|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Modification in Media Composition to Obtain Secretory Production of STxB-based Vaccines using Escherichia coli显示文摘Shiga toxin B-subunit (STxB) from Shigella dysenteriae targets in vivo antigen to cancer cells, dendritic cells (DC) and B cells, which preferentially express the globotriaosylceramide (Gb3) receptor. This pivotal role has encouraged scientists to investigate fusing STxB with other clinical antigens. Due to the challenges of obtaining a functional soluble form of the recombinant STxB, such as formation of inclusion bodies during protein expression, scientists tend to combine STxB with vaccine candidates rather than using their genetically fused forms. In this work, we fused HPV16 E7 as a vaccine candidate to the recombinantly-produced STxB. To minimize the formation of inclusion bodies, we investigated a number of conditions during the expression procedure. Then various strategies were used in order to obtain high yield of soluble recombinant protein from E. coli which included the use of different host strains, reduction of cultivation temperature, as well as using different concentrations of IPTG and different additives (Glycin, Triton X-100, ZnCl2). Our study demonstrated the importance of optimizing incubation parameters for recombinant protein expression in E. coli; also showed that the secretion production can be achieved over the course of a few hours when using additives such as glycine and Triton X-100. Interestingly, it was shown that when the culture mediums were supplemented by additives, there was an inverse ratio between time of induction (TOI) and the level of secreted protein at lower temperatures. This study determines the optimal conditions for high yield soluble E7-STxB expression and subsequently facilitates reaching a functionally soluble form of STxB-based vaccines, which can be considered as a potent vaccine candidate for cervical cancer. | Mohammad Sadraeian Mohammad Bagher Ghoshoon Milad Mohkam Zeinab Karimi SaraRasoul-Amini Younes Ghasemi | 2013 | Virologica Sinica2013，28，1： | 7 |
| 2 | Intra-operative peritoneal lavage for colorectal cancer显示文摘Free cancer cells can be detected in peritoneal fluid at the time of colorectal surgery.Peritoneal lavage in colorectal surgery for cancer is not used in routine,and the prognostic significance of intraperitoneal free cancer cells(IPCC)remains unclear.Data concerning the technique of peritoneal lavage to detect IPCC and its timing regarding colorectal resection are scarce.However,positive IPCC might be the first step of peritoneal spread in colorectal cancers,which could lead to early specific treatments.Because of the important heterogeneity of IPCC determination in reported studies,no treatment have been proposed to patients why positive IPCC.Herein,we provide an overview of IPCC detection and its impact on recurrence and survival,and we suggest further multi-institutional studies to evaluate new treatment strategies. | Guillaume Passot Kayvan Mohkam Eddy Cotte Olivier Glehen | 2014 | World Journal of Gastroenterology2014，20，8： | 5 |
| 3 | Technetium Te99m dimercaptosaceinic acid renal scintigraphy in children with acute pyelonephritis: Correlation with other imaging tests 显示文摘 | Mohkam M Maham S Rahmani A | 2010 | Iran J Kidney Dis2010，4，4： | 1 |
| 4 | Ceftriaxone associated nephrolithiasis:a prospective study in 284 children显示文摘 | Mohkam M Karimi A Gharib A | 2007 | Pediatr Nephrol2007，22，5： | 1 |
| 5 | Ceftriaxone associated nephrolithiasis:a prospective study in 284 children显示文摘 | Mohkam M Karimi A Gharib A | 2007 | Pediatr Nephrol2007，22，： | 1 |
| 6 | Ceftriaxone associated nephroli- thiasis: a prospective study in 284 children 显示文摘 | Mohkam M Karimi A Gharib A | 2007 | Pediatric nephrology (Berlin Germany)2007，22，5： | 1 |
| 7 | Ceftriaxone associated nephrolithiasis:a prospective study in 284 children显示文摘 | Mohkam M Karimi A Gharib A | 2007 | Pediatr Nephrol2007，22，5： | 1 |
| 8 | Rapid antibody test for fragile X syndrome显示文摘 | Willemsen R Mohkaming S de Vries B | 1995 | Lancet1995，345，8958： | 1 |
| 9 | Optimization of Three Dimensional Culturing of the HepG2 Cell Line in Fibrin Scaffold 显示文摘 | Banihashemi M Mohkam M Safari A | 2015 | Hepat Mon2015，15，22： | 1 |
| 10 | Ceftriaxone associated nephrolithiasis:a prospective study in 284 children显示文摘 | Mohkam M Karimi A Gharib A | 2007 | Pediatr Nephrol2007，22，5： | 1 |
| 11 | Ceftriaxone associated nephrolithiasis:a prospective study in 284 children显示文摘 | Mohkam M Karimi A Gharib A | 2007 | Pediatr Nephrol2007，22，5： | 1 |
| 12 | Ceftriaxone associated nephrolithiasis: a prospective study in 284 children显示文摘 | Masoumeh Mohkam Abdollah Karimi Atoosa Gharib Hamid Daneshmand Alireza Khatami Nozar Ghojevand Mostafa Sharifian | 2007 | Pediatric Nephrology2007，，5： | 1 |
| 13 | Surgical Management of Liver Hydatid Disease: Subadventitial Cystectomy versus Resection of the Protruding Dome 显示文摘 | Mohkam K Belkhir L Wallon M | 2014 | World J Surg2014，38，8： | 1 |
| 14 | Ceftriaxone associated nephrolithiasis: a prospective study in 284 ehildren显示文摘 | Mohkam M Karimi A Gharib A | 2007 | Pediatr Neprol2007，22，5： | 1 |
| 15 | Prevention and inhibition of TC-1 cell growth in tumor beating mice by HPV16 E7 protein in fusion with Shiga toxin B-subunit from shigella dysenteriae显示文摘 | Sadraeian M Khoshnood Mansoorkhani MJ Mohkam M | | 0，，2： | 1 |
| 16 | Ceftriaxone associated nephrolithiasis:a prospective study in 284 children显示文摘 | Mohkam M Karimi A Gharib A | 2007 | Pediatr Nephrol2007，22，5： | 1 |
| 17 | Ceftriaxone associ- ated nephrolithiasis: a prospective study in 284 children 显示文摘 | Mohkam M Karimi A Gharib A | 2007 | Pediatr Nephrol2007，22，： | 1 |
| 18 | Cettriaxone associated nephrolithiasis: a prospective study in 284 children显示文摘 | Mohkam M Karimi A Gharib A | 2007 | Pediatr Nephrol2007，22，5： | 1 |
| 19 | Double cholecystectomy in case of accessory gallbladder:Not as easy as two cholecystectomies显示文摘To the Editor:Accessory gallbladders(AG)are rare and their management are usually challenging.These variations are related with an abnormal division of the bile ducts precursors between the 5th and the 12th week of pregnancy.Harlaftis et al.[1]proposed an anatomical classification of these variations based on the embryologic abnormalities described.In type 1 both gallbladders are connected to the common bile duct at the same location.In type 2 the AG can reach either the common bile duct(ductular type)or an intrahepatic biliary duct(trabecular type).In type 3,three gallbladders are present. | Benjamin Darnis Kayvan Mohkam Fran?ois Cauchy Jean-Yves Mabrut | 2019 | Hepatobiliary & Pancreatic Diseases International2019，18，1： | 1 |
| 20 | Ceftriaxone associated nephrolithiasis:a prospective study in284children显示文摘 | Mohkam M Karimi A Gharib A Daneshmand H Khatami A Ghojevand N | 2007 | Pediatr Nephrol2007，22，5： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员