| 1 | Effect of N-desulfated heparin on hepatic/renal ischemia reperfusion injury in rats显示文摘AIM: To investigate the effect of N-desulfated heparin onhepatic/renal ischemia and reperfusion injury in rats.METHODS: Using rat models of 60 minutes hepatic or renalischemia followed by 1 h,3 h,6 h and 24 h reperfusion,animalswere randomly divided into following groups,the shamoperated controls,ischemic group receiving only normalsaline,and treated group receiving N-desulfated heparin ata dose of 12 mg/kg at 5 minutes before reperfusion. P-selectin expression was detected in bepatic/renal tissueswith immunohistochemistry methodRESULTS: P-selectin expression, serum ALT, AST, BUN andCr levels were significantly increased during 60 minuteischemia and 1 h, 3 h, 6 h and 24 h reperfusion,while theincrement was significantly inhibited,and hepatic/renalpathology observed by light microscopy was remarkablyimproved by treatment with the N-desulfated heparin.Furthermore,the heparin was found no effects on PT and KPTT.CONCLUSION: P-selectin might mediate neutrophilinfiltration and contribute to hepatic/renal ischemia andreperfusion. The N-desulfated heparin might preventhepatic/renal damage induced by ischemia and reperfusioninjury without significant anticoagulant activity. | Tong Zhou Wei Song Feng Wang Department of Nephrology,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China Jin-Lian Chen Department of Gastroenterology,Shanghai Sixth People’s Hospital,Shanghai 200233,China Ming-Jun Zhang Animal Laboratory,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China Pei-Hua Ni,Department of Biochemistry,Shanghai Second Medical University,Shanghai 200025 Jian-Guo Geng Institute of Biochemistry and Cell Biology,Shanghai 200030,China | 2002 | World Journal of Gastroenterology2002，8，5： | 20 |
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