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| 1 | A silybin-phospholipids complex counteracts rat fatty liver degeneration and mitochondrial oxidative changes显示文摘AIM:To investigate the effectiveness of antioxidant compounds in modulating mitochondrial oxidative alterations and lipids accumulation in fatty hepatocytes.METHODS:Silybin-phospholipid complex containing vitamin E(Realsil) was daily administered by gavage(one pouch diluted in 3 mL of water and containing 15 mg vitamin E and 47 mg silybin complexed with phospholipids) to rats fed a choline-deprived(CD) or a high fat diet [20% fat,containing 71% total calories as fat,11% as carbohydrate,and 18% as protein,high fat diet(HFD)] for 30 d and 60 d,respectively.The control group was fed a normal semi-purified diet containing adequate levels of choline(35% total calories as fat,47% as carbohydrate,and 18% as protein).Circulating and hepatic redox active and nitrogen regulating molecules(thioredoxin,glutathione,glutathione peroxidase),NO metabolites(nitrosothiols,nitrotyrosine),lipid peroxides [malondialdehyde-thiobarbituric(MDA-TBA)],and pro-inflammatory keratins(K-18) were measured on days 0,7,14,30,and 60.Mitochondrial respiratory chain proteins and the extent of hepatic fatty infiltration were evaluated.RESULTS:Both diet regimens produced liver steatosis(50% and 25% of liver slices with CD and HFD,respectively) with no signs of necro-inflammation:fat infiltration ranged from large droplets at day 14 to disseminated and confluent vacuoles resulting in microvesicular steatosis at day 30(CD) and day 60(HFD).In plasma,thioredoxin and nitrosothiols were not significantly changed,while MDA-TBA,nitrotyrosine(from 6 ± 1 nmol/L to 14 ± 3 nmol/L day 30 CD,P < 0.001,and 12 ± 2 nmol/L day 60 HFD,P < 0.001),and K-18(from 198 ± 20 to 289 ± 21 U/L day 30 CD,P < 0.001,and 242 ± 23 U/L day 60 HFD,P < 0.001) levels increased significantly with ongoing steatosis.In the liver,glutathione was decreased(from 34.0 ± 1.3 to 25.3 ± 1.2 nmol/mg prot day 30 CD,P < 0.001,and 22.4 ± 2.4 nmol/mg prot day 60 HFD,P < 0.001),while thioredoxin and glutathione peroxidase were initially increased and then decreased.Nitrosothiols were constantly increased.MDA-TBA levels were five-fold increased from 9.1 ± 1.2 nmol/g to 75.6 ± 5.4 nmol/g on day 30,P < 0.001(CD) and doubled with HFD on day 60.Realsil administration significantly lowered the extent of fat infiltration,maintained liver glutathione levels during the first half period,and halved its decrease during the second half.Also,Realsil modulated thioredoxin changes and the production of NO derivatives and significantly lowered MDA-TBA levels both in liver(from 73.6 ± 5.4 to 57.2 ± 6.3 nmol/g day 30 CD,P < 0.01 and from 27.3 ± 2.1 nmol/g to 20.5 ± 2.2 nmol/g day 60 HFD,P < 0.01) and in plasma.Changes in mitochondrial respiratory complexes were also attenuated by Realsil in HFD rats with a major protective effect on Complex Ⅱ subunit CII-30.CONCLUSION:Realsil administration effectively contrasts hepatocyte fat deposition,NO derivatives formation,and mitochondrial alterations,allowing the liver to maintain a better glutathione and thioredoxin antioxidant activity. | Ignazio Grattagliano Catia V Diogo Maria Mastrodonato Ornella de Bari Michele Persichella David QH Wang Adriana Liquori Domenico Ferri Maria Rosaria Carratù Paulo J Oliveira Piero Portincasa | 2013 | World Journal of Gastroenterology2013，19，20： | 6 |
| 2 | Genetic ancestry analysis in non-alcoholic fatty liver disease patients from Brazil and Portugal显示文摘AIM:To study the association between genetic ancestry,non-alcoholic fatty liver disease(NAFLD) metabolic characteristics in two cohorts of patients,from Brazil and Portugal. METHODS:We included 131 subjects from Brazil [(n = 45 with simple steatosis(S. Steatosis) and n = 86 with nonalcoholic steatohepatitis(NASH)] and 90 patients from Portugal(n = 66,S. Steatosis; n = 24,NASH). All patients had biopsy-proven NAFLD. In histologic evaluation NAFLD activity score was used to assess histology and more than 5 points defined NASH in this study. Patients were divided into two groups according to histology diagnosis:simple steatosis or non-alcoholic statohepatitis. Genetic ancestry was assessed using real-time polymerase chain reaction. Seven ancestry informative markers(AT3-I/D,LPL,Sb19.3,APO,FYNull,PV92,and CKMM) with the greatest ethnicgeographical differential frequencies(≥ 48%) were used to define genetic ancestry. Data were analyzed using R PROJECTS software. Ancestry allele frequencies between groups were analyzed by GENEPOP onlineand the estimation of genetic ancestry contribution was evaluated by ADMIX-95 software. The 5% alpha-error was considered as significant(P < 0.05). RESULTS:In the Brazilian sample,NASH was significantly more frequent among the elderly patients with diabetes(NASH 56 ± 1.1 years old vs S. Steatosis 51 ± 1.5 years