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| 1 | Modulation of protein tyrosine phosphorylation in gastric mucosa during re-epithelization processes显示文摘AIM:To investigate the role of protein tyrosine phosphorylation in gastric wound formation and repair following ulceration. METHODS:Gastric lesions were induced in rats using restraint cold stress.To investigate the effect of oxidative and nitrosative cell stress on tyrosine phosphorylation during wound repair,total activity of protein tyrosine kinase(PTK),protein tyrosine phosphatase (PTP),antioxidant enzymes,nitric oxide synthase (NOS), 2',5'-oligoadenylate synthetase,hydroxyl radical and zinc levels were assayed in parallel. RESULTS:Ulcer provocation induced an immediate decrease in tyrosine kinase(40% in plasma membranes and 56% in cytosol,(P<0.05) and phosphatase activity (threefold in plasma membranes and 3.3-fold in cytosol),followed by 2.3-2.4-fold decrease (P<0.05) in protein phosphotyrosine content in the gastric mucosa. Ulceration induced no immediate change in superoxide dismutase (SOD) activity,30% increase (P<0.05) in catalase activity,2.3-fold inhibition (P<0.05) of glutathione peroxidase,3.3-fold increase (P<0.05) in hydroxyl radical content,and 2.3-fold decrease (P<0.05) in zinc level in gastric mucosa.NOS activity was three times higher in gastric mucosa cells after cold stress. Following ulceration,PTK activity increased in plasma membranes and reached a maximum on day 4 after stress (twofold increase,P<0.05),but remained inhibited(1.6-3-fold decrease on days 3,4 and 5,P<0.05) in the cytosol.Tyrosine phosphatases remained inhibited both in membranes and cytosol(1.5-2.4-fold,P< 0.05).NOS activity remained increased on days 1,2 and 3(3.8-,2.6-,2.2-fold,respectively,P<0.05).Activity of SOD increased 1.6 times(P<0.05)days 4 and 5 after stress.Catalase activity normalized after day 2. Glutathione peroxidase activity and zinc level decreased (3.3-and 2-fold,respectively,P<0.05)on the last day. Activity of 2',5'-oligoadenylate synthethase increased 2.8-fold (P<0.05) at the beginning,and 1.6-2.3-fold (P<0.05) during ulcer recuperation,and normalized on day 5,consistent with slowing of inflammation processes. CONCLUSION:These studies show diverse changes in total tyrosine kinase activity in gastric mucosa during the recovery process.Oxidative and nitrosative stress during lesion formation might lead to the observed reduction in tyrosine phosphorylation during ulceration. | Olena V Bogdanova Larysa I Kot Kateryna V Lavrova Volodymyr B Bogdanov Erica K Sloan Tetyana V Beregova Ludmyla I Ostapchenko | 2010 | World Journal of Biological Chemistry2010，1，11： | 2 |
| 2 | Overexpression and refolding of thioredoxin TRAIL fusion from in- clusion bodies and further purification of TRAIL after cleavage by enteropeptidase 显示文摘 | GASPARIAN M E OSTAPCHENKO V G YAGOLOVICH A V | 2007 | Biotechnology Letters2007，29，10： | 1 |
| 3 | Overexpression and refolding of thioredoxin/TRAIL fusion from inclusion bodies and further purification of TRAIL after cleavage by enteropeptidase显示文摘 | Gasparian M E Ostapchenko V G Yagolovich A V | 2007 | Biotechnology Letters2007，29，10： | 1 |
| 4 | Expression,purification,and characterization of human enteropeptidase catalytic subunit in Escherichia coli显示文摘 | Gasparian ME Ostapchenko VG Schulga AA Dolgikh DA Kirpichnikov MP | | 0，，： | 1 |
| 5 | QGSJET-II: Results for extensive air showers 显示文摘 | OSTAPCHENKO S | 2006 | Nuclear Physics B-Proceedings Supplements2006，151，1： | 1 |
