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| 1 | Dual-action penems and carbapenems显示文摘 | Alfred J C Scott L D Norma K D | 1992 | J Med Chem1992，35，10： | 1 |
| 2 | Lactational Performance after Breast Reduction with Different Pedicles显示文摘 | Norma IC Leo K | 2007 | Plast Reconstr Surg2007，120，： | 1 |
| 3 | HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium显示文摘BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence factor.AIM To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGS) cells.METHODS Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGS cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPASy platform, and using the GLYCOPP platform for glycosylation sites. Different programs, via, I-TASSER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional modeling.RESULTS HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium cells.Immunogold technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGS cells.Bioinformatics analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein to the external environment. The programs also showed a similarity with the Tip-alpha protein of H. pylori. Tip-alpha is an exotoxin that penetrates cells and induces tumor necrosis factor alpha production, and HP0953 could have a similar function as posttranslational modification sites were found;modifications in turn require enzymes located in eukaryotic cells. Thus, to be functional, HP0953 may necessarily need to be translocated inside the cell where it can trigger different mechanisms producing cellular damage.CONCLUSION The location of HP0953 around infected cells, the probable posttranslational modifications, and its similarity to an exotoxin suggest that this protein is a virulence factor. | Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio Alma L Olivares-Cervantes Juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo Sandra Mendoza-Elizalde Edgar O López-Villegas Christian Colín Pamela Aguilar-Rodea Alfonso Reyes-López Marcela Salazar García Norma Velázquez-Guadarrama | 2022 | World Journal of Gastroenterology2022，28，29： | 1 |
| 4 | The impact of consumer involvement on satisfaction with and use ofassistive technology显示文摘 | Martin Jay K Martin Liam G Stumbo Norma J | 2010 | Disability and Rehabilitation: Assistive Technology2010，6，3： | 1 |
| 5 | Serum dioxin, testosterone, and subsequent risk of benign prostatic hyperplasia : a prospective cohort study of air force veterans显示文摘 | Amit G Norma K Claus G R | 2006 | Environ Health Perspect2006，114，11： | 1 |
| 6 | Viruses and virus like particles detected during examination of feces from calves and piglets with diarrhea显示文摘 | Durham P J K Hassard L E Norma G R B | 1989 | Can Vet J1989，30，11： | 1 |
| 7 | Total parathy- roidectomy under local anaesthesia for renal hyperparathyroidism 显示文摘 | CHEONG Y T TAIB N A NORMA YAH K | 2009 | Asian J Surg2009，32，1： | 1 |
| 8 | 显示文摘 | De T Norma R Rajeshwar K | 2002 | Electrochim Acta2002，16，： | 1 |
| 9 | An intervention to increase quality of life and self - care self - efficacy and decrease symptoms in breast cancer patients 显示文摘 | Lev E L Daley K M Norma E C | 2001 | Scholarly Inquiry for Nursing Practice2001，15，3： | 1 |
| 10 | Emerging therapeutic options in inflammatory bowel disease显示文摘Inflammatory bowel disease(IBD)is a chronic disease that requires chronic treatment throughout the evolution of the disease,with a complex physiopathology that entails great challenges for the development of new and specific treatments for ulcerative colitis and Crohn's disease.The anti-tumor necrosis factor alpha therapy has impacted the clinical course of IBD in those patients who do not respond to conventional treatment,so there is a need to develop new therapies and markers of treatment response.Various pathways involved in the development of the disease are known and the new therapies have focused on blocking the inflammatory process at the gastrointestinal level by oral,intravenous,subcutaneous,and topical route.All these new therapies can lead to more personalized treatments with higher success rates and fewer relapses.These treatments have not only focused on clinical remission,but also on achieving macroscopic changes at the endoscopic level and microscopic changes by achieving mucosal healing.These treatments are mainly based on modifying signaling pathways,by blocking receptors or ligands,reducing cell migration and maintaining the integrity of the epithelial barrier.Therefore,this review presents the efficacy and safety of the new treatments that are currently under study and the advances that have been made in this area in recent years. | Jesus K Yamamoto-Furusho Norma N Parra-Holguín | 2021 | World Journal of Gastroenterology2021，27，48： | 0 |
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