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| 1 | Drug sensitivity and drug resistance profiles of human intrahepatic cholangiocarcinoma cell lines显示文摘AIM: To study the effect of a number of chemotherapeutic drugs on five human intrahepatic cholangiocarcinoma (CCA) cell lines. The expressions of genes that have been proposed to influence the resistance of chemotherapeutic drugs including thymidylate synthase (TS), dihydropyrimidine dehydrogenase (DPD), glutathione-S-transferase P1 (GSTP1), multidrug resistance protein (MDR1) and multidrug resistance-associated proteins (MRPs) were also determined.METHODS: Five human CCA cell lines (KKU-100, KKU M055, KKU-M156, KKU-M214 and KKU-OCA17) weretreated with various chemotherapeutic drugs and growth inhibition was determined by 3-(4,5-dimethylthiazol-2-yl)5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. Semi-quantitative levels of gene expression were determined by a reverse transcriptase polymerase chain reaction (RT-PCR). Results of IC50 values and the ratios of gene expression were analyzed by linear regression to predict their relationship. RESULTS: Among five CCA cell lines, KKU-M055 was the most sensitive cell line towards all chemotherapeutic drugs investigated, particularly taxane derivatives with IC50 values of 0.02-3 nmol/L, whereas KKU-100 was apparently the least sensitive cell line. When compared to other chemotherapeutic agents, doxorubicin and pirarubicin showed the lowest IC50 values (<5 μmol/L) in all five CCA cell lines. Results from RT-PCR showed that TS, MRP1, MRP3 and GSTP1 were highly expressed in these five CCA cell lines while DPD and MRP2 were only moderately expressed. It should be noted that MDR1 expression was detected only in KKU-OCA17 cell lines. A strong correlation was only found between the level of MRP3 expression and the IC50 values of etoposide, doxorubicin and pirarubicin (r = 0.86-0.98, ,P<0.05). CONCLUSION: Sensitivity to chemotherapeutic agents is not associated with the histological type of CCA. Choosing of the appropriate chemotherapeutic regimen for the treatment of CCA requires knowledge of drug sensitivity. MRP3 was correlated with resistance of CCA cell lines to etoposide, doxorubicin and pirarubicin, whereas other chemotherapeutic drugs showed no association. The roleof this multidrug resistance-associated protein, MRP3, in chemotherapeutic resistance in CCA patients needs to be further investigated. | Nisana Tepsiri Liengchai Chaturat Banchob Sripa Wises Namwat Sopit Wongkham Vajarabhongsa Bhudhisawasdi Wichittra Tassaneeyakul | 2005 | World Journal of Gastroenterology2005，11，18： | 10 |
| 2 | Apoptotic activity of caged xanthones from Garcinia hanburyi in cholangiocarcinoma cell lines显示文摘AIM:To investigate the growth inhibitory mechanism of four caged xanthones from Garcinia hanburyi in cholangiocarcinoma(CCA) KKU-100 and KKU-M156 cells.METHODS:Four caged xanthones,selected on the basis of their anticancer potency and chemical structure diversities(i.e.isomorellin,isomorellinol,forbesione and gambogic acid) were used in this study.Growth inhibition of these caged xanthones was determined using the sulforhodamine B assay.Induction of apoptosis was assessed by observing cell morphology,ethidium bromide and acridine orange staining and DNA fragmentation assay.Levels of apoptotic-related gene and protein expressions were determined by a real-time reverse transcriptase polymerase chain reaction and Western blotting analysis,respectively.RESULTS:The compounds were found to inhibit growth of both cell lines in a dose-dependent manner and also showed selective cytotoxicity against the cancer cells when compared with normal peripheral blood mononuclear cells.Growth suppression by these compounds was due to apoptosis,as evidenced by the cell morphological changes,chromatin condensation,nuclear fragmentation,and DNA ladder formation.At the molecular level,these compounds induced down-regulation of Bcl-2 and survivin proteins with up-regulation of Bax and apoptosisinducing factor proteins,leading to the activation of caspase-9 and-3 and DNA fragmentation.The functional group variations did not appear to affect the anticancer activity with regard to the two CCA cell lines;however,at a mechanistic level,isomorellinol exhibited the highest potency in increasing the Bax/Bcl-2 protein expression ratio(120 and 41.4 for KKU-100 and KKU-M156,respectively) and in decreasing survivin protein expression(0.01 fold as compared to control cells in both cell lines).Other activities at the molecular level indicate that functional groups on the prenyl side chain may be important.CONCLUSION:Our findings for the first time demonstrate that four caged xanthones induce apoptosis in CCA cells which is mediated through a mitochondriadependent signaling pathway. | Chariya Hahnvajanawong Wongwarut Boonyanugomol Tapanawan Nasomyon Watcharin Loilome Nisana Namwat Natthinee Anantachoke Wichittra Tassaneeyakul Banchob Sripa Wises Namwat Vichai Reutrakul | 2010 | World Journal of Gastroenterology2010，16，18： | 5 |
