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| 1 | Tenascin C upregulates interleukin-6 expression in human cardiac myofibroblasts via toll-like receptor 4显示文摘AIM:To investigate the effect of Tenascin C(TNC)on the expression of pro-inflammatory cytokines and matrixmetalloproteinases in human cardiac myofibroblasts(CMF).METHODS:CMF were isolated and cultured from patients undergoing coronary artery bypass grafting.Cultured cells were treated with either TNC(0.1μmol/L,24 h)or a recombinant protein corresponding to different domains of the TNC protein;fibrinogen-like globe(FBG)and fibronectin typeⅢ-like repeats(TNⅢ5-7)(both 1μmol/L,24 h).The expression of the proinflammatory cytokines;interleukin(IL)-6,IL-1β,TNFαand the matrix metalloproteinases;MMPs(MMP1,2,3,9,10,MT1-MMP)was assessed using real time RT-PCR and western blot analysis.RESULTS:TNC increased both IL-6 and MMP3(P<0.01)mR NA levels in cultured human CMF but had no significant effect on the other markers studied.The increase in IL-6 mR NA expression was mirrored by an increase in protein secretion as assessed by enzymelinked immunosorbant assay(P<0.01).Treating CMF with the recombinant protein FBG increased IL-6mR NA and protein(P<0.01)whereas the recombinant protein TNⅢ5-7 had no effect.Neither FBG nor TNⅢ5-7 had any significant effect on MMP3 expression.The expression of toll-like receptor 4(TLR4)in human CMF was confirmed by real time RT-PCR,western blot and immunohistochemistry.Pre-incubation of cells with TLR4neutralising antisera attenuated the effect of both TNC and FBG on IL-6 mR NA and protein expression.CONCLUSION:TNC up-regulates IL-6 expression in human CMF,an effect mediated through the FBG domain of TNC and via the TLR4 receptor. | Azhar Maqbool Emma J Spary Iain W Manfield Michaela Ruhmann Lorena Zuliani-Alvarez Filomena O Gamboa-Esteves Karen E Porter Mark J Drinkhill Kim S Midwood Neil A Turner | 2016 | World Journal of Cardiology2016，8，5： | 4 |
| 2 | Endoscopic findings in patients with Schatzki rings:Evidence for an association with eosinophilic esophagitis显示文摘AIM:To investigate endoscopic findings in patients with Schatzki rings(SRs) with a focus on evidence for eosinophilic esophagitis(EoE).METHODS:We consecutively approached all adult patients scheduled for elective outpatient upper endoscopy for a variety of indications at the German Diagnostic Clinic,Wiesbaden,Germany between July 2007 and July 2010.All patients with endoscopically diagnosed SRs,defined as thin,symmetrical,mucosal structures located at the esophagogastric junction,were prospectively registered.Additional endoscopic findings,clinical information and histopathological findings with a focus on esophageal eosinophilia(≥ 20 eosinophils/high power field) were recorded.The criteria for active EoE were defined as:(1) eosinophilic tissue infiltration ≥ 20 eosinophils/hpf;(2) symptoms of esophageal dysfunction;and(3) exclusion of other causes of esophageal eosinophilia.Gastroesophageal reflux disease was excluded by proton pump inhibitor treatment prior to endoscopy.The presence of ≥ 20 eosinophils/hpf in esophageal biopsies in patients that did not fulfil the criteria of EoE was defined as esophageal hypereosinophilia.RESULTS:A SR was diagnosed in 171(3.3%;128 males,43 females,mean age 66 ± 12.9 years) of the 5163 patients that underwent upper gastrointestinalendoscopy.Twenty of the 116 patients(17%) from whom esophageal biopsies were obtained showed histological hypereosinophilia(≥ 20 eosinophils/hpf).Nine of these patients(8 males,1 female,mean age 49 ± 10 years) did not fulfill all diagnostic criteria of EoE,whereas in 11(9%) patients with ≥ 20 eosinophils/hpf,a definite diagnosis of EoE was made.Three of the 11 patients(27%) with definite EoE had no suspicious endoscopic features of EoE.In contrast,in the 25 patients in whom EoE was suspected by endoscopic features,EoE was only confirmed in 7(28%) patients.Patients with EoE were younger(mean age 41.5 ± 6.5 vs 50.5 ± 11.5 years,P = 0.012),were more likely to have a history of allergies(73% vs 29%,P = 0.007) and complained more often of dysphagia(91% vs 34%,P = 0.004) and food impaction(36% vs 6%,P = 0.007) than patients without EoE.Endoscopically,additional webs were found significantly more often in patients with EoE than in patients without EoE(36% vs 11%,P = 0.04).Furthermore,the SR had a tendency to be narrower in patients with EoE than in those without EoE(36% vs 18%,P = 0.22).The percentage of males(73% vs 72%,P = 1.0) and frequency of heartburn(27% vs 27%,P = 1.0) were not significantly different in both groups.The 9 patients with esophageal hypereosinophilia that did not fulfil the diagnostic criteria of EoE were younger(mean age 49 ± 10 years vs 58 ± 6 years,P = 0.0008) and were more likely to have a history of allergies(78% vs 24%,P = 0.003) than patients with < 20 eosinophils/hpf.Predictors of EoE were younger age,presence of dysphagia or food impaction and a history of allergies.CONCLUSION:A significant proportion of patients with SRs also have EoE,which may not always be suspected according to other endoscopic features. | Michaela Müller Alexander J Eckardt Annette Fisseler-Eckhoff Susanne Haas Ines Gockel Till Wehrmann | 2012 | World Journal of Gastroenterology2012，18，47： | 2 |
