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| 1 | Pregnancies established through intracytoplasmic sperm injection (ICSI) using spermatozoa with dysplasia of fibrous sheath显示文摘Aim: Dysplasia of the fibrous sheath (DFS) is an anomaly found in asthenozoospermic patients with extremely low or absent motility. In order to determine the efficacy of ICSI in these patients, a retrospective analysis of ICSI results in DFS patients has been done. Methods: Ten ICSI attempts were performed in 6 patients with diagnosis of Dysplasia of the Fibrous Sheath studied by transmission and scanning electron microscopy. Results: In the cases studied, sperm concentration was (29.62 _+ 18.05) x 106/mL, total motility was 1.14 _+ 1.31%. Progressive motility was 0% except for one case with 0.1%. One hundred and three preovulatory oocytes were obtained and 94 metaphase 11 oocytes were injected. Sixty-nine of them showed two pronuclei (fertilization rate: 73.4% ). Forty-nine embryos were obtained and 34 were transferred (mean: 3.4 embryos per transfer). Five pregnancies were diagnosed by β-hCG plasma level determinations that resulted to be one preclinical abortion, one clinical abortion and three deliveries. Another pregnancy (ongoing) was achieved from a cryopreserved embryo transfer. Conclusion: These results showed that ICSI provides a suitable solution for patients suffering from irreversible sperm defects such as DFS. Nevertheless, it is mandatory to inform couples of possible transmission risks to offspring, which are unknown at present. Only when the etiology of this problem is disclosed, it will be possible to assess the real genetic risk. | Santiago Brugo Olmedo Vanesa Y.Rawe Florencia N.Nodar German D.Galavema Anibal A.Acosta Hector E.Chemes | 2000 | Asian Journal of Andrology2000，2，2： | 7 |
| 2 | Clinical-pathological and molecular characterization of long-term survivors with advanced non-small cell lung cancer显示文摘Objective:Long-term survivors(LS)of non-small cell lung cancer(NSCLC)without driver alterations,displaying an overall survival(OS)of more than 3 years,comprise around 10%of cases in several series treated with chemotherapy.There are classical prognosis factors for these cases[stage,Eastern Cooperative Oncology Group(ECOG),etc.],but more data are required in the literature.In this multi-center study,we focused on LS of advanced NSCLC with OS above 36 months to perform a clinical-pathological and molecular characterization.Methods:In the first step,we conducted a clinical-pathological characterization of the patients.Afterwards,we carried out a genetic analysis by comparing LS to a sample of short-term survivors(SS)(with an OS less than 9 months).We initially used whole-genome RNA-seq to identify differentiating profiles of LS and SS,and later confirmed these with reverse transcription-polymerase chain reaction(RT-PCR)for the rest of the samples.Results:A total of 94 patients were included,who were mainly men,former smokers,having adenocarcinoma(AC)-type NSCLC with an ECOG of 0-1.We obtained an initial differential transcriptome expression,displaying 5 over-and 33 under-expressed genes involved in different pathways:namely,the secretin receptor,surfactant protein,trefoil factor 1(T FF1),serpin,Ca-channels,and Tolllike receptor(TLRs)families.Finally,RT-PCR analysis of 40(20 LS/20 SS)samples confirmed that four genes(surfactant proteins and SFTP)were significantly down-regulated in SS compared to LS by using an analysis of covariance(ANCOVA)model:SFTPA1(P=0.023),SFTPA2(P=0.027),SFTPB{P=0.02),and SFT PC(P=0.047).Conclusions:We present a sequential genetic analysis of a sample of NSCLCLS with no driver alterations,obtaining a differential RNA-seq/RT-PCR profile showing an abnormal expression of SF genes. | Juan Moreno-Rubio Santiago Ponce Rosa Alvarez Maria Eugenia Olmedo Sandra Falagan Xabier Mielgo Fatima Navarro Patricia Cruz Luis Cabezon-Gutierrez Carlos Aguado Gonzalo Colmenarejo Marta Munoz-Fernandez de Leglaria Ana Belen Enguita Maria Cebollero Amparo Benito Isabel Alemany Carolina del Castillo Ricardo Ramos Ana Ramirez de Molina Enrique Casado Maria Sereno | 2020 | Cancer Biology & Medicine2020，17，2： | 4 |
| 3 | New horizons for uncommon mutations in non-small cell lung cancer: BRAF, KRAS, RET, MET, NTRK, HER2显示文摘The 2004 discovery of EGFR mutations,followed by ALK rearrangements,ushered in a targeted therapy era for advanced non-small cell lung cancer(NSCLC).Tyrosine kinase inhibitors targeting gene alterations have substantially improved survival and quality of life for patients with NSCLC.In the last decade,rearrangements of the ROS1 oncogene have been incorporated into healthcare practice that are applicable to another small subgroup of patients who benefit from similar targeted strategies.Recent genome studies of lung adenocarcinoma have identified other possible therapeutic targets,including RET,NTRK fusions,c-MET alterations,and activating mutations in KRAS,BRAF,and HER2,all with frequencies greater than 1%.Lung cancers harbouring these genome changes can potentially be treated with agents approved for other indications or under clinical development.This review updates the therapeutic arsenal that especially targets those genes. | Maria Eugenia Olmedo Raquel Cervera Luis Cabezon-Gutierrez Yolanda Lage Elena Corral de la Fuente Ana Gomez Rueda1,Xabier Mielgo-Rubio Juan Carlos Trujillo Felipe Counago | 2022 | World Journal of Clinical Oncology2022，13，4： | 3 |
