| 2 | Deficiency of Adenomatous Polyposis Coli protein in sporadic colorectal adenomas and its associations with clinical phenotype and histology显示文摘瞄准:评估腺瘤结肠息肉病(APC ) 蛋白质的损失的频率并且把 APC 地位与颜色的特征作比较表面的腺瘤。方法:APC 蛋白质的 Immunohistochemical 分析在 118 个腺瘤上被执行,结果与病人的恶意的潜力,腺瘤的地点,宏观的外观和年龄的参数相比。结果:APC 蛋白质的完全的损失在 28 被发现的 A (24%) 腺瘤,当(76%) 90 是 APC 时积极。腺瘤的吝啬的尺寸是 13.5 +/- 14.2 公里(95% CI 10.5-16.5 ) 在 APC 积极,并且 13.8 +/- 在 APC 否定的腺瘤的 15.5 公里(95% CI 7.8-19.8 )(P = 0.364 ) 。统计分析没至于组织学的类型揭示 APC 积极、否定的腺瘤之间的差别(P = 0.327 ) 并且发育异常的等级(P = 0.494 ) 。我们发现甚至先进的腺瘤没与非先进的肿瘤在他们的 APC 地位不同(P = 0.414 ) 。最后,当时,没有差别被发现地点(P = 0.157 ) ,宏观的外观(P = 0.571 ) 并且病人的年龄(P = 0.438 ) 在两个 APC 积极、否定的腺瘤之间被分析并且比较。结论:大多数腺瘤表示了全身的 APC 蛋白质,建议那蛋白质表情不是为对 APC 基因变化的评价的一个可靠标记。APC 蛋白质的完全的损失没影响腺瘤的形态学,地点,或外观,它也不受病人的年龄影响。 | Martin Bortlík Ivana Vítková Martina Pape(z|ˇ)ová Milada Kohoutová Ale(s|ˇ) Novotn(y|') Stanislav Adamec Petra Chalupná Milan Luká(s|ˇ) | 2006 | World Journal of Gastroenterology2006，12，24： | 1 |
| 4 | Application of comprehensive two-dimensional gas chromatography with masss pectrometric detection for the analysis of selected drug residues in wastewater and surface water显示文摘Pharmaceutical residues have become tightly controlled environmental contaminants in recent years, due to their increasing concentration in environmental components. This is mainly caused by their high level of production and everyday consumption. Therefore there is a need to apply new and sufficiently sensitive analytical methods, which can detect the presence of these contaminants even in very low concentrations. This study is focused on the application of a reliable analytical method for the analysis of 10 selected drug residues, mainly from the group of non-steroidal anti-inflammatory drugs (salicylic acid, acetylsalicylic acid, clofibric acid, ibuprofen, acetaminophen, caffeine, naproxen, mefenamic acid, ketoprofen, and dicofenac), in wastewaters and surface waters. This analytical method is based on solid phase extraction, derivatization by N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA) and finally analysis by comprehensive two-dimensional gas chromatography with Time-of-Flight mass spectrometric detection (GC×GC- TOF MS). Detection limits ranged from 0.18 to 5 ng/L depending on the compound and selected matrix. The method was successfully applied for detection of the presence of selected pharmaceuticals in the Svratka River and in wastewater from the wastewater treatment plant in Brno-Modrice, Czech Republic. The concentration of pharmaceuticals varied from one to several hundreds of ng/L in surface water and from one to several tens of μg/L in wastewater. | Petr Lacina Ludmila Mravcov Milada Vvrov | 2013 | Journal of Environmental Sciences2013，25，1： | 1 |
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