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| 1 | Vascularization and osteogenesis in ectopically implanted bone tissue-engineered constructs with endothelial and osteogenic differentiated adipose-derived stem cells显示文摘BACKGROUND A major problem in the healing of bone defects is insufficient or absent blood supply within the defect.To overcome this challenging problem,a plethora of approaches within bone tissue engineering have been developed recently.Bearing in mind that the interplay of various diffusible factors released by endothelial cells(ECs)and osteoblasts(OBs)have a pivotal role in bone growth and regeneration and that adjacent ECs and OBs also communicate directly through gap junctions,we set the focus on the simultaneous application of these cell types together with platelet-rich plasma(PRP)as a growth factor reservoir within ectopic bone tissue engineering constructs.AIM To vascularize and examine osteogenesis in bone tissue engineering constructs enriched with PRP and adipose-derived stem cells(ASCs)induced into ECs and OBs.METHODS ASCs isolated from adipose tissue,induced in vitro into ECs,OBs or just expanded were used for implant construction as followed:BPEO,endothelial and osteogenic differentiated ASCs with PRP and bone mineral matrix;BPUI,uninduced ASCs with PRP and bone mineral matrix;BC(control),only bone mineral matrix.At 1,2,4 and 8 wk after subcutaneous implantation in mice,implants were extracted and endothelial-related and bone-related gene expression were analyzed,while histological analyses were performed after 2 and 8 wk.RESULTS The percentage of vascularization was significantly higher in BC compared to BPUI and BPEO constructs 2 and 8 wk after implantation.BC had the lowest endothelial-related gene expression,weaker osteocalcin immunoexpression and Spp1 expression compared to BPUI and BPEO.Endothelial-related gene expression and osteocalcin immunoexpression were higher in BPUI compared to BC and BPEO.BPEO had a higher percentage of vascularization compared to BPUI and the highest CD31 immunoexpression among examined constructs.Except Vwf,endothelial-related gene expression in BPEO had a later onset and was upregulated and well-balanced during in vivo incubation that induced late onset of Spp1 expression and pronounced osteocalcin immunoexpression at 2 and 8 wk.Tissue regression was noticed in BPEO constructs after 8 wk.CONCLUSION Ectopically implanted BPEO constructs had a favorable impact on vascularization and osteogenesis,but tissue regression imposed the need for discovering a more optimal EC/OB ratio prior to considerations for clinical applications. | Jelena G Najdanović Vladimir J Cvetković Sanja T Stojanović MarijaĐVukelić-Nikolić Jelena MŽivković Stevo J Najman | 2021 | World Journal of Stem Cells2021，13，1： | 3 |
| 2 | Biliary peri tonitis caused by a leaking T-tube fistula disconnected at the point of contact with the anterior abdominal wall: a case report显示文摘 | Nikoli M Karthikesalingam A Nachimuthu S | 2008 | J Med Case Reports2008，2，9： | 1 |
| 3 | Position Control of an Electro-hydraulic Servo System Using Sliding Mode Control Enhanced by Fuzzy PI Controller 显示文摘 | Mihajlov M Nikolie V Antie D | 2002 | Mechanical Engineering2002，1，9： | 1 |
| 4 | Transit network design by Bee Colony Optimization显示文摘 | Nikoli? M Teodorovi? D | 2013 | Expert Systems with Applications2013，40，15： | 1 |
| 5 | Deconvoluted Fourier- transform LNT-IR study of coordination copper (1I) compounds with dextran derivatives显示文摘 | NIKOLI~ G S CAKI(~ M MITI~ ~ | 2008 | Russian Journal of Coordination Chemistry2008，34，5： | 1 |
| 6 | Molecular modeling of wine polyphenols显示文摘 | Rastija V Nikoli'c S Medi'c-Sari'c M | 2009 | J Math Chem2009，，46： | 1 |
| 7 | Vitamin D deficiency is associated with Increased IL-17 and TNF-α levels in patients with chronic heart failure显示文摘 | Milovanovic M Pesic G Nikolie V | 2012 | Arq Bras Cardiol2012，98，3： | 1 |
| 8 | The PARACHUTE IV trial design and rationale: percutaneous ventricular restoration using the parachute device in patients with ischemic heart failure and dilated left ventricles显示文摘 | Costa MA Pencina M Nikolie S | 2013 | Am Heart J2013，165，： | 1 |
| 9 | On the Zagreb indices as complexity indices显示文摘 | Nikoliic S Tolic I M Trinajstic N | | 0，，： | 1 |
| 10 | Significance of the spatial reconstruction based on mathematical modeling in the surgical trearment of giant intracranial aneurysms显示文摘 | Nikoli IM Naguli M Anmnovic V | 2006 | Vojnosanit Pregl2006，63，1： | 1 |
| 11 | Transit network design by bee colony optimization显示文摘 | Nikoli? M Teodorovi? D | 2013 | Expert Systems with Applications2013，40，15： | 1 |
| 12 | New materials for solar thermal storage-solid liquid transitions in fatty acid esters显示文摘 | Nikoli? R Marinovi?-Cincovi? M Gad?uri? S | 2003 | Solar Energy Materials & Solar Cells2003，79，3： | 1 |
| 13 | Determination of spinning force on ring spinning machine显示文摘 | Nikolie M | 1975 | Tekstil 241975，，： | 1 |
| 14 | Dynamic modelling of hydraulic excavator motion using Kane's equations显示文摘 | Salinie S Boakovie G Nikolie M | 2014 | Automation in Con- struction2014，44，： | 1 |
| 15 | Hydrogen absorption and electrochemical properties of Mg2Ni-type alloys synthesized by mechanical alloying显示文摘 | SIMI(C)I(C) M V ZDUJI(C) M DIMITRIJEVI(C) R NIKOLI(C)-BUJANOVI(C) LJ POPOVI(C) N H | 2006 | J Power Sources2006，158，： | 1 |
| 16 | Effect of buckwheat flour addition to wheat flour on acylglycerols and fatty acids composition and theology properties显示文摘 | Nikolie N Sakac M Mastilovic J | 2011 | Food Science and Technology2011，44，： | 1 |
| 17 | Photoluminescence of europium doped Li In O2 powder显示文摘 | ?A?ANIN L R DRAMI?ANIN M D NIKOLI?M G | 2011 | Phys Status Solidi2011，8，28： | 1 |
| 18 | Sleep spindle altera- tions in patients with parkinsong disease显示文摘 | Christensen JA Nikolie M Warby SC | 2015 | Frontiers in Human Neuroseienee2015，9，： | 1 |
| 19 | Conversion of p35 to p25 deregulates Cdk5 activity and promotes neurodegeneration显示文摘 | Patrick GN Zukerberg L Nikolie M | 1999 | Nature1999，402，6762： | 1 |
| 20 | Changes in the astrocytic aquaporin-4 and inwardly rectifying potassium channel expression in the brain of the amyotrophic lateral sclerosis SOD1(G93A) rat model显示文摘 | Batavelji(c) D Nikoli(c) L Milosevi(c) M Todorovi(c) N Andjus PR | | 0，，12： | 1 |
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