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| 1 | Roles of the MEK1/2 and AKT pathways in CXCL12/CXCR4 induced cholangiocarcinoma cell invasion显示文摘AIM:To evaluate the expression of C-X-C motif chemokine receptor 4(CXCR4)and its signaling cascades,which were previously identified as a key factor for cancer cell progression and metastasis,in cholangiocarcinoma cell lines.METHODS:The expression of CXCR4 and its signaling cascades were determined in the cholangiocarcinoma cell lines(RMCCA1 and KKU100)by Western blotting.The invasion assays and the detection of actin polymerization were tested in these cholangiocarcinoma cells treated with CXC chemokine ligand-12(CXCL12).RESULTS:Expression of CXCR4 was detected in both cholangiocarcinoma cell lines and activation of CXCR4 with CXCL12 triggered the signaling via the extracellular signal-regulated kinase-1/2(ERK1/2)and phosphoinositide 3-kinase(PI3K)and induction of cholangiocarcinoma cell invasion,and displayed high levels of actin polymerization.Addition of CXCR4 inhibitor(AMD3100)abrogated CXCL12-induced phosphorylation of MEK1/2 and Akt in these cells.Moreover,treatment with MEK1/2 inhibitor(U0126)or PI3K inhibitor(LY294 002)also attenuated the effect of CXCL12-induced cholangiocarcinoma cell invasion.CONCLUSION:These results indicated that the activation of CXCR4 and its signaling pathways(MEK1/2 and Akt)are essential for CXCL12-induced cholangiocarcinoma cell invasion.This rises Implications on a potential role for the inhibition of CXCR4 or its signal cascades in the treatment of cholangiocarcinoma. | Kawin Leelawat Surang Leelawat Siriluck Narong Suradej Hongeng | 2007 | World Journal of Gastroenterology2007，13，10： | 27 |
| 2 | Pre-operative hypoalbuminemia is a major risk factor for postoperative complications following rectal cancer surgery显示文摘AIM:To determine the relationship between pre-operative hypoalbuminemia and the development of complications following rectal cancer surgery, as well as postoperative bowel function and hospital stay. METHODS:The medical records of 244 patients undergoing elective oncological resection for rectal adenocarcinoma at Siriraj Hospital during 2003 and 2006 were reviewed. The patients had pre-operative serum albumin assessment. Albumin less than 35 g/L was recognized as hypoalbuminemia. Postoperative outcomes, including mortality, complications, time to first bowel movement, time to first defecation, time to resumption of normal diet and length of hospital stay, were analyzed. RESULTS:The patients were 139 males (57%) and 105 females (43%) with mean age of 62 years. Fifty-six patients (23%) had hypoalbuminemia. Hypoalbuminemic patients had a significantly larger tumor size and lower body mass index compared with non-hypoalbuminemic patients (5.5 vs 4.3 cm;P < 0.001 and 21.9 vs 23.2 kg/m2;P = 0.02, respectively). Thirty day postoperative mortality was 1.2%. Overall complication rate was 25%. Hypoalbuminemic patients had a significantly higher rate of postoperative complications (37.5% vs 21.3%;P = 0.014). In univariate analysis, hypoalbuminemia and ASA status were two risk factors for postoperative complications. In multivariate analysis, hypoalbuminemia was the only significant risk factor (odds ratio 2.22,95% CI 1.17-4.23;P < 0.015). Hospitalization in hypoalbuminemic patients was significantly longer than that in non-hypoalbuminemic patients (13 vs 10 d, P = 0.034), but the parameters of postoperative bowel function were not significantly different between the two groups. CONCLUSION:Pre-operative hypoalbuminemia is an independent risk factor for postoperative complications following rectal cancer surgery. | Varut Lohsiriwat Darin Lohsiriwat Wiroon Boonnuch Vitoon Chinswangwatanakul Thawatchai Akaraviputh Narong Lert-akayamanee | 2008 | World Journal of Gastroenterology2008，14，8： | 24 |
