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| 1 | Lack of correlation between Treg quantification assays in inflammatory bowel disease patients显示文摘AIM:To compare the number of regulatory T-cells( Tregs) measured by flow cytometry with those obtained using a real-time quantitative PCR(q PCR) method in patients suffering from inflammatory bowel disease(IBD).METHODS:Tregs percentages obtained by both flow cytometry and q PCR methods in 35 adult IBD patients,18 out of them with Crohn′s disease(CD)and 17 with ulcerative colitis(UC)were compared to each other as well as to scores on two IBD activity questionnaires using the Harvey Bradshaw Index(HBI)for CD patients and the Simple Colitis Clinical Activity Index(SCCAI)for UC patients.The Treg percentages by flow cytometry were defined as CD4+CD25highCD127lowFOXP3+cells in peripheral blood mononuclear cells,whereas the Treg percentages by q PCR method were determined as FOXP3 promoter demethylation in genomic DNA.RESULTS:We found an average of 1.56%±0.78%Tregs by using flow cytometry,compared to 1.07%±0.53%Tregs by using q PCR in adult IBD patients.There were no significant correlations between either the percentages of Tregs measured by flow cytometry or q PCR and the HBI or SCCAI questionnaire scores in CD or UC patients,respectively.In addition,there was no correlation between Treg percentages measured by q PCR and those measured by flow cytometry(r=-0.06,P=0.73;Spearman Rho).These data suggest that,either Treg-related immune function or the clinical scores in these IBD patients did not accurately reflect actual disease activity.Until the cause(s)for these differences are more clearly defined,the resultssuggest caution in interpreting studies of Tregs in various inflammatory disorders.CONCLUSION:The two methods did not produce equivalent measures of the percentage of total Tregs in the IBD patients studied which is consistent with the conclusion that Tregs subtypes are not equally detected by these two assays. | Gunnar Brandhorst Darinka Todorova Petrova Sebastian Weigand Christoph Eberle Nicolas von Ahsen Jessica Schmitz Frank Christian Schultze Dirk Raddatz Michael Karaus Michael Oellerich Philip D Walson | 2015 | World Journal of Gastroenterology2015，21，11： | 2 |
| 2 | Two-hour cyclosporing concentration determination: an appropriate tool to monitor neoral therapy显示文摘 | Oellerich M Armstrong VW | 2002 | Ther Drug Monit2002，24，1： | 1 |
| 3 | Rapid detection of ITPA 94C>A and IVS2+21A>C gene mutations by real-time fluorescence PCR and in vitro demonstration of effect of ITPA IVS2+21A>C polymorphism on splicing efficiency显示文摘 | Oellerich M Armstrong VW | 2004 | Clin Chem2004，50，11： | 1 |
| 4 | Therapeutic drug monitoring of cyclosporine and tacrolimus显示文摘 | Oellerich M Armstrong VW Schutz E | 1998 | Clin Biochem1998，31，5： | 1 |
| 5 | Induction of inducible nitric oxide synthase is an essential part of tumor necrosis factor -alpha- induced apoptosis in MCF - 7 mad other epithelial tumor cells显示文摘 | Binder C Schulz M Hiddemann W Oellerich M | 1999 | Lab Invest1999，79，： | 1 |
| 6 | Immunosuppressive drug monitoring of sirolimus and cyclosporine in pediatric patients显示文摘 | Oellerich M Armstrong V W Streit F | 2004 | Clini Biochemist2004，37，6： | 1 |
| 7 | Characterization of diabetic nephropathy by urinary proteomic analysis: identification of a processed ubiquitin form as a differentially excreted protein in diabetic nephropathy patients 显示文摘 | Dihazi H Mtiller GA Lindner S Meyer M Asif AR Oellerich M | 2007 | Clin Chem2007，53，9： | 1 |
| 8 | The role of therapeutic drug monitoring in individualizing immunosuppressive drug therapy:Recent developments 显示文摘 | Oellerich M Armstrong VW | 2006 | Therapeutic Drug Monitoring2006，28，6： | 1 |
| 9 | Current issues in therapeutic drug monitoring of mycophenolic acid: Report of a roundtable discussion 显示文摘 | SHAW L M HOLT D W OELLERICH M | 2001 | Ther Drug Monit2001，23，4： | 1 |
| 10 | IDEBENONE PROTECTS HEPATIC MICROSOMES AGAINST OXYGEN RADICAL-MEDIATED DAMAGE IN ORGAN PRESERVATION SOLUTIONS显示文摘 | EBERHARD WIELAND EKKEHARD SCHüTZ VICTOR W. ARMSTRONG FRIEDHELM KüTHE CHRISTIAN HELLER MICHAEL OELLERICH | 1995 | Transplantation1995，，： | 1 |
| 11 | Measurement of erythrocyte inosine triphosphate pyrophosphohydrolase (ITPA) activity by HPLC and correlation of ITPA geno- type-phenotype in a caucasian population显示文摘 | Shipkova M Lorenz K Oellerich M | 2006 | Clin Chem2006，52，2： | 1 |
| 12 | Rapid liquid chromatography-tandem mass spectrometry routine method for simultaneous determination of sirolimus, everolimus, tacrohmus, and cyclosporin A in whole blood 显示文摘 | Streit F Armstromg VW Oellerich M | 2002 | Clin Chem2002，48，61： | 1 |
| 13 | Differential proteome analysis of human embryonic kidney cell line ( HEK- 293) following mycophenolic acid treatment 显示文摘 | QASIM M RAHMAN H OELLERICH M | 2011 | Proteome Sci2011，57，9： | 1 |
| 14 | Current issues in therapeutic drug monitoring of mycophenolic acid: reportof a roundtable discussion显示文摘 | Shaw LM Holt DW Oellerich M | 2001 | Ther Drug Monit2001，23，4： | 1 |
| 15 | β2 inte- grin-derived signals induce cell survival and prolifera tion of AML blasts by activating a SYK/STAT signa- ling axis显示文摘 | Oellerich T Oellerich M F Engelke M | 2013 | Blood2013，121，19： | 1 |
| 16 | Two-hour cyclosporine concentration determination:an appropriate tool to monitor neoral therapy显示文摘 | Oellerich M Armstrong V W | 2002 | Ther Drug Monit2002，24，1： | 1 |
| 17 | Lidocaine Metabolite Formation as a Measure of Liver Function in Patients with Cirrhosis显示文摘 | OELLERICH M BRUDELSKI M LAUTZ H U | 1990 | Ther Drug Monit1990，12，3： | 1 |
| 18 | Rapid liquid chromatography-tandem mass spectrometry routine method for simultaneous determination of sirolimus, everolimus, tacrolimus, and cyclosporin A in whole blood显示文摘 | STREIT F ARMSTRONG V W OELLERICH M | 2002 | Clin Chem2002，48，： | 1 |
| 19 | No influence of the MDR-1 C3435T polymorphism or a CYP3A4 promoter polymorphism (CYP3A4-V co allele) on doseadjusted eyclosporin Atrough ncentrations or rejection incidence in stable renal transplant recipients 显示文摘 | von Ahsen N Richter M Grupp C Ringe B Oellerich M Armstrong VW | 2001 | Clin Chem2001，47，6： | 1 |
| 20 | Immunosuppressive drug monitoring of sirolimus and cyclosporine in pediatric patients 显示文摘 | OELLERICH M ARMSTRONG V W STREIT F | 2004 | Clin Biochem2004，37，6： | 1 |
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