicity of the pancreatic beta cell显示文摘 | Vincent P Julie A Meriem S | | 0，，： | 1 |
| 8 | Validity of the depressive dimension extracted from principal component analysis of the PANSS in drug-- free patients with schizophrenia 显示文摘 | Meriem EY Omar B Mohamed A | 2002 | Schizophr Res2002，56，12： | 1 |
| 9 | Profile of antimicrobial resistance of agents causing urinary tract infections in children 显示文摘 | Ghedira Besbes L Messaoudi A Ben Meriem C | 2004 | Tunis Med2004，82，3： | 1 |
| 10 | Extracellular vesicles from oviductal and uterine fluids supplementation in sequential in vitro culture improves bovine embryo quality显示文摘Background:In vitro production of bovine embryos is a well-established technology,but the in vitro culture(IVC)system still warrants improvements,especially regarding embryo quality.This study aimed to evaluate the effect of extracellular vesicles(EVs)isolated from oviductal(OF)and uterine fluid(UF)in sequential IVC on the development and quality of bovine embryos.Zygotes were cultured in SOF supplemented with either BSA or EVs-depleted fetal calf serum(dFCS)in the presence(BSA-EV and dFCS-EV)or absence of EVs from OF(D1 to D4)and UF(D5 to D8),mimicking in vivo conditions.EVs from oviducts(early luteal phase)and uterine horns(mid-luteal phase)from slaughtered heifers were isolated by size exclusion chromatography.Blastocyst rate was recorded on days 7-8 and their quality was assessed based on lipid contents,mitochondrial activity and total cell numbers,as well as survival rate after vitrification.Relative mRNA abundance for lipid metabolism-related transcripts and levels of phosphorylated hormonesensitive lipase(pHSL)proteins were also determined.Additionally,the expression levels of 383 miRNA in OF-and UF-EVs were assessed by qRT-PCR.Results:Blastocyst yield was lower(P<0.05)in BSA treatments compared with dFCS treatments.Survival rates after vitrification/warming were improved in dFCS-EVs(P<0.05).EVs increased(P<0.05)blastocysts total cell number in dFCS-EV and BSA-EV compared with respective controls(dFCS and BSA),while lipid content was decreased in dFCSEV(P<0.05)and mitochondrial activity did not change(P>0.05).Lipid metabolism transcripts were affected by EVs and showed interaction with type of protein source in medium(PPARGC1B,LDLR,CD36,FASN and PNPLA2,P<0.05).Levels of pHSL were lower in dFCS(P<0.05).Twenty miRNA were differentially expressed between OF-and UF-EVs and only bta-miR-148b was increased in OF-EVs(P<0.05).Conclusions:Mimicking physiological conditions using EVs from OF and UF in sequential IVC does not affect embryo development but improves blastocyst quality regarding survival rate after vitrification/warming,total cell number,lipid content,and relative changes in expression of lipid metabolism transcripts and lipase activation.Finally,EVs miRNA contents may contribute to the observed effects. | Cláudia Lima Verde Leal Karina Canón-Beltrán Yulia N.Cajas Meriem Hamdi Aracelli Yaryes María Gemma Millán de la Blanca Paula Beltrán-Brena Rosane Mazzarella Juliano Coelho da Silveira Alfonso Gutiérrez-Adán Encina M González Dimitrios Rizos | 2023 | Journal of Animal Science and Biotechnology2023，14，1： | 0 |
| 11 | Molecular identification of the potentially forensically relevant cluster flies Pollenia rudis (Fabricius) and Pollenia vagabunda (Meigen) (Diptera: Polleniidae) — non-recorded species in Algeria显示文摘Cluster flies are represented by the genus Pollenia Robineau-Desvoidy,1830 of the family Polleniidae Brauer and Bergenstamm,1889.Their larvae are known to be internal parasites or predators of earthworms.Herein,we report for the first time the occurrence of the cluster flies Pollenia rudis Fabricius,1794 and Pollenia vagabunda(Meigen,1826)(Diptera:Polleniidae)on carcasses in Algeria and identify them through DNA barcoding.A region of the mitochondrial cytochrome c oxidase I gene(COI)was amplified and sequenced.Genetic distances were determined.A phylogenetic tree was constructed with the maximum parsimony method using 10000 bootstrap replicates.A total number of 157 adults of P.rudis were collected together with 325 adults of Pollenia vagabunda.The occurrence of Pollenia on animal carcasses does not seem to be correlated with a particular stage of decomposition.All the sequences were correctly identified using the BLASTn tool from the GenBank database and the BOLD identification engine.Intra-and interspecific sequence divergence values were less than 1%and greater than 3%,respectively.COI barcodes obtained from this study were robust enough to identify and distinguish unambiguously between P.rudis and P.vagabunda.In the tree-based analysis,the cluster flies were all assigned to their respective species separately from each other confirming the morphological identification.These results provide DNA barcodes that contribute to the growth of reference databases and allow fast and accurate identification. | Meriem Taleb Ghania Tail Halide Nihal Açıkgöz | 2022 | Forensic Sciences Research2022，7，1： | 0 |
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