| 3 | Prospective study of MMP7 serum levels in the diagnosis of cholangiocarcinoma显示文摘AIM: To determine whether the serum level of matrix metalloproteinase-7 (MMP7) has the potential to diagnosis cholangiocarcinoma from benign biliary tract diseases. METHODS: This study was performed according to the PRoBE (a prospective-specimen-collection, retrospectiveblinded-evaluation) design. A total of 187 patients with obstructive jaundice were consecutively enrolled. After the diagnostic status of these patients was ascertained, their levels of serum MMP7 were assayed and compared with serum carbohydrate antigen 19-9 (CA19-9). This was conducted in a blinded case (cholangiocarcinoma)control (benign biliary tract disease) setup. RESULTS: MMP7 and CA19-9 serum levels were significantly elevated in cholangiocarcinoma patients (P < 0.001). The area under the curve (AUC) from a receiver operating characteristic (ROC) curve analysis for thediagnosis of cholangiocarcinoma, using MMP7 was more accurate than CA19-9 (AUC = 0.84, 95% CI: 0.778-0.903 for MMP7 and AUC = 0.79, 95% CI: 0.708-0.868 for CA19-9). The sensitivity and specificity of serum MMP7 (cut-off value of 5.5 ng/mL) was 75% and 78%, respectively, while the sensitivity and specificity of serum CA19-9 (cut-off value of 100 U/mL) was 68% and 87%, respectively. CONCLUSION: Serum values of MMP7 and CA19-9 appear to be useful biomarkers for differentiating cholangiocarcinoma from benign biliary tract obstructive diseases. | Kawin Leelawat Siriluck Narong Jerasak Wannaprasert Thawee Ratanashu-ek | 2010 | World Journal of Gastroenterology2010，16，37： | 7 |
| 4 | Colonoscopic perforation:A report from World Gastroenterology Organization endoscopy training center in Thailand显示文摘瞄准:决定 colonoscopic 穿孔(CP ) 的发生,并且从在泰国训练中心的世界肠胃病学组织(WGO ) 内视镜检查法与 CP 评估病人的临床的调查结果,管理和结果。方法:所有 colonoscopies 和 sigmoidoscopies 在内视镜的单位表现在 1999 和 2007 之间,药 Siriraj 医院的教师, Mahidol 大学,曼谷被考察。CP,病人的特征,内视镜的信息, intra 起作用的调查结果,管理和结果的发生被分析。结果:结肠(13699 colonoscopies 和 3658 灵活 sigmoidoscopies ) 的 17357 内视镜的过程的一个总数在 9 年的经期上在 Siriraj 医院里被执行。十五个病人(0.09%) 有 CP:14 从结肠镜检查并且 1 从乙状结肠镜检查。穿孔的最普通的地点在 S 字形的冒号(80%) ,由横向的冒号(13%) 列在后面。穿孔被直接损伤从柄或内诊镜的尖端引起(n = 12, 80%) 并且内视镜的 polypectomy (n = 3, 20%) 。有 CP 的所有病人经历了外科的管理:主要修理(27%) 和肠切除术(73%) 。死亡率是 13% ,手术后的复杂并发症率是 53% 。结论:CP 是稀罕却严肃的复杂并发症追随者结肠镜检查和乙状结肠镜检查,与病态和死亡的高率。CP 的发生是 0.09% 。外科仍然是 CP 管理的支柱。 | Varut Lohsiriwat Sasithorn Sujarittanakarn Thawatchai Akaraviputh Narong Lertakyamanee Darin Lohsiriwat Udom Kachinthorn | 2008 | World Journal of Gastroenterology2008，14，43： | 6 |
| 5 | Outcome of curative resection for perihilar cholangiocarcinoma in Northeast Thailand显示文摘AIM: To examine survival outcomes of perihilar cholangiocarcinoma(PCCA) resection including mortality, morbidity and prognostic factors. METHODS: Multivariate analyses were carried out based on the survival data of all patients with histologically confirmed PCCA who underwent curative resection at Srinagarind Hospital from January 2006 to December 2011. RESULTS: There were 29(19%) cases of intrahepatic CCA that involved hilar and 124(81%) with hilar bileduct cancer. R0 resection was carried out on 66(43.1%) patients of whom 50(32.7%) also had lymph node metastasis. The other patients underwent R1 resection. The overall 5-year survival rate was 20.6%(95%CI: 13.8-28.4) and median survival time was 19.9 mo. Postoperative mortality was 2%, and 30% of patients had complications. Patients without lymph node metastasis were 60% less likely to die than those with metastasis. Achieving R0 led to a 58% reduction in the chance of mortality as compared to R1. CONCLUSION: To achieve a better survival outcome, focus should center on performing radical surgery and detection of patients with early stage cancer. | Attapol Titapun Ake Pugkhem Vor Luvira Tharatip Srisuk Ongart Somintara O-tur Saeseow Anan Sripanuskul Anongporn Nimboriboonporn Bandit Thinkhamrop Narong Khuntikeo | 2015 | World Journal of Gastrointestinal Oncology2015，7，12： | 5 |