| 4 | Treatment of chronic hepatitis C with direct-acting antivirals: The role of resistance显示文摘The use of direct-acting antivirals(DAAs) to treat chronic hepatitis C has resulted in a significant increase in rates of sustained viral response(around 90%-95%) as compared with the standard treatment of peginterferon/ribavirin. Despite this, however, the rates of therapeutic failure in daily clinical practice range from 10%-15%. Most of these cases are due to the presence of resistant viral variants, resulting from mutations produced by substitutions of amino acids in the viral target protein that reduce viral sensitivity to DAAs, thus limiting the efficacy of these drugs. The high genetic diversity of hepatitis C virus has resulted in the existence of resistance-associated variants(RAVs), sometimes even before starting treatment with DAAs, though generally at low levels. These preexisting RAVs do not appear to impact on the sustained viral response, whereas those that appear after DAA therapy could well be determinant in virological failure with future treatments. As well as the presence of RAVs, virological failure to treatment with DAAs is generally associated with other factors related with a poor response, such as the degree of fibrosis, the response to previous therapy, the viral load or the viral genotype. Nonetheless, viral breakthrough and relapse can still occur in the absence of detectable RAVs and after the use of highly effective DAAs, so that the true clinical impact of the presence of RAVs in therapeutic failure remains to be determined. | Miguel Jiménez-Pérez Rocío González-Grande Pilar Espana Contreras Isabel Pinazo Martínez Jesús de la Cruz Lombardo Raúl Olmedo Martín | 2016 | World Journal of Gastroenterology2016，22，29： | 3 |
| 5 | Histomorphometric study of bone healing around laminar implants in experimental diabetes显示文摘 | Giglio MJ Giannunzio G Olmedo D | 2000 | Implant Dent2000，9，2： | 1 |
| 6 | Chemical compositionand sensory analysis of roasted peanuts coated with prickly pear andalgarrobo pod syrups显示文摘 | NePote V Mestrallet MG’Olmedo RH | 2008 | Grasasy Aceites2008，59，2： | 1 |
| 7 | Optical coherence tomo- graphy for the characterization of hasal cell carcinoma in vivo : a pilot study显示文摘 | Olmedo JM Warschaw KE Schnfitt JM | 2006 | J Am Acad Dermatol2006，55，3： | 1 |
| 8 | Mocrowave Characterization and Grannlar Materials显示文摘 | OLMEDO L CHATEAU G DELEUZE C | 1993 | Journal of Applied Physics1993，73，10： | 1 |
| 9 | Effect of titanium dioxide on the oxidative metabolism of alveolar macrophages : an experimental study in rats 显示文摘 | OLMEDO D G TASAT D R GUGLIELMOTTI M B | 2005 | J Biomed Mater Res A2005，73，2： | 1 |
| 10 | 显示文摘 | Olmedo L Chateau G Deleuze C | 1993 | J Appl Phys1993，73，10： | 1 |
| 11 | Determination of toxic elements (mercury, cadmium, lead, tin and arsenic) in fish and shellfish samples. Risk assessment for the consumers显示文摘 | P. Olmedo A. Pla A.F. Hernández F. Barbier L. Ayouni F. Gil | 2013 | Environment International2013，，： | 1 |
| 12 | Cytokeletal organization defects and abortive activation in human oocytes after IVF and ICSI failure显示文摘 | Rawe V Y Olmedo S B Nodar F N | 2000 | Mol Hum Reprod2000，6，6： | 1 |
| 13 | Growth, under laboratory conditions of turbot, Scophthalmus maximus, from the Ria de Vigo (North-west Spain) 显示文摘 | LGLESIAS J OLMEDO M OTERO J J | 1978 | Mar Biol1978，96，： | 1 |
| 14 | A program to protocolize nursing care 显示文摘 | Olmedo Luceren MC Sanchidrien de Bias C P16 Mestre R | 2010 | Rev Cali- dAsist2010，25，5： | 1 |
| 15 | Resistive switching in sin- gle epitaxial ZnO nanoislands 显示文摘 | Qi J Olmedo M Ren J J | 2012 | ACS Nano2012，6，2： | 1 |
| 16 | Effect of Platelet - Rich Plasma on the Peri - implant Bone Response: An Experimental Study 显示文摘 | Fontana S Olmedo DG Linares JA | 2004 | IMPLANT DENTISTRY2004，13，1： | 1 |
| 17 | Microwave characterization and modelization of magnetic granular materials显示文摘 | CHATEAU G DELECUZE C | 1993 | J Appl Phys1993，，10： | 1 |
| 18 | Modification of sexual development and carotene production by acetate and other small carboxylic acids in Blakeslea trispora and Phycomyces blakesleeanus 显示文摘 | Vera Kuzina Enrique Cerda- Olmedo | 2006 | Appled and Environmental Microbiology2006，72，： | 1 |
| 19 | Ultrastructural pathology of the sperm flagellum:Association between flagellar pathology and fertility prognosis in severely asthenozoospermic men显示文摘 | Chemes HE Olmedo SB Carrere C | | 0，，9： | 1 |
| 20 | On the prediction of bolted single-lap composite joints显示文摘 | álvaro Olmedo Carlos Santiuste | 2012 | Composite Structures2012，，6： | 1 |
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