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1RGF1 INSENSITIVE 1 to 5, a group of LRR receptor-like kinases, are essential for the perception of root meristem growth factor I in Arabidopsis thaliana显示文摘RGF1,分泌的肽荷尔蒙,戏在 Arabidopsis 在根分裂组织开发给角色调音。以前的研究显示功能的 RGF1 需要是在由 tyrosylprotein sulfotransferase 的酷氨酸残余的 sulfated 并且 RGF1 主要经由二个下游的抄写因素调整根分裂组织活动,过多 1 (PLT1 ) 并且 PLT2。然而,细胞外的 RGF1 怎么被一个植物房间察觉,是不清楚的。用基因途径,我们发现了充满白氨酸的重复的 clade 像受体的 kinases,指定了为 RGF1 感觉迟钝 1 (RGI1 ) 到 RGI5,用作 RGF1 的受体。二独立 rgi1 rgi2 rgi3 rgi4 rgi5 五倍的异种与分裂组织的一种小尺寸显示一致的短主要的根显型。四倍的异种显示出的 rgi1 rgi2 rgi3 rgi4 到 RGF1 的显著地减少的敏感,和五倍的异种对 RGF1 完全感觉迟钝。PLT1 和 PLT2 的表示在五倍的异种是几乎无法发现的。一个 RGI2 倡导者极大地在五倍的异种驾驶的 PLT2 的宫外的表示救了它的根分裂组织缺点。RGI 之一, RGI1,随后详细生物化学地被分析。在 vitro 点弄污和下拉,分析显示 RGI1 能身体上与 RGF1 交往。RGF1 的外长的申请能快速并且同时导致 RGI1 的 phosphorylation 和 ubiquitination,显示 RGI1 能察觉并且 transduce RGF1 肽信号。然而,激活的 RGI1 多半很快被翻。这些结果表明那 RGI,充当 RGF1 的受体,在在 Arabidopsis thaliana 的 RGF1-PLT-mediated 根分裂组织开发的戏必需品角色。Yang Ou Xiaoting Lu Quaner Zi Qingqing Xun Jingjie Zhang Yujun Wu Hongyong Shi Zhuoyun Wei Baolin Zhao Xiaoyue Zhang Kai He Xiaoping Gou Chuanyou Li Jia Li 2016Cell Research2016,26,6:18
2Sand-mediated ice seeding enables serum-free low-cryoprotectant cryopreservation of human induced pluripotent stem cells显示文摘Human induced pluripotent stem cells(hiPSCs)possess tremendous potential for tissue regeneration and banking hiPSCs by cryopreservation for their ready availability is crucial to their widespread use.However,contemporary methods for hiPSC cryopreservation are associated with both limited cell survival and high concentration of toxic cryoprotectants and/or serum.The latter may cause spontaneous differentiation and/or introduce xenogeneic factors,which may compromise the quality of hiPSCs.Here,sand from nature is discovered to be capable of seeding ice above10◦C,which enables cryopreservation of hiPSCs with no serum,much-reduced cryoprotectant,and high cell survival.Furthermore,the cryopreserved hiPSCs retain high pluripotency and functions judged by their pluripotency marker expression,cell cycle analysis,and capability of differentiation into the three germ layers.This unique sand-mediated cryopreservation method may greatly facilitate the convenient and ready availability of high-quality hiPSCs and probably many other types of cells/tissues for the emerging cell-based translational medicine.Bin Jiang Weijie Li Samantha Stewart Wenquan Ou Baolin Liu Pierre Comizzoli Xiaoming He 2021Bioactive Materials2021,6,12:1
3Optical propagation through non-Kolmogorov turbulence显示文摘In this paper,the effects of the generalized exponent,the height and the zenith angle on the log-amplitude variance in the weak fluctuation are investigated.The theoretical results indicate that for the downlink,the log-amplitude variance of the Kolmogorov model is always smaller than that of the three-layer model,while for the uplink,there is a point of intersection in the log-amplitude variance curves of the two models.The different phenomena for the downlink and uplink are analyzed in detail.Further,we find a method to ascertain precise values of the boundary layer altitudes for the three-layer model under various atmospheric conditions through the analysis for the point of intersection.And at the point of intersection,the Kolmogorov model can be used to replace the three-layer model to simplify the analysis of the system performance.Moreover,the log-amplitude variance increases with the increase of the zenith angle.TANG Hua OU BaoLin 2013Science China(Information Sciences)2013,56,4:0
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