| 6 | Annexin A1: A new immunohistological marker of cholangiocarcinoma显示文摘AIM: To evaluate a new immunohistological marker, annexin A1 (ANXA1), in cholangiocarcinoma (CCA) and hepatocellular carcinoma (HCC). METHODS: Expression of ANXA1 protein was investigated in liver tissues from patients with CCA and HCC by immunohistochemistry. Its expression on differences stages of tumor development was investigated in hamster CCA tissues induced by Opisthorchis viverrini and N -nitrosodimethylamine. Moreover, mRNA expression of ANXA1 was assessed in CCA cell lines by quantitative real-time polymerase chain reaction and silencing of ANXA1 gene expression using small interfering RNA. RESULTS: In human CCA tissue arrays, immunohistochemical analysis revealed that the positive expression of ANXA1 was 94.1% (64/68 cases) consisting of a high expression (66.2%, 45/68 cases) and a low expression (33.8%, 23/68 cases). However, expression of ANXA1 protein was negative in all histologic patterns for HCC (46/46 cases) and healthy individuals (6/6 cases). In hamster with opisthorchiasis-associated CCA, the expression of ANXA1 was observed in the cytoplasm of inflammatory cells, bile duct epithelia and tumor cells. Grading scores of ANXA1 expression were significantly increased with tumor progression. In addition, mRNA expression of ANXA1 significantly increased in all of the various CCA cell lines tested compared to an immortalized human cholangiocyte cell line (MMNK1). Suppressing the ANXA1 gene significantly reduced the matrix metalloproteinase (MMP) 2 and MMP9, and transforming growth factor-β genes, but increased nuclear factor-kB gene expression. CONCLUSION: ANXA1 is highly expressed in CCA, but low in HCC, suggesting it may serve as a new immunohistochemical marker of CCA. ANXA1 may play a role in opisthorchiasis-associated cholangiocarcinogenesis. | Nuttanan Hongsrichan Rucksak Rucksaken Yaovalux Chamgramol Porntip Pinlaor Anchalee Techasen Puangrat Yongvanit Narong Khuntikeo Chawalit Pairojkul Somchai Pinlaorr | 2013 | World Journal of Gastroenterology2013，19，16： | 4 |
| 7 | Prognostic relevance of circulating CK19 mRNA in advanced malignant biliary tract diseases显示文摘AIM: To determine the role of circulating tumor cells (CTCs) in prediction of the overall survival of patients with advanced malignant biliary tract obstruction. METHODS: We investigated the prognostic value of CTCs by examining two markers, cytokeratin (CK) 19 and human telomerase reverse transcriptase (hTERT) mRNA, in 40 patients diagnosed with advanced malig- nant biliary tract diseases. Quantitative real-time re- verse transcription polymerase chain reaction was used to detect CK19 and hTERT mRNA in the peripheral blood of these patients. Overall survival was analyzed using the Kaplan-Meier method and Cox regression modeling.RESULTS: Positive CK19 and hTERT mRNA expression was detected in 45% and 60%, respectively, of the 40 patients. Univariable analysis indicated that positive CK19 mRNA expression was significantly associated with worse overall survival (P = 0.009). Multivariable analysis determined that positive CK19 mRNA expres- sion, patient's age and serum bilirubin were each inde- pendently associated with overall survival. CONCLUSION: CK19 mRNA expression levels in pe- ripheral blood appear to provide a valuable marker to predict the overall survival of patients with advanced malignant biliary tract obstruction. | Kawin Leelawat Siriluck Narong Wandee Udomchaiprasertkul Jerasak Wannaprasert Sa-ard Treepongkaruna Somboon Subwongcharoen Tawee Ratanashu-ek | 2012 | World Journal of Gastroenterology2012，18，2： | 3 |
| 8 | Prognostic factors in resectable cholangiocarcinoma patients: Carcinoembryonic antigen, lymph node, surgical margin and chemotherapy显示文摘AIM: To evaluate outcomes in resectable cholangiocarcinoma patients and to determine prognostic factors. METHODS: A retrospective study was conducted among newly-diagnosed cholangiocarcinoma patients from January 2009 to December 2011 who underwent curative resection in Srinakarind Hospital (a 1000-bed university hospital). Two hundred and sixty-three cholangiocarcinoma patients with good performance were enrolled. These patients had pathological reports with clear margins or microscopic margins. Prognostic factors which included clinical factors, serum liver function test as well as