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| 1 | In vitro differentiation of human umbilical cord Wharton's jelly mesenchymal stromal cells to insulin producing clusters显示文摘AIM: To investigate the differentiation of human Wharton's jelly derived mesenchymal stromal cells(WJMSCs) to insulin producing clusters(IPC) this study was conducted. METHODS: The umbilical cords samples were collected from full term caesarian section mothers and the WJ-MSCS were cultured from tissue explants in High glucose-Dulbecco's Modified Eagle Medium(H-DMEM); H-DMEM supplemented with 10% fetal bovine serum(FBS) and antibiotics. The expression of CD90, CD44, CD105, CD34 and CD133 as well as osteogenic and adipogenic differentiation of cells in appropriate medium were also evaluated. The cells were differentiated toward IPC with changing the culture medium and adding the small molecules such as nicotinic acid, epidermal growth factor, and exendin-4 during 3 wk period. The gene expression of PDX1, NGN3, Glut2, insulin was monitored by reveres transcription polymerase chain reaction method. The differentiated clusters were stained with Dithizone(DTZ) which confirms the presence of insulin granules. The insulin challenge test(low and high glucose concentration in Krebs-Ringer HEPES buffer) was also used to evaluate the functional properties of differentiated clusters.RESULTS: WJ-MSCS were positive for mesenchymal surface markers(CD90, CD44, CD105), and negative for CD34 and CD133. The accumulation of lipid vacuoles and deposition of calcium mineral in cells were considered as adipogenic and osteogenic potential of WJ-MSCS. The cells also expressed the transcriptional factors such as Nanog and OCT4. During this three step differentiation, the WJ-MSCS morphology was gradually changed from spindle shaped cells in to epithelioid cells and eventually to three dimensional clusters. The clusters expressed PDX1, NGN3, Glut2, and insulin. The cells became bright red color when stained with DTZ and the insulin secretion was also confirmed. In glucose challenge test a significant increase in insulin secretion from 0.91 ± 0.04 μIu/mL(2.8 mmol/L glucose) to to 8.34 ± 0.45 μIu/mL(16.7 mmol/L glucose) was recorded(P < 0.05). The insulin secretion of undifferentiated WJ-MSCS was not changed in this challenge test.CONCLUSION: WJ-MSCs have the ability to differentiate in to islet-like cells in vitro. However, this process needs further optimization in order to generate efficient and functional IPCs. | Seideh Masoomeh Nekoei Negar Azarpira Ladan Sadeghi Sulmaz Kamalifar | 2015 | World Journal of Clinical Cases2015，3，7： | 3 |
| 2 | Comparative study of results and complications of three methods in treatment of scaphoid fractures显示文摘 | Dehghani M Teimouri M Nekoei F | | 0，，109： | 1 |
| 3 | Hospital services quality assess- ment:Hospitals of Kerman University of Medical Sciences, as a tangible example of a developing country显示文摘 | Nekoei MM Amiresmaili M | 2011 | International Journal of Health Care Quality Assurance2011，24，1： | 1 |
| 4 | Current–Voltage Characteristics of the Aziridine-Based Nano-Molecular Wires: a Light-Driven Molecular Switch显示文摘Using nonequilibrium Green's function formalism combined first-principles density functional theory, we analyze the transport properties of a 4,4-dimethyl-6-(4-nitrophenyl)-2-phenyl-3,5-diaza-bicyclo[3.1.0]hex-2-ene molecular optical switch. The title molecule can convert between closed and open forms by visible or ultraviolet irradiation. The I-V characteristics, differential conductance, on-off ratio, electronic transmission coefficients, spatial distribution of molecular projected self-consistent Hamiltonian orbitals, HOMO-LUMO gaps, effect of electrode materials Y(111)(Y =Au, Ag and Pt) on electronic transport and different molecular geometries corresponding to the closed and open forms through the molecular device are discussed in detail. Based on the results, as soon as possible the open form translates to the closed form, and there is a switch from the ON state to the OFF state(low resistance switches to high resistance). Theoretical results show that the donor/acceptor substituent plays an important role in the electronic transport of molecular devices. The switching performance can be improved to some extent through suitable donor and acceptor substituents. | Ayoub Kanaani Mohammad Vakili Davood Ajloo Mehdi Nekoei | 2018 | Chinese Physics Letters2018，35，4： | 0 |
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