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| 1 | Tenascin C upregulates interleukin-6 expression in human cardiac myofibroblasts via toll-like receptor 4显示文摘AIM:To investigate the effect of Tenascin C(TNC)on the expression of pro-inflammatory cytokines and matrixmetalloproteinases in human cardiac myofibroblasts(CMF).METHODS:CMF were isolated and cultured from patients undergoing coronary artery bypass grafting.Cultured cells were treated with either TNC(0.1μmol/L,24 h)or a recombinant protein corresponding to different domains of the TNC protein;fibrinogen-like globe(FBG)and fibronectin typeⅢ-like repeats(TNⅢ5-7)(both 1μmol/L,24 h).The expression of the proinflammatory cytokines;interleukin(IL)-6,IL-1β,TNFαand the matrix metalloproteinases;MMPs(MMP1,2,3,9,10,MT1-MMP)was assessed using real time RT-PCR and western blot analysis.RESULTS:TNC increased both IL-6 and MMP3(P<0.01)mR NA levels in cultured human CMF but had no significant effect on the other markers studied.The increase in IL-6 mR NA expression was mirrored by an increase in protein secretion as assessed by enzymelinked immunosorbant assay(P<0.01).Treating CMF with the recombinant protein FBG increased IL-6mR NA and protein(P<0.01)whereas the recombinant protein TNⅢ5-7 had no effect.Neither FBG nor TNⅢ5-7 had any significant effect on MMP3 expression.The expression of toll-like receptor 4(TLR4)in human CMF was confirmed by real time RT-PCR,western blot and immunohistochemistry.Pre-incubation of cells with TLR4neutralising antisera attenuated the effect of both TNC and FBG on IL-6 mR NA and protein expression.CONCLUSION:TNC up-regulates IL-6 expression in human CMF,an effect mediated through the FBG domain of TNC and via the TLR4 receptor. | Azhar Maqbool Emma J Spary Iain W Manfield Michaela Ruhmann Lorena Zuliani-Alvarez Filomena O Gamboa-Esteves Karen E Porter Mark J Drinkhill Kim S Midwood Neil A Turner | 2016 | World Journal of Cardiology2016，8，5： | 4 |
| 2 | Nitrososphaera viennen- sis, an ammonia oxidizing archaeon from soil显示文摘 | Fourna M Michaela S Anja S etal | 2011 | Proceedings of the National Academy of Sciences2011，108，20： | 1 |
| 3 | 1,4-Substituted 4-(1H)-pyridylene-hydrazone-type inhibitors of ACh BuChE, and amyloid-β aggregation crossing the blood-brain barrier显示文摘 | Michaela P Sülünay P Gülsah B | 2013 | Eur J Pharmaceut Sci2013，49，12： | 1 |
| 4 | Specific inhibition of bcr-abl gene expression by small interfering RNA显示文摘 | Michaela S Battmer K Winkler T | 2003 | Blood2003，101，4： | 1 |
| 5 | High-Performance Liquid-Chromatographic determination of free nicotinic acid and its metabolite,nicotinuric acid, in plasma and urine显示文摘 | Nikolaus H Volker S Michaela H | 1978 | Clinical Chemistry1978，24，： | 1 |
| 6 | Roughley,MMPs are less efficient than ADAMTS5 in cleaving aggrecan core protein显示文摘 | Michaela D Hideaki N John S | 2011 | Matrix Biology2011，30，3： | 1 |
| 7 | Relation between the molecular structure and the efficiency of superabsorbent poly- mer (SAP) as concrete admixture to mitigate autogenousshrinkage显示文摘 | Christof S Viktor M Michaela G | 2012 | Cement and Concrete Research2012，，42： | 1 |
| 8 | Differencein risk factors for postoperative nausea and vomiting显示文摘 | Michaela S | 2003 | Anesthesiology2003，98，1： | 1 |
| 9 | Circular dichroism and guanine quadmplexes显示文摘 | Michaela V Iva K Janos S | 2012 | Methods2012，57，1： | 1 |
| 10 | Nitrososphaera viennensis, an ammonia oxidizing archaeon from soil 显示文摘 | MARIA T MICHAELA S ANJA S | 2011 | Proceedings of the National Academy of Sciences of the United States of America2011，108，20： | 1 |
| 11 | Knowledge sharing and collabora-tion in translation research and the DC-THERA directory 显示文摘 | ANDREA S MICHAELA G | 2011 | Briefings in Bioinformatics2011，12，6： | 1 |
| 12 | Degradation offumonisin B1 by the consecutive action of two bacterial enzymes显示文摘 | Heinl S Doris H Michaela T | | 0，，02： | 1 |
| 13 | Determination of sorbic acid in urine by gas chromatography-mass spectrometry显示文摘 | Thomas R Michaela Baer-Koetzle Gerhard S | 1999 | J Chromatogr A1999，847，12： | 1 |
| 14 | Specific inhibition of bcr/abl gene expression by small interfering RNA显示文摘 | Battmer K Winkler T | 2003 | Blood2003，101，4： | 1 |
| 15 | Work group diversity显示文摘 | Daan V K Michaela S | 2007 | Annual Review of Psychology2007，58，1： | 1 |
| 16 | Quantitative determination of carotene stereoisomers in carrotjuices and vita2min supplemented (ATBC) drinks 显示文摘 | Michaela M Andreas S Reinhold C | 2000 | Food Chem2000，70，： | 1 |
| 17 | Impact of additional chromosomal aberrations and BCR-ABL kinase domain mutations on the response to nilotinib in Philadelphia chromosome-positive chronic myeloid leukemia显示文摘 | Theo D K Seval T Michaela S | 2010 | haematologica2010，95，4： | 1 |
| 18 | Phosphoryla- tion-mediated negative regulation of RIG-1 antiviral activity 显示文摘 | Michaela U G estanislao N V Inn K S | 2010 | J Virol2010，84，7： | 1 |
| 19 | Quantitative determination of carotene stereoisomers in carrot juices and vitamin supplemented(ATBC)drinks显示文摘 | MICHAELA M ANDREAS S REINHOLD C | 2000 | Food Chem2000，70，： | 1 |
| 20 | Concentrations of gatifloxacin in plasma and urine and penetration into prostatic and seminal fluid, ejaculate and sperm cells after single oral administration of 400mg to volunteers显示文摘 | Christoph KN Michaela S Martina KS | | Antimicrob Agents chemot0，，： | 1 |
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