serum tumor makers at presentation,tumor data, and receiving adjuvant chemotherapy were determined by uniand multivariate analysis. RESULTS: The median overall survival time was 17 mo (95%CI: 13.2-20.7); and 1-, 2-, and 3year survival rates were 65.5%, 45.2% and 35.4%. Serum albumin levels, serum carcinoembryonic antigen (CEA) levels, staging classifications by American Joint Committee on cancer, pathological tumor staging, lymph node metastases, tumor grading, surgical margin status, and if adjuvant chemotherapy was administered, were shown to be significant prognostic factors of resectable cholangiocarcinoma by univariate analysis. Multivariate analysis, however, established that only abnormal serum CEA [hazard ratio (HR) 1.68; P = 0.027] and lymph node metastases (HR 2.27; P = 0.007) were significantly associated with a decrease in overall survival, while adjuvant chemotherapy (HR 0.71; P = 0.067) and surgical margin negative (HR 0.72; P = 0.094) tended to improve survival time. CONCLUSION: Serum CEA and lymph node metastases which were associated with advanced stage tumors become strong negative prognostic factors in cholangiocarcinoma. | Kosin Wirasorn Thundon Ngamprasertchai Jarin Chindaprasirt Aumkhae Sookprasert Narong Khantikaew Ake Pakkhem Piti Ungarereevittaya | 2013 | World Journal of Gastrointestinal Oncology2013，5，4： | 3 |
| 9 | Expression of sialyl Lewis^a relates to poor prognosis in cholangiocarcinoma显示文摘AIM: High levels of serum sialyl Lewisa (sLea) are frequently found in cholangiocarinnoma (CCA) patients and have been suggested to be a serum marker for CCA. However, the significance of this antigen in CCA is unknown. In this study,the clinical significance of sLea expression in CCA tissues and the possible role of sLea in vascular invasion in vitro were elucidated.METHODS: Expression of sLea in tumor tissues of 77patients with mass-forming CCA and 33 with periductal infiltrating CCA was determined using immunohistochemistry.The in vitro assays on adhesion and transmigration of CCA cells to human umbilical vein endothelial cells were compared between CCA cell lines with and without sLea expression.RESULTS: sLea was aberrantly expressed in 60% of CCA tumor tissues. A significant relationship was found between the frequency of sLea expression and the mass-forming type CCA (P = 0.041), well differentiated histological grading (P = 0.029), and vascular invasion (P = 0.030). Patients with positive sLea expression had a significantly poorer prognosis (21.28 wk, 95% CI = 16.75-25.81 wk) than those negative for sLea (37.30 wk, 95% CI = 27.03-47.57 wk)(P<0.001). Multivariate analysis with adjustment for all covariates showed that patients positive for sLea possessed a 2.3-fold higher risk of death than patients negative for sLea (P<0.001). The role of sLea in vascular invasion was demonstrated using in vitro adhesion and transmigration assays. KKU-M213, a human CCA cell-line with a high expression of sLea, adhered and transmigrated to IL-1β-activated endothelial cells of the human umbilical vein more than KKU-100, the line without sLea expression (P<0.001).These processes were significantly diminished when the antibodies specific to either sLea or E-selectin were added to the assays (P<0.001).CONCLUSION: This study demonstrates the clinical significance of sLea expression in vascular invasion, and an unfavorable outcome in CCA. The role of sLea in vascular invasion which may lead to poor prognosis is supported by the in vitro adhesion and transmigration studies. | Apa Juntavee Banchob Sripa Ake Pugkhem Narong Khuntikeo Sopit Wongkham | 2005 | World Journal of Gastroenterology2005，11，2： | 2 |
