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| 1 | Effect of Helicobacter pylori cdrA on interleukin-8 secretions and nuclear factor kappa B activation显示文摘AIM:To investigate genetic diversity of Helicobacter pylori (H.pylori) cell division-related gene A (cdrA) and its effect on the host response.METHODS:Inactivation of H.pylori cdrA,which is involved in cell division and morphological elongation,has a role in chronic persistent infections.Genetic property of H.pylori cdrA was evaluated using polymerase chain reaction and sequencing in 128 (77 American and 51 Japanese) clinical isolates obtained from 48 and 51 patients,respectively.Enzyme-linked immunosorbent assay was performed to measure interleukin-8 (IL-8) secretion with gastric biopsy specimens obtained from American patients colonized with cdrA-positive or-negative strains and AGS cells cocultured with wild-type HPK5 (cdrA-positive) or its derivative HPKT510 (cdrA-disruptant).Furthermore,the cytotoxin-associated gene A (cagA) status (translocation and phosphorylation) and kinetics of transcription factors [nuclear factor-kappa B (NF-κB) and inhibition kappa B] were investigated in AGS cells co-cultured with HPK5,HPKT510 and its derivative HPK5CA (cagA disruptant) by western blotting analysis with immunoprecipitation.RESULTS:Genetic diversity of the H.pylori cdrA gene demonstrated that the cdrA status segregated into two categories including four allele types,cdrA-positive (allele types;Ⅰand Ⅱ) and cdrA-negative (allele types;Ⅲ and Ⅳ) categories,respectively.Almost all Japanese isolates were cdrA-positive (Ⅰ:7.8% and Ⅱ:90.2%),whereas 16.9% of American isolates were cdrA-positive (Ⅱ) and 83.1% were cdrA-negative (Ⅲ:37.7% and Ⅳ:45.5%),indicating extended diversity of cdrA in individual American isolates.Comparison of each isolate from different regions (antrum and corpus) in the stomach of 29 Americans revealed that cdrA status was identical in both isolates from different regions in 17 cases.However,12 cases had a different cdrA allele and 6 of them exhibited a different cdrA category between two regions in the stomach.Furthermore,in 5 of the 6 cases possessing a different cdrA category,cdrA-negative isolate existed in the corpus,suggesting that cdrA-negative strain is more adaptable to colonization in the corpus.IL-8 secretions from AGS revealed that IL-8 levels induced by a cdrA-disrupted HPKT510 was significantly lower (P < 0.01) compared to wildtype HPK5:corresponding to 50%-60% of those of wild-type HPK5.These data coincided with in vivo data that an average value of IL-8 in biopsy specimens from cdrA-positive and cdrA-negative groups was 215.6 and 135.9 pg/mL,respectively.Western blotting analysis documented that HPKT510 had no effect on CagA translocation and phosphorylation,however,nuclear accumulation of NF-κB was lower by HPKT510 compared to HPK5.CONCLUSION:Colonization by a cdrA-negative or cdrA-dysfunctional strain resulted in decreased IL-8 production and repression of NF-κB,and hence,attenuate the host immunity leading to persistent infection. | Hiroaki Takeuchi Ya-Nan Zhang Dawn A Israel Richard M Peek Jr Mikio Kamioka Hideo Yanai Norihito Morimoto Tetsuro Sugiura | 2012 | World Journal of Gastroenterology2012，18，5： | 3 |
| 2 | Cyclosporine induces cancer progression by a cell-autonomous mechanism (see comments)显示文摘 | Hojo M Morimoto T Maluccio M | | 0，，： | 2 |