old,P = 3.7 x 10-9),dyslipidemia(NASH 63% vs S. Steatosis 37%,P = 0.009),higher fasting glucose levels(NASH 124 ± 5.2 vs S. Steatosis 106 ± 5.3,P = 0.001) and Homeostatic Model of Assessment index > 2.5 [NASH 5.3(70.8%) vs S. Steatosis 4.6(29.2%) P = 0.04]. In the Portuguese study population,dyslipidemia was present in all patients with NASH(P = 0.03) and hypertension was present in a larger percentage of subjects in the S. Steatosis group(P = 0.003,respectively). The genetic ancestry contribution among Brazilian and Portuguese individuals with NASH was similar to those with S. Steatosis from each cohort(Brazilian cohort:P = 0.75; Portuguese cohort:P = 0.97). Nonetheless,the genetic ancestry contribution of the Brazilian and Portuguese population were different,and a greater European and Amerindian ancestry contribution was detected in the Portuguese population while a higher African genetic ancestry contribution was observed in Brazilian population of both NASH and S. Steatosis groups.CONCLUSION:There was no difference between the genetic ancestry contribution among Brazilian and Portuguese individuals with NASH and S. Steatosis from each cohort. | Lourianne Nascimento Cavalcante Jose Tadeu Stefano Mariana V Machado Daniel F Mazo Fabiola Rabelo Kiyoko Abe Sandes Flair Jose Carrilho Helena Cortez-Pinto Andre Castro Lyra Claudia P de Oliveira | 2015 | World Journal of Hepatology2015，7，10： | 2 |
| 3 | A multiobjeetive design of a patient and anaesthetist-friendly neuromuscular blockade controller显示文摘 | FAZENDEIRO P OLIVEIRA J V D PEDRYCZ W | 2007 | IEEE Transactions on Biomedical Engineering2007，54，9： | 1 |
| 4 | A Control-volume based Finite Element Method for Three-dimensional Incompressible Turbulent Fluid Flow, Heat Transfer and Related Phenomena 显示文摘 | Costa V A F Oliveira L A Figueiredo A R | 1995 | Int J Numer Mesh Fluid1995，21，5： | 1 |
| 5 | Antioxi- dant and antiglyeation properties of Passiflora alata and Passiflora edulis extracts显示文摘 | Rudnicki M Oliveira M R Pereira T V | 2007 | Food Chemistry2007，100，： | 1 |
| 6 | Reproductive biology of crocodile shark Pseudocarcharias kamoharai 显示文摘 | OLIVEIRA P HAZIN F H V CARVALHO F | 2010 | Journal of Fish Biology2010，76，7： | 1 |
| 7 | Enzymatic alcoholysis of palm kernel oil in n-hexane and SCCO2 显示文摘 | OLIVEIRA D OLIVEIRA V | 2001 | SupercritIca I Fluids2001，19，： | 1 |
| 8 | Ampicillin/sulbactam compared with polymyxins for the treatment of infections caused by carbapenem-resistant Acinetobacter spp显示文摘 | Oliveira M S Prado G V Costa S F | 2008 | J Antimicrob Chemother2008，61，： | 1 |
| 9 | A model for PEG removal from alumina injection moulded parts by solvent debinding显示文摘 | Oliveira A A M Klein A N | 2007 | Journal of Material Processing Technology2007，182，： | 1 |
| 10 | Observing the evolution of Intemet as topology 显示文摘 | Oliveira R V Zhang B Zhang L | 2007 | SIGCOMM Comput Commun Rev2007，37，4： | 1 |
| 11 | Residual stress analysis in Co-based laser clad layers by laboratory X-rays and synchrotron diffraction techniques 显示文摘 | U De Oliveira V Ocelik J Th M De Hosson | 2006 | Surface & Coatings Technology2006，201，34： | 1 |
| 12 | A Proposed Mechanism for Nitrate and Thiocyanate Elution of Strong-base Ion Exchange Resins Loaded with Copper and Gold Cyanocomplexes 显示文摘 | Oliveira A M Leao V A Silva C A da | 2008 | React Funct Polym2008，68，1： | 1 |
| 13 | Inflammation and poor response to treatment with erythropoie- tin in chronic kidney disease显示文摘 | OLIVEIRA JUNIOR W'V SABINO AP F1GUEIREDO RC | 2015 | J Bras Ncfrol2015，37，2： | 1 |
| 14 | Gamma surgery for melanoma metastases in the brain显示文摘 | MINGIONE V OLIVEIRA M PRASAD D et el | 2002 | Neurosurg2002，96，3： | 1 |
| 15 | Determination ofammonium in marine waters using a gas diffusion multicommutedflow injection system with in-line prevention of metal hydroxidesprecipitation显示文摘 | Oliveira S M Lopes T Toth I V | 2009 | J Environ Monit2009，11，22： | 1 |
| 16 | History,current status,and collaborative research projects for Bemisia tabaci显示文摘 | Oliveira M R V Henneberry T J Anderson P | | 0，，9： | 1 |
| 17 | Multipl-exed fiber Bragg grating interrogation system using a modulated fiber bragg grating and the Tunable-Filter method显示文摘 | PATERNO A S OLIVEIRA V D E | 2006 | IEEE Sensors Journal2006，6，6： | 1 |
| 18 | New evidences of antimalarial activity of Bidens pilosa roots extract correlated with polyacetylene and flavonoids显示文摘 | Oliveira F Q Andrade-Neto V Krettli A U | 2004 | J Ethnopharmacol2004，93，1： | 1 |
| 19 | As- sessment of avrami, ozawa and avrami-ozawa equations for determination of EVA crosslinking kinetics from DSC meas- urements 显示文摘 | BIANCHI O OLIVEIRA R V B FIORIO R | 2008 | Polym Test2008，27，6： | 1 |
| 20 | Role of plant lipid transfer proteins in plant cell physiology-A concise review显示文摘 | Oliveira Carvalho A O Gomes V M | 2007 | Peptides2007，28，： | 1 |
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