| 3 | Downregu-lation of reversion-inducing-cysteine-rich protein with Kazal mo-tifs (RECK) is associated with enhanced expression of matrixmetalloproteinases and cholangiocarcinoma metastases显示文摘 | Namwat N Puetkasichonpasutha J Loilome W | 2011 | J Gas-troenterol2011，46，5： | 1 |
| 4 | Tumor necrosis factor-α modulates epithelial mesenchymal transition mediators ZEB2 and S100A4 to promote cholangiocarcinoma progression显示文摘 | Techasen A Namwat N Loilome W | 2014 | Hepatobiliary Pancreat Sci2014，21，9： | 1 |
| 5 | Identification of the varR gene as a transcriptional regulator of virginiamycin S resistance in Streptomyces virginiae显示文摘 | Namwat W Lee CK Kinoshita H | 2001 | Journal of Bacteriology2001，183，6： | 1 |
| 6 | Down regulation of reversion inducing-cysteine rich protein with Ka zal motifs (RECK) is associated with enhanced expression of matrix metalloproteinases and cholangiocarcinoma metastases 显示文摘 | Namwat N Puetkasichonpasutha J Loilome W | 2011 | J Gastroenterol2011，46，： | 1 |
| 7 | Downregulation of reversion-inducing-cysteine-rich protein with Kasal motifs (RECK) is associated with enhanced expression of matrix metalloproteinases and cholangiocarcinoma metastases 显示文摘 | Namwat N Puetkasichonpasutha J Loilome W | 2011 | J Gastroenterol2011，46，5： | 1 |
| 8 | Identification of the varR gene as a transcriptional regulator of virginiamycin S resistance in Streptomyces virginiae显示文摘 | Namwat W Lee C K Kinoshita H | 2001 | J Bacteriol2001，183，6： | 1 |
| 9 | Vaccination with ALVAC and AIDSVAX to prevent HIV-1 infection in Thailand 显示文摘 | Rerks-Ngarm S Pitisuttithum P Nitayaphan S Kaewkung- wal J Chiu J Paris R Premsri N Namwat C de Souza M Adams E Benenson M Gurunathan S Tartaglia J McNeil JG Francis DP Stablein D Birx DL Chunsuttiwat S Khamboonruang C Thongcharoen P Robb ML MichaelNL Kunasol P Kim JH MOPH-TAVEG Investigators | 2009 | N Engl J Med2009，361，23： | 1 |
| 10 | Downregula- tion of reversion-inducing-cysteine-rich protein with Kazal motifs (RECK) is associated with enhanced expression of matrix显示文摘 | Namwat N Puetkasichonpasutha J Loilone W | 2011 | J Gastroentero!2011，46，5： | 1 |
| 11 | Vaccination with ALVAC and AIDSVAX to prevent HIV-1 infection in Thailand显示文摘 | Rerks-Ngarm S Pitisuttithum P Nitayaphan S Kaewkungwal J Chiu J Paris R Premsri N Namwat C de Souza M Adams E Benenson M Gurunathan S Tartaglia J McNeil JG Francis DP Stablein D Birx DL Chunsuttiwat S Khamboonruang C Thongcharoen P Robb ML Michael NL Kunasol P Kim JH MOPH-TAVEG Investigators | 2009 | N Engl J Med2009，361，23： | 1 |
| 12 | Tumor necrosis factorα (TNF-o) stimulates the epithelial-mesenchymal transition regulator Snail in cholangiocarcinoma显示文摘 | Techasen A Namwat N Loilome W | 2012 | Med Oncol2012，29，5： | 1 |
| 13 | Vaccination with ALVAC and AIDSVAX to prevent HIV-1 infection in Thailand 显示文摘 | Rerks-Ngarm S Pitisuttithum P Nitayaphan S Kaewkung- wal J Chiu J Paris R Premsri N Namwat C de Souza M Adams E Benenson M Gurunathan S Tartaglia J McNeil JG Francis DP Stablein D Birx DL Chunsuttiwat S Khamboonruang C Thongcharoen P Robb ML Michael NL Kunasol P Kim JH MOPHTAVEG Investigators | 2009 | N Engl J Med2009，361，23： | 1 |
| 14 | Establishment and characterization of gemcitabine- resistant human cholangiocarcinoma cell lines with muhidrug resistance and enhanced invasiveness 显示文摘 | Wattanawongdon W Hahnvajanawong C Namwat N | 2015 | Int J Oncol2015，47，1： | 1 |
| 15 | Overexpression of microRNA-21 regulating PDCD4 during tumorigenesis of liver fluke-associated cholangiocarcinoma contributes to tumor growth and metastasis显示文摘 | Chusorn P Namwat N Loilome W | 2013 | Tumour Biol2013，34，3： | 1 |
| 16 | Characterization of 5-fluorouracil-resistant cholangiocarcinoma cell lines 显示文摘 | Namwat N Amimanan P Loilome W etal | 2008 | Chemotherapy2008，54，5： | 1 |
| 17 | The genetic diversity of Mycobacterium tuberculosis strains in Thailand studied by amplification of DNA segments containing direct repetitive sequences 显示文摘 | Luangwuk P Palittapongarnpim P | 1998 | Int J Tuberc Lung dis1998，2，2： | 1 |
| 18 | Upregulation of endothelial nitric oxide synthase(e NOS)and its upstream regulators in Opisthorchis viverrini associated cholangiocarcinoma and its clinical significance显示文摘 | Suksawat M Techasen A Namwat N | 2016 | Parasitol Int2016，30，： | 1 |
| 19 | Application of duplex-PCR in rapid and reliable detection of toxigenic Vibrio cholerae in water samples in Thailand 显示文摘 | Chomvarin C Namwat W Wongwajana S | 2007 | J Gen Appl Microbiol2007，53，4： | 1 |
| 20 | Prevalence of Helicobacter pylori vac A, cag A, cag E, ice A and bab A2 genotypes in Thai dyspeptic patients显示文摘 | Chariya Chomvarin Wises Namwat Kunyaluk Chaicumpar Pisaln Mairiang Apichat Sangchan Banchob Sripa Siripen Tor-Udom Ratha-Khon Vilaichone | 2007 | International Journal of Infectious Diseases2007，，1： | 1 |
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