| 3 | Kindling and status epilepticus models of epilepsy: rewiring the brain 显示文摘 | Morimoto K Fahnestock M Racine RJ | 2004 | Prog Neurobiol2004，73，1： | 1 |
| 4 | Obstructive jaundice increases sensitivity to lipopolysaccharide via TLR4 up regulation: possible involvement in gut-derived hepatocyte growth factorpretection of hepatocytes 显示文摘 | Miyaso H Morimoto Y Ozaki M | 2005 | J Gastroentere 1 Hepatol2005，20，12： | 1 |
| 5 | Experimental antitumor activity of Navelbine显示文摘 | Cros S Wright M Morimoto M | 1989 | Semin Oncol1989，16，24： | 1 |
| 6 | Hepatocyte-targeted in vivo gene expression by intra venous injection of plasmid DNA complexed with synthetic multi-functional gene delivery system 显示文摘 | Nishikawa M Yamauchi M Morimoto K | 2000 | Gene Ther2000，7，7： | 1 |
| 7 | A new rabbit model of myocardial infarction without endotracheal intubation 显示文摘 | Fujita M Morimoto Y Ishihara M | 2004 | J Surg Res2004，116，1： | 1 |
| 8 | Parental rearing attitudes and health-relat- ed lifestyle of university studenta 显示文摘 | TODA M MORIMOTO K | 2008 | Soc Behav Personal2008，36，4： | 1 |
| 9 | Structural requirements for the flavonoid fisetin in inhibiting fibril forma-tion of amyloid p protein显示文摘 | Akaishi T Morimoto T Shibao M | 2008 | Neurosci Lett2008，444，3： | 1 |
| 10 | Efficacy of fusion imaging combining sonography and hepatobiliary phase MRI with Gd- EOB-DTPA to detect small hepatocellular carcinoma显示文摘 | Kunishi Y Numata K Morimoto M | 2012 | AJR Am J Roentgenol2012，198，1： | 1 |
| 11 | Rabbit model of airway stenosis induced by scraping of the tracheal mucosa显示文摘 | NAKAGISHI Y MORIMOTO Y FUJITA M | 2005 | Laryngoscope2005，115，6： | 1 |
| 12 | Hypertension is associated with serologically active disease in patients with systemic lupus erythematosus:role of increased Th1/Th2ratio and oxidative stress显示文摘 | Lozovoy M A Simo A N Morimoto H K | 2014 | Scand J Rheumatol2014，43，1： | 1 |
| 13 | Eye Gaze Tracking Techniques for Interactive Applications显示文摘 | MORIMOTO C H MIMICA M R M | 2005 | Journal of Computer Vision and Image Understanding Special Issue on Eye Detection and Tracking2005，98，1： | 1 |
| 14 | Inhibitory effect of tachyplesin I on the proliferation of human immunodeficiency virus in vitro显示文摘 | Morimoto M Mori H Otak T | 1991 | Chemotherapy1991，37，3： | 1 |
| 15 | Structure of FR-900359 a cyclic depsipptide from Ardisia crenata Sims显示文摘 | FUJIOKA M KODA S MORIMOTO Y | 1988 | J Org Chem1988，53，12： | 1 |
| 16 | Continuous ATP regeneration using immobilized acetone-dried yeast cells显示文摘 | Asada M Morimoto K | 1979 | Agric Biol Chem1979，43，8： | 1 |
| 17 | Splicing isoformof SYT-SSX fusion protein accelerates transcriptional activity andcell proliferation显示文摘 | Morimoto Y Ouchida M Ozaki T | 2003 | Cancer Lett2003，199，1： | 1 |
| 18 | Combination therapy with short interfering RNA vectors against VEGF-C and VEGF-A suppresses lymph node and lung metastasis in a mouse immunocompetent mammary cancer model显示文摘 | Shibata M Morimoto J Shibata E | | 0，，： | 1 |
| 19 | Fucosylated Fraction of Alpha- fetoprotein as a Serological Marker of Early Hepatocellular Carcinoma 显示文摘 | Moriya S Morimoto M Numata K | 2013 | Antieancer Res2013，33，3： | 1 |
| 20 | KB-R7785, a novel matrix metalloproteinase inhibitor, exerts its antidiabetic effect by inhibiting tumor necrosis factor-alpha production显示文摘 | Nishikawa K Ohashi M | 1997 | Life Sci1997，61，8： | 1 |
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