| 10 | Enhanced giant dielectric properties and improved nonlinear electrical response in acceptor-donor (Al^(3+), Ta^(5+))-substituted CaCu_(3)Ti_(4)0_(12) ceramics显示文摘The giant dielectric bchavior of CaCu_(3)Ti_(4)0_(12)(CCTO)has been widely investigated owing to its potential applications in electronics;however,the loss tangent(tan8)of this material is too large for many applications.A partial substitution of CCTO ceramics with either Al^(3+) or Ta^(5+)-ions generally results in poorer nonlinear properties and an associated increase in tan8(to~0.29-1.15).However,first-principles calculations showed that self-charge compensation occurs between these two dopant ions when co-doped into Tit sites,which can improve the electrical properties of the grain boundary(GB).Surprisingly,in this study,a greatly enhanced breakdown electric field(~200--6588 V/cm)and nonlinear coefficient(-4.8-15.2)with a significantly reduced tan8(~0.010--0.036)were obtained by simultaneous partial substitution of CCTO with acceptor-donor(Al^(3+),Ta^(5+))dopants to produce(Al^(3+),Ta^(5+))-CCTO ceramics.The reduced tan8 and improved nonlinear properties were attributed to the synergistic effects of the co-dopants in the doped CCTO structure.The signifcant reduction in the mean grain size of the(Al^(3+),Ta^(5+))-CCTO ceramics compared to pure CCTO was mainly because of the Ta^(5+)-ions.Accordingly,the increased GB density due to the reduced grain size and the larger Schottky barrier height(Ф_(b))at the GBs of the co-doped CCTO ceramics were the main reasons for the greatly increased GB resistance,improved nonlinear properties,and reduced tan8 values compared to pure and single-doped CCTO.In addition,high dielectric constant values(ε'≈(0.52-2.7)×10^(4))were obtained.A fine-grained microstructure with highly insulating GBs was obtained by Ta doping,while co-doping with Ta^(5+) and Al^(3+ )resulted in a high Ф_(b).The obtained results are expected to provide useful guidelines for developing new giant dielectric ceramics with excellent dielectric properties. | Jakkree BOONLAKHORN Narong CHANLEK Jedsada MANYAM Pornjuk SREPUSHARAWOOT Sriprajak KRONGSUK Prasit THONGBAI | 2021 | Journal of Advanced Ceramics2021，10，6： | 2 |
| 11 | Shrimp Taura syndrome virus detection by reverse transcription loop-mediated isothermal amplification combined with a lateral flow dipstick显示文摘 | Wansika Kiatpathomchai Wansadaj Jaroenram Narong Arunrut Sarawut Jitrapakdee T.W. Flegel | 2008 | Journal of Virological Methods2008，，2： | 1 |
| 12 | In- duction of MKP-1 prevents the cytotoxic effects of PI3K inhibition in hilar cholangiocarcinoma cells 显示文摘 | Leelawat K Udomchaiprasertkul W Narong S | 2010 | J Cancer Res Clin Oncol2010，136，10： | 1 |
| 13 | Effect of pH on the ζ-potential and turbidity of yeast suspensions 显示文摘 | NARONG P JAMES A E | 2006 | Colloids and Surfaces A: Pbysicochemical and Engineering Aspects2006，274，： | 1 |
| 14 | Sodium chloride rejection by a UF ceramic membrane in relation to its surface electrical properties显示文摘 | Narong P James A | 2006 | Sep Purif Technol2006，49，： | 1 |
| 15 | Sustained Release of Amoxieillin from Ethyl Cellulose-Coated Amoxieillin Chitosan-Cyclodextrin-Based Tablets 显示文摘 | KULTIDA S JATUPORN P NARONG P | 2010 | AAPS Pharm Sci Teeh2010，12，： | 1 |
| 16 | Quaterly Newsletter of the Pacific Rim 显示文摘 | Narong C C Vetiveria A | 1997 | Vetiver Network1997，1，： | 1 |
| 17 | Effect of the potential on the micro/ ultra - filtration of yeast suspensions using ceramic membranes显示文摘 | Narong P James A E | 2005 | Separation and Purification Technology2005，30，： | 1 |
| 18 | Surgical Outcomes of Lichtenstein Tension-Free Hernioplasty for Acutely Incarcerated Inguinal Hernia显示文摘 | Varut Lohsiriwat Wasupong Sridermma Thawatchai Akaraviputh Wiroon Boonnuch Vitoon Chinsawangwatthanakol Asada Methasate Narong Lert-akyamanee Darin Lohsiriwat | 2007 | Surgery Today2007，，3： | 1 |
| 19 | Rapid and sensitive detection of shrimp infectious myonecrosis virus using a reverse transcription loop-mediated isothermal amplification and visual colorogenic nanogold hybridization probe assay显示文摘 | Narong Arunrut Rungkarn Suebsing Wansika Kiatpathomchai | 2013 | Journal of Virological Methods2013，，： | 1 |
| 20 | Effect of the ζ-potential on the micro/ultrafiltration of yeast suspensions using ceramic membranes显示文摘 | Narong P James A E | 2006 | Separation and Purification Technology2006，49，2： | 1 |
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