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| 1 | Effect of adipose-derived mesenchymal stem cells on hepatocellular carcinoma: In vitro inhibition of carcinogenesis显示文摘AIM To investigate the effect of adipose-derived mesenchymal stem cells(ADMSCs)and their conditioned media(CM) on hepatocellular carcinoma(HCC) cell tumorigenesis.METHODS The proliferation rate of HepG2 and PLC-PRF-5 HCC cancer cells was measured using the trypan blue exclusion method and confirmed using the cell-counting kit8(commonly known as CCK-8) assay. Apoptosis was detected by flow cytometry using annexin V-FITC. Protein and mRNA expression was quantified by ELISA and real time PCR, respectively. Migration and invasion rates were performed by Transwell migration and invasion assays. Wound healing was examined to confirm the data obtained from the migration assays.RESULTS Our data demonstrated that when co-culturing HCC cell lines with ADMSCs or treating them with ADMSC CM, the HCC cell proliferation rate was significantly inhibited and the apoptosis rate increased. The decreased proliferation rate was accompanied by an upregulation of P53 and Retinoblastoma mRNA and a downregulation of c-Myc and hTERT mRNA levels. More notably, ADMSCs and their CM suppressed the expression of the two important markers of HCC carcinogenicity, alpha-fetoprotein and Des-gamma-carboxyprothrombin. In addition, the migration and invasion levels of HepG2 and PLC-PRF-5 cells significantly decreased, potentially through increased expression of the tissue inhibitor metalloproteinases TIMP-1, TIMP-2 and TIMP-3.CONCLUSION These findings shed new light on a protective and therapeutic role for ADMSCs and their CM in controlling HCC invasiveness and carcinogenesis. | Rim Serhal Nagib Saliba George Hilal Mayssam Moussa Ghada S Hassan Oula El Atat Nada Alaaeddine | 2019 | World Journal of Gastroenterology2019，25，5： | 6 |
| 2 | Involvement of polyamines in the chilling tolerance of cucumber cultivars显示文摘 | Shen W Nada K Tachibana S | 2000 | Plant Physiology2000，124，1： | 1 |
| 3 | Ulcerative colitis after renal transplantation: a case report and reviewof literature 显示文摘 | Parameswaran S Singh K Nada R | 2011 | Indian J Nephrol2011，21，2： | 1 |
| 4 | Differences between tomato and cucumber in ethanol,lactate and malate metabolisms and cell sap pH of roots under hypoxia显示文摘 | GUO S R NADA K KATOH H TACHIBANA S | 1999 | Journal of Japan Society Horticulture Science1999，68，1： | 1 |
| 5 | Involvement of polyamines in the chilling tolerance of cucumber cultivars显示文摘 | SHEN W NADA K TACHIBANA S | 2000 | Plant Physiol2000，124，： | 1 |
| 6 | The mTOR pathway controls cell proliferation by regulating the Fox03a transcription factor via SGKI kinase显示文摘 | Mori S Nada S Kimura H | 2014 | PLoS One2014，9，88： | 1 |
| 7 | Involvement of polyamines in the chilling tolerance of cucumber cultivars显示文摘 | Shen W Y Nada K Tachibana S | 2000 | Plant Physiology2000，124，： | 1 |
| 8 | Peroxyformic acid pulping of palm leaves显示文摘 | A M A Nada H Abou-Yousef S Kamel | 2004 | Journal of Scientific &Industrial Research2004，，63： | 1 |
| 9 | Abnormalities of enteric neurons,intestinal pacemaker cells,and smooth muscle in human intestinal atresia显示文摘 | Suita S Nada O | 1999 | J Pediatr Surg1999，34，10： | 1 |
| 10 | Infrared spectroscopic study of lignins 显示文摘 | NADA A M A EI-SAKHAWY M KAMEL S M | 1998 | Polymer Degradation and Stability1998，60，： | 1 |
| 11 | Characterization of adeno-associated virus rep protein inhibition of adenovirus E2a gene expression显示文摘 | Nada S Trempe J P | 2002 | Virology2002，293，： | 1 |
| 12 | Double common bile duct: A case report显示文摘Double common bile duct (DCBD) is a rare congenital anomaly in which two common bile ducts exist. One usually has normal drainage into the papilla duodeni major and the other usually named accessory common bile duct (ACBD) opens in different parts of upper gastrointestinal tract (stomach, duodenum, ductus pancreaticus or septum). This anomaly is of great importance since it is often associated with biliary lithiasis, choledochal cyst, anomalous pancreaticobiliary junction (APBJ) and upper gastrointestinal tract malignancies. We recently recognized a rare case of DCBD associated with APBJ with lithiasis in better developed common bile duct. The opening site of ACBD was in the pancreatic duct. The anomaly was suspected by transabdominal ultrasonography and finally confirmed by endoscopic retrograde cholangiopancreatography (ERCP) followed by endoscopic sphincterotomy and stone extraction. According to the literature, the existence of DCBD with the opening of ACBD in the pancreatic duct is most frequently associated with APBJ and gallbladder carcinoma. In case of DCBD, the opening site of ACBD is of greatest clinical importance because of its close implications with concomitant pathology. The adequate diagnosis of this rare anomaly is significant since the operative complications may occur in cases with DCBD which is not recognized prior to surgical treatment. | Srdjan P Djuranovic Milenko B Ugljesic Nenad S Mijalkovic Viktorija A Korneti Nada V Kovacevic Tamara M Alempijevic Slaven V Radulovic Dragan V Tomic Milan M Spuran | 2007 | World Journal of Gastroenterology2007，13，27： | 1 |
| 13 | Rat pulmonary circulation after chronic hypoxia:hemodynamic and structural features显示文摘 | RABINOVITCH M GAMBLE W NADAS A S | 1979 | Am J Physiol(Heart Circ Physiol)1979，236，： | 1 |
| 14 | Moderate Treadmill Exercise Prevents Oxidative Stress-Induced Anxiety-Like Behavior In Rats 显示文摘 | SAMINA S NADA S MANISH T | 2010 | Behavioural Brain Research2010，208，： | 1 |
| 15 | Long-term results of subtotal vs total parathyroidectomy without autotransplantation in kidney transplant recipients显示文摘 | Nada R Daniel S Ralf S | 2008 | Arch Surg2008，143，8： | 1 |
| 16 | Formation and function of secondary aerechyma in hypocotyl, roots and nodules of soybean ( Glycine max) under flooded conditions 显示文摘 | Shimamura S Mochizuki T Nada Y | 2003 | Plant and Soil2003，251，： | 1 |
| 17 | Early enteral supply of fiber and lactobatilli versus conventional nutrition:A controlled trial in patients with major abdominal surgery显示文摘 | Nada R Sonja H Daniel S | 2002 | Nutrition2002，18，： | 1 |
| 18 | Slot/slots air jet impinging cooling of a cylinder for different jets-cylinder configurations显示文摘 | Nada S A | 2006 | Heat and Mass Transfer2006，43，2： | 1 |
| 19 | Thermal behaviour and infrared spectroscopy of cellulose carbamates显示文摘 | Nada A M A Kamel S Mohamed El-Sakhawy | 2000 | Polymer Degradation and Stability2000，70，： | 1 |
| 20 | Evaluation of candidate reference genes for expression studies in Pisum sativum under different experimental conditions显示文摘 | Die J V Roman B Nada S | | 0，，01： | 1 |
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