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| 1 | CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C virus-coinfected patients显示文摘AIM To characterize peripheral blood natural killer(NK) cells phenotypes by flow cytometry as potential biomarker of liver fibrosis in human immunodeficiency virus(HIV)/hepatitis C virus(HCV) coinfected patients.METHODS Peripheral mononuclear cells from 24 HIV/HCV(HBVnegative) coinfected and 5 HIV/HCV/HBV seronegative individuals were evaluated. HIV/HCV coinfected patients were divided in to groups: G1, patients with METAVIR F0-F2 and G2, patients with METAVIR F3-F4. NK surface cell staining was performed with: AntiCD3(APC/Cy7), anti-CD56(PE/Cy5), anti-CD57(APC), anti-CD25(PE), anti-CD69(FITC), anti-NKp30(PE), antiNKp46(PE/Cy7), anti-NKG2D(APC), anti-DNAM(FITC); anti-CD62L(PE/Cy7), anti-CCR7(PE), anti-TRAIL(PE), anti-Fas L(PE), anti CD94(FITC). Flow cytometry data acquisition was performed on BD FACSCanto, analyzed using Flow Jo software. Frequency of fluorescence was analyzed for all single markers. Clinical records were reviewed, and epidemiological and clinical data were obtained.RESULTS Samples from 11 patients were included in G1 and from 13 in G2. All patients were on ARV, with undetectable HIV viral load. Liver fibrosis was evaluated by transient elastography in 90% of the patients and with biopsy in 10% of the patients. Mean HCV viral load was(6.18 ± 0.7 log10). Even though, no major significant differences were observed between G1 and G2 regarding NK surface markers, it was found that patients with higher liver fibrosis presented statistically lower percentage of NK cells than individual with low to mild fibrosis and healthy controls(G2: 5.4% ± 2.3%, G1: 12.6% ± 8.2%, P = 0.002 and healthy controls 12.2% ± 2.7%, P = 0.008). It was also found that individuals with higher liver fibrosis presented lower CD4 LT count than those from G1(G2: 521 ± 312 cells/μL, G1: 770 ± 205 cells/μL; P = 0.035).CONCLUSION Higher levels of liver fibrosis were associated with lower percentage of NK cells and LTCD4+ count; and they may serve as noninvasive biomarkers of liver damage. | Natalia Laufer Diego Ojeda María Laura Polo Ana Martinez Héctor Pérez Gabriela Turk Pedro Cahn Norberto Walter Zwirner Jorge Quarleri | 2017 | World Journal of Hepatology2017，9，25： | 2 |
| 2 | Verbal fluency in schizophrenia: dose cognitive performance reflect the same under显示文摘 | Ojeda N Scinchez P Pena J | 2010 | J Nerv Ment Dis2010，198，4： | 1 |
| 3 | Verbal fluency inschizophrenia : does cognitive performance reflect the sameunderlying mechanisms in patients and healthy controls显示文摘 | Ojeda P Sdnchez P Peha J | 2010 | J Nerv Ment Dis2010，198，4： | 1 |
| 4 | Benige cutaneous Degos disease 显示文摘 | Ojeda Cuchillero RM Sanchez Regana M Umbert Millet P | 2003 | Clin Exp Dermatol2003，28，： | 1 |
| 5 | Sorafenib in combination with capecitabine: an oral regimen for patients with HER2-negative locally advanced or metastatic breast cancer 显示文摘 | Baselga J Segalla JG Roch6 H Del Giglio A Pinczowski H Ciruelos EM Filho SC G6mez P Van Eyll B Bermejo B Llombart A Garicochea B Durdn Md Hoff PM Espi6 M de Moraes AA Ribeiro RA Mathias C Gil Gil M Ojeda B Morales J Kwon Ro S Li S Costa F | 2012 | J Clin Oncol2012，30，13： | 1 |
| 6 | LS-SVMlab toolbox user’s guide 显示文摘 | De Brabanter K Karsmakers P Ojeda F | 2011 | ESAT-SISTATechnical Report2011，，： | 1 |
| 7 | Transesterification of triglycerides by CaO:Increase of the reaction rate by biodiesel addition显示文摘 | LOPEZ GRANADOS M MARTIN ALONSO D ALBA-RUBIO A C MARISCAL R OJEDA M BRETTERS P | | 0，，04： | 1 |
| 8 | Guild structure of carnivorousintertidal fishes of the Chilean coast:implications ofontogenetic dietary shifts显示文摘 | Munoz A A Ojeda F P | 1998 | Oecologia1998，114，4： | 1 |
| 9 | Chronic microinflammation and endo- thelialdamage in uremia 显示文摘 | Ojeda R Aljama P A | 2008 | Nefrologla2008，28，6： | 1 |
| 10 | Desertificationin central Argentina: Changes in Environment Carbonand Nitrogen from Imaging Spectroscopy 显示文摘 | ASNER G P BORHI C E OJEDA R A | 2003 | Ecologi-cal Applications2003，13，3： | 1 |
| 11 | intrinsic neurons in the rat ovary:an immunohistochemical study显示文摘 | D'albora H lombide P Ojeda SR | 2000 | Cell Tissue Res2000，300，1： | 1 |
| 12 | Guild structure of carnivorous intertidal fishes in the Chilean eoast:implications of ontogenetic dietary shifts显示文摘 | MUNOZ A A OJEDA F P | 1998 | Oecologia1998，，114： | 1 |
| 13 | Effect of water depth on the removal of organic matter in horizontal subsurface flow constructed wetlands 显示文摘 | AGUIRRE P OJEDA E GARCIA J | 2005 | Journal of Environmental Science and Health Part A - Toxic/Hazardous Substances & Environmental Engineering2005，40，67： | 1 |
| 14 | Verbal fluency in schizophrenia: does cognitive performance reflect the same underlying mechanisms in patients and healthy controls显示文摘 | Ojeda N Sanchez P Pea J | 2010 | J Nerv Ment Dis2010，198，4： | 1 |
| 15 | Hydrogenation of carbon oxides over promoted Fe-Mn catalysts prepared by the microemulsion methodology显示文摘 | HERRANZ T ROJAS S PEREZ-ALONSO F J OJEDA M TERRERDS P FIERRO J L G | 2006 | Appl Catal A2006，311，： | 1 |
| 16 | Verbal fluency in schizophrenia: does cognitive performance reflect the same underlying mechanisms in patients and healthy controls 显示文摘 | Ojeda N Snanchez P Pena J | 2010 | J NervMent Dis2010，198，4： | 1 |
| 17 | Verbal fluency in schizophreni- a:does cognitive performance reflect the same underlying mecha- nisms in patients and healthy controls 显示文摘 | Ojeda N Sanchez P Peoa J | 2010 | J New Ment Dis2010，198，： | 1 |
| 18 | Modeling starch gelatinization kinetics of milled rice flour显示文摘 | Carlos A ojeda Marcela P Tolaba Constantino Suarez | 2000 | Cereal Chemistry2000，77，2： | 1 |
| 19 | Guild structure of carnivorous intertidal fishes of the Chilean coast: implications of ontogenetic dietary shifts 显示文摘 | MUNOZ A A OJEDA F P | 1998 | Oecologia1998，114，4： | 1 |
| 20 | Flourescence self-quenching of the molecular forms of Rhodamine B in aqueous and ethanolic solutions 显示文摘 | Arbeloa F L Ojeda P R Arbeloa I L | 1989 | Journal of luminescence1989，44，： | 1 |
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