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| 1 | Fatty acid content in epididymal fluid and spermatozoa during sperm maturation in dogs显示文摘Background: During sperm maturation, there is a reorganization of fatty acids from plasmatic membrane of the spermatozoa, which allows higher membrane integrity and acquisition of sperm motility. However, the fatty acid profile during sperm maturation remains unclear in dogs. Thus, the aim of this study was to identify the fatty acids from the epididymal spermatozoa and plasma during the sperm maturation, and observed changes in the motility and plasmatic membrane parameters. Twenty one adult dogs were used, subsequently to bilateral orchiectomy and epididymal storage, sperm samples were collected from the different segments of the epididymis. Samples were evaluated for conventional microscopy, computer-assisted motility analysis, sperm plasma membrane permeability and the fatty acid analysis(lipids were extracted, transmethylated and analyzed by chromatography).Results: Caput and corpus sperm showed lower values for the motility variables evaluated and plasmatic membrane integrity, indicating different levels of the fatty acids organization. Saturated, monounsaturated and polyunsaturated fatty acids were in higher concentrations in the spermatozoa from epididymis cauda. Highlighting the presence of caprylic, stearic and docosahexaenoic acids.Conclusions: These findings demonstrate the influence of the fatty acid profile during sperm maturation, assigning physical and chemical changes in sperm cells, essential for fertilization. | Daniel S.Ramos Angrimani Marcilio Nichi Joāo Diego A.Losano Cristina F.Lucio Gisele A.Lima Veiga Márcia V.M.Junqueira Franco Camila I.Vannucchi | 2017 | Journal of Animal Science and Biotechnology2017，8，3： | 3 |
| 2 | Five level triage= a report from the ACEP/ENA five - level triage task force显示文摘 | Christopher M Paula T Nichi G | 2005 | J Emer- gency Nuts2005，31，： | 1 |
| 3 | Poor ovarian re- sponse in patients younger than 35 years: is it also a qualita- tive decline in ovarian function? 显示文摘 | Figueira Rde C Braga DP Nichi M | 2009 | Hum Fertil (Camb)2009，12，3： | 1 |
| 4 | Poor ovarian response in patients younger than 35 years: is it also a qualitative decline in ovarian function? 显示文摘 | Figueira RC Braga DP Nichi M | 2009 | Hum Fertil (Camb)2009，12，3： | 1 |
| 5 | A comparison of post-thaw results between embryos arising from intracytoplasmic sperm injection using surgically retrieved or ejaculated spermatozoa显示文摘 | DE CSSIA SAVIO FIGUEIRA R MADASCHI C NICHI M | 2009 | Fertil Steril2009，91，3： | 1 |
| 6 | Poor ovarian response in patients younger than 35 years: is it also a qualitative decline in ovarian function显示文摘 | Figueira Rde C Braga DP Nichi M | 2009 | Hum Fertil2009，12，： | 1 |
| 7 | Poor ovarian response in patients younger than 35 years: is it also a qualitative decline in ovarian function显示文摘 | FIGUEIRA RDE C BRAGA DP NICHI M | 2009 | Hum Fertil (Camb)2009，12，3： | 1 |
| 8 | Lagrangian Relaxation with Cut Generation for Hybrid Flow Shop Scheduling Problems to Minimize the Total Weighted Tardiness显示文摘 | NICHI T HIRANAKA Y INUIGUCHI M | 2010 | Computers & Operations Research2010，37，1： | 1 |
| 9 | Poor ovarian response in patients younger than 35 years: Is it also a qualitative decline in ovarian function? 显示文摘 | Figueira RD Braga DP Nichi M | 2009 | Hum Fertil(Camb)2009，18，6： | 1 |
| 10 | Poor ovarian response in patients younger than 35 years:is it also a qualitative decline in ovarian function显示文摘 | Figueira Rde C Braga D P Nichi M | 2009 | Hum Fertil2009，12，3： | 1 |
| 11 | Poor ovarian response in patients younger than 35 years: Is it also a qualitative decline in ovarian function? 显示文摘 | Figueira Rde C Braga DP Nichi M | 2009 | Hum Fertil (Camb)2009，12，3： | 1 |
| 12 | Poor ovarian response in patients younger than 35 years:is it also a qualitative decline in ovarian function显示文摘 | Figueira Rde C Braga DP Nichi M | 2009 | Hum Fertil(Camb)2009，12，： | 1 |
| 13 | New molecular vari ants of hypothalamus-pituitary-gonad axis genes and their associ- ation with early puberty phenotype in Bos taurus indicus (Nel- lore)显示文摘 | Milazzotto M P Rahal P Nichi M | 2008 | Livestock Science2008，114，2： | 1 |
| 14 | Sex-sorted sperm for artifi- cial insemination and embryo transfer programs in cattle 显示文摘 | Filho M S Nichi M Soares J G | 2014 | Official Journal of the Brazilian College of Animal Reproduction2014，11，3： | 1 |
| 15 | Poor ovarian response in pa-tients younger Than 35 years:is it also a qualitative decline in o-varian function显示文摘 | Figueira R Braga DP?Nichi M | 2009 | Hum Fertil (Camb)2009，12，16： | 1 |
| 16 | A comparison of post-thaw results between embryos arising from intracytoplasmic sperm injection using surgically retrieved or ejaculated spermatozoa 显示文摘 | de Cassia Savio Figueira R Madaschi C Nichi M | 2009 | Fertil Steril2009，91，3： | 1 |
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