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| 1 | Proteomics-based Identification of Proteins with Altered Expression Induced by 12-O-Tetradecanoylphorbol 13-acetate In Nasopharyngeal Carcinoma CNE2 Cells显示文摘Nasopharyngeal carcinoma (NPC) is a malignancy with high incidence in Southern China and South-East Asia. Etiology studies indicate that chemical carcinogen promoters, such as 12-O-tetradecanoylphorbol-13-acetate (TPA), are important factors causing NPC development. However, the mechanism of the TPA effect on NPC remains unclear. In the present study, cells from a poorly differentiated squamous cell carcinoma NPC cell line, CNE2, were stimulated by TPA and proteomics technology was carried out to find protein discrepancies between control and TPA-treated cells. Results revealed that TPA treatment in CNE2 cells could upregulate the expression of ''''triosephosphate isomerase'''' and ''''14-3-3 protein sigma'''' and downregulate the expression of ''''reticulocalbin 1 precursor'''', ''''nucleophosmin'''', ''''mitochondrial matrix protein pl precursor'''', and ''''stathmin''''. The changes in the expression of these genes suggested that TPA induced CNE2 cells to antiproliferation and to apoptosis, which was confirmed by subsequent apoptosis detection. Therefore, the effects of TPA on nasopharyngeal carcinoma cells were distinct from the effects on primary epithelial cells and we suggest reasons for these differences. | Pei-ZhouJIANG MingGAN HuaHUANG Xin-MingSHEN ShuangWANG Kai-TaiYAO | 2005 | Acta Biochimica et Biophysica Sinica2005，37，2： | 5 |
| 2 | THE ROLE OF BACTERIAL SYMBIONTS IN AMINO ACID COMPOSITION OF BLACK BEAN APHIDS显示文摘To evaluate the role of bacterial symbionts ( Buchnera spp. ) in the black bean aphids ( Aphis craccivora Koch), the aphids were treated with the antibiotic, rifampicin, to eliminate their intracellular symbiotic bacteria. Analysis of protein and amino acid concentration in 7-day-old of aposymbiotic aphids showed that the total protein content per mg fresh weight was significantly reduced by 29 %, but free amino acid titers were increased by 17% . The ratio of the essential amino acids was in general only around 20% essential amino acids in phloem sap of broad bean, whereas it was 44% and 37% in symbiotic and aposymbiotic aphids, respectively,suggesting that the composition of the free amino acids was unbalanced. For example, the essential amino acid,threonine represented 21. 6% of essential amino acids in symbiotic aphids, but it was only 16.7% in aposymbiotic aphids. Likewise, two nonessential amino acids, tyrosine and serine, represented 8.9% and 5.6% of total amino acids in symbiontic aphids, respectively, but they enhanced to 21.1% and 13.6% in aposymbiotic aphids. It seems likely that the elevated free amino acid concentration in aposymbiotic aphids was caused by the limited protein anabolism as the result of the unbalanced amino acid composition. | MingGan De-ChengDing Xue-xiaMiao | 2003 | Entomologia Sinica2003，10，3： | 1 |
| 3 | Adaptive robust control for DC motors with input saturation 显示文摘 | LI Zhiping CHEN Jie ZHANG Guozhu GAN Minggan | 2011 | IET Control Theory and Applications2011，5，16： | 1 |
| 4 | Recent patents in fluid dispensing processes for electronics packaging显示文摘 | MINGGAN L CHEN X B | 2009 | Recent Patents on Mechanical Engineering2009，2，： | 1 |
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