| 6 | Expression, purification, and characterization of human enteropeptidase catalytic subunit in Escherichia coli 显示文摘 | Gasparian M E Ostapchenko V G Schulga A A | 2003 | Protein Expr Purif2003，31，1： | 1 |
| 7 | The transient receptor potential melastatin 2(TRPM2)channel contributes toβ-amyloid oligomer-related neurotoxicity and memory impairment显示文摘 | Ostapchenko VG Chen M Guzman MS Xie YF Lavine N Fan J | 2015 | J Neurosci2015，35，15： | 1 |
| 8 | Two amyloid states of the prion protein dis-play significantly different folding patterns显示文摘 | Ostapchenko | 2010 | J Mol Biol2010，400，90： | 1 |
| 9 | Expression, purification, and characterization of human enteropeptidase catalytic subunit in Escherichia coli显示文摘 | Gasparian ME Ostapchenko VG Schulga AA | 2003 | Protein Expression and Purification2003，31，1： | 1 |
| 10 | Biochemical Characterization of Human Enteropeptidase Light Chain显示文摘 | Gasparian ME Ostapchenko VG Dolgikh DA | | 0，，02： | 1 |
| 11 | Models for RHIC and LHC: New Devdopments 显示文摘 | Werner K Drescher H J Ostapchenko S | 2002 | Nucl Phys A2002，698，： | 1 |
| 12 | Interferon-induced 2', 5'- oligoadenylate system: key components and biological functions 显示文摘 | MYKK HAILYK I V OSTAPCHENKO L I Kucher- enko M I | 2003 | Ukr Biokhim Zh2003，75，3： | 1 |
| 13 | Bionanocomposite Effect on Mucosal Protection Indicators at Mucosa Gastric Ulceration Simulation显示文摘 | Yakubtsova Irina Volodymynvna Khilko Tatiana Dmytrivna Voronin Evgeny Pylypovych PreobrazhenskaTamara Dmytrivna Ostapchenko Lyudmila Ivanivna Makai Sandor | 2013 | 材料科学与工程(中英文A版)2013，3，9： | 1 |
| 14 | Lipid Composition of Blood and Liver Cells under Consumption Trigonella Foenum Graecum L, on the Background High-Calorie Diet显示文摘 | Khilko Tatiana Dmytrivna Yakubtsova Irina Volodymyrivna Konopelnyuk Victoria Vasylivna PreobrazhenskaTamara Dmytrivna Kot Larisa Ivanivna Ostapchenko Lyudmila Ivanivna | 2016 | 材料科学与工程(中英文A版)2016，6，2： | 0 |
| 15 | Impact of Bionanocomposite on Some Immunological and Biochemical Parameters at Gastric Mucosa Ulceration Simulation显示文摘 | Yakubtsova Iryna Volodymyrivna Khilko Tetyana Dmytrivna Voronin Evgeniy Pylypovych Preobrazhenska Tamara Dmytrivna Ostapchenko Lyudmyla Ivanivna Makai Sandor | 2014 | 材料科学与工程(中英文A版)2014，4，9： | 0 |
| 16 | Methodological approach to the isolation of functionally active proteins from the tissues of marine hydrobionts: an example of Adamussium colbecki显示文摘Enzymes from cold-adapted organisms have significant application potential. Because of their unique properties they have been found to be useful in various industries. Despite indisputable practical interest, cold active enzymes also represent a valuable model for fundamental research into protein folding and catalysis. Many investigators have focused their attention on marine hydrobionts, which are growing in importance as a promising source of enzymes. The nature of the source not only determines the availability and the cost of biomolecules of interest but also determines the choice of method for their extraction. A simple and convenient methodological approach of two-stage extraction of proteins has been tested on the Antarctic marine hydrobiont—Adamussium colbecki. This method extracts enough effective protein directly from primary raw materials, as well as when using leftover crude precipitates. The electrophoretic pattern of proteins showed the presence of molecules in a wide range of molecular weights in the samples of A. colbecki after the first and the second stage of extraction. The general proteolytic activity in the