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| 1 | Fluid bed pyrolysis of an hydride hardened epoxy resins and polyester-polyurethane by the hamburg process显示文摘 | Grittner N Kaminsky W Obst G | 1993 | J Anal Appl Pyrolysis1993，25，： | 1 |
| 2 | Changes in the racial composition of Phytophthora sojae in Australia between 1979 and 1996显示文摘 | Ryley M J Obst N R Irwin J A G | 1998 | Plant Dis1998，82，： | 1 |
| 3 | In vivo application of RNA leads to induction of specific cytotoxic T lymphocytes and antibodies 显示文摘 | Hoerr I Obst R Rammensee H G | 2000 | Eur J Immunol2000，30，1： | 1 |
| 4 | Chemical solution deposition(CSD) of CeO2 and LZO buffer layers on cube textured NiW substrates 显示文摘 | KOTZYBA G OBST B NAST R el al | 2006 | Appl Super2006，43，： | 1 |
| 5 | MALDI- TOF mass spectrometry-multivariate data analysis as a tool for classification of reactivation and non-eulturable states of bacteria 显示文摘 | Kuehl B Marten SM Bischoff Y Brenner-Weiss G Obst U | 2011 | Anal Bioanal Chem2011，401，5： | 1 |
| 6 | Changes in the racial composition of Phytophthora sojae in Australia between 1979 and 1996 显示文摘 | Ryley M J Obst N R Irwin J A G | 1998 | Plant Disease1998，82，： | 1 |
| 7 | Chemical Solution Deposition (CSD) of CeO2 and La2Zr2O7 Buffer Layers on Cube Textured NiW Substrates 显示文摘 | Kotzybal G Obst B Nast R | 2006 | Journal of Physics : Conference Series2006，43，： | 1 |
| 8 | Changes in the racial composition of Phytophthora sojae in Australia between 1979 and 1996 显示文摘 | Ryley M J Obst N R Irwin J A G | 1998 | Plant Disease1998，82，： | 1 |
| 9 | Changes in the racial com- position of Phytophthora sojae in Australia between 1979 and 1996 显示文摘 | Ryley M J Obst N R Irwin J A G | 1998 | Plant Disease1998，82，： | 1 |
| 10 | Osteocyte specific responses to soluble and mechanical stimuli in a stem cell derived culture model 显示文摘 | Thompson WR Uzer G B~'obst KE | 2015 | Sci Rep2015，5，11： | 1 |
| 11 | Changes in the racial composition of Phytophthora sojae in Australia between 1979 and 1996 显示文摘 | Ryley M J Obst N R Irwin J A G | 1998 | Plant Disease1998，82，： | 1 |
| 12 | The activity of rifampin and analogs against Staphylococcus epidermidis biofilms in a CAPD environment model 显示文摘 | Obst G Gagnon RF Harris A | 1989 | Am J Nephrol1989，9，5： | 1 |
| 13 | An Exploration of Sense of Community, Part3: Dimensions and Predictors of Psychological Sense of Community in Geographical Commtmities显示文摘 | Obst P Smith S G &Zinkiewicz L | 2002 | Journal of Community Psychology2002，30，1： | 1 |
| 14 | Sense of Community in Science Fiction Fandom, Part 2: Comparing Neighborhood and Interest Group Sense of Community显示文摘 | Obst P Zinkiewicz L Smith S G | 2002 | Journal of Community Psychology2002，30，1： | 1 |
| 15 | Changes in the racial composition of Phytophthora sojae in Australia between 1979 and 1996显示文摘 | Ryley M J Obst N R Irwin J A G | 1998 | Plant Dis1998，82，： | 1 |
| 16 | The activity of ri- fampin and analogs against Staphylococcus epidermidisb- iofilm in a CAPD environment model显示文摘 | Obst G Gagnon RF Harris A | 1989 | Am J Nephrol1989，9，： | 1 |
| 17 | Chemical solution deposition(CSD) of CeO2 and LZO buffer layers on cube textured NiW substrates 显示文摘 | KOTZYBA G OBST B NAST R | 2006 | Appl Super2006，43，： | 1 |
| 18 | The Pichia pastoris enzyme production platform:From combinatorial library screening to bench-top fermentation on residual cyanobacterial biomass显示文摘The demand for industrial enzymes is continually rising,fueled by the growing need to shift towards more sustainable industrial processes.However,making efficient enzyme production strains and identifying optimal enzyme expression conditions remains a challenge.Moreover,the production of the enzymes themselves comes with unavoidable impacts,e.g.,the need to utilize secondary feedstocks.Here,we take a more holistic view of bioprocess development and report an integrative approach that allows us to rapidly identify improved enzyme expression and secretion conditions and make use of cyanobacterial waste biomass as feed for supporting Pichia pastoris fermentation.We demonstrate these capabilities by producing a phytase secreted by P.pastoris that is grown on cyanobacterium hydrolysate and buffered glycerol-complex(BMGY)medium,with genetic expression conditions identified by high-throughput screening of a randomized se-cretion library.When our best-performing strain is grown in a fed-batch fermentation on BMGY,we reach over 7000 U/mL in three days. | Korbinian Sinzinger Ulrike Obst Samed Güner Manuel Döring Magdalena Haslbeck Doris Schieder Volker Sieber | 2024 | Journal of Bioresources and Bioproducts2024，9，1： | 0 |
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