| 3 | Is the schatzki ring a unique esophageal entity?显示文摘AIM:To study,whether the association of Schatzki rings with other esophageal disorders support one of the theories about its etiology.METHODS:From 1987 until 2007,all patients with newly diagnosed symptomatic Schatzki rings (SRs) were prospectively registered and followed.All of them underwent structured interviews with regards to clinical symptoms,as well as endoscopic and/or radiographic examinations.Endoscopic and radiographic studies determined the presence of an SR and additional morphological abnormalities.RESULTS:One hundred and sixty-seven patients (125 male,42 female) with a mean age of 57.1±14.6 years were studied.All patients complained of intermittent dysphagia for solid food and 113 (79.6%) patients had a history of food impaction.Patients experienced symptoms for a mean of 4.7±5.2 years before diagnosis.Only in 23.4% of the 64 patients who had endoscopic and/or radiological examinations before their first presentation to our clinic,was the SR previously diagnosed.At presentation,the mean ring diameter was 13.9±4.97 mm.One hundred and sixty-two (97%) patients showed a sliding hiatal hernia.Erosive reflux esophagitis was found in 47 (28.1%) patients.Twenty-six (15.6%) of 167 patients showed single or multiple esophageal webs;five (3.0%) patients exhibited eosinophilic esophagitis;and four (2.4%) had esophageal diverticula.Four (7%) of 57 patients undergoing esophageal manometry had nonspecific esophageal motility disorders.CONCLUSION:Schatzki rings are frequently associated with additional esophageal disorders,which support the assumption of a multifactorial etiology.Despite typical symptoms,SRs might be overlooked. | Michaela Müller Ines Gockel Philip Hedwig Alexander J Eckardt Kathrin Kuhr Jochem Knig Volker F Eckardt | 2011 | World Journal of Gastroenterology2011，17，23： | 2 |
| 4 | Detection of Tumor PIK3 CA Status in Metastatic Breast Cancer Using Peripheral Blood 显示文摘 | Michaela JH Danijela J Evan B | 2012 | Clin Cancer Res2012，18，12： | 1 |
| 5 | Determinants of the capital structures of European SMEs 显示文摘 | HALL G C HUTCHINSON P J MICHAELAS N | 2004 | Journal of Business Finance & Accounting Oxford2004，31，56： | 1 |
| 6 | Longitudinal association be-tween prostatitis and development of benign prostatic hyperplasia显示文摘 | Jennifer L Debra J Michaela E | | 0，，03： | 1 |
| 7 | How can SEMs effectively implement the CSR agenda? a UK case study perspective 显示文摘 | Pavel C Michaela A B Christopher J B | 2004 | Corporate Social Responsibility and Environmental Management2004，11，3： | 1 |
| 8 | Determinants of the Capital Structures of European SMEs显示文摘 | Patrick J Hutchinson Nicos Michaelas | 2004 | Journal of Business Finance & Accounting2004，31，6： | 1 |
| 9 | Leaching of metals from fly ashes in the presence of completing agents显示文摘 | Pavel J Michaela W Tomas L | 2002 | Write Manage2002，22，： | 1 |
| 10 | Development ofICF Core Sets for Multiple Sclerosis显示文摘 | Michaela C Andrea W Jürg K | 2008 | Valens2008，，： | 1 |
| 11 | CD25 - regulatory T cells and tumor immunity 显示文摘 | Emma J Michaela DV Denise G | 2003 | Immunology Letters2003，85，： | 1 |
| 12 | Stressful 'Memories'of plants: Evidence and possible mecha- nisms显示文摘 | BRUCE J A MICHAELA C M JOHNATHAN A N | 2007 | Plant Science2007，173，： | 1 |
| 13 | Leaching of metals from fly ashes in the presence of complexing agents 显示文摘 | PAVEL J MICHAELA W TOMAS L | 2002 | Waste Manage2002，22，： | 1 |
| 14 | Stressful 'memories' of plants: Evidence and possible mechanisms 显示文摘 | Bruce J A Michaela C M Johnathan A N | 2007 | Plant Sci- ence2007，173，： | 1 |
| 15 | CD25-regulatory T cells and tumor immunity显示文摘 | Emma J Michaela D V Denise G | 2003 | Immunol Lett2003，85，2： | 1 |
| 16 | Joint angle and delay estimation Using Shift-Invariance Properties显示文摘 | Alle-Jan V Michaela C V and Arogyaswami J P | 1997 | IEEE Signal Processing Letters1997，4，5： | 1 |
| 17 | CD25-regulatory T cells and tumor immunity 显示文摘 | Emma J Michaela DV Denise G | 2003 | Immunol Lett2003，85，2： | 1 |
| 18 | Deep brain stimulation of the nucleus accumbens for the treatment of addiction显示文摘 | Ulf J. Müller Jürgen Voges Johann Steiner Imke Galazky Hans‐Jochen Heinze Michaela M?ller Jared Pisapia Casey Halpern Arthur Caplan Bernhard Bogerts Jens Kuhn | 2012 | Ann NY Acad Sci2012，，1： | 1 |
| 19 | T Cells Expressing a Chimeric Antigen Receptor That Binds Hepatitis B Virus Envelope Proteins Control Virus Replication in Mice显示文摘 | Karin Krebs Nina B?ttinger Li–Rung Huang Markus Chmielewski Silke Arzberger Georg Gasteiger Clemens J?ger Edgar Schmitt Felix Bohne Michaela Aichler Wolfgang Uckert Hinrich Abken Mathias Heikenwalder Percy Knolle Ulrike Protzer | 2013 | Gastroenterology2013，，2： | 1 |
| 20 | The effects of changing water flow velocity on the formation of biofilms and water quality in pilot distribution system consisting of copper or polyethylene pipes显示文摘 | Markku J L Michaela L Ilkka T | 2006 | Water Research2006，40，11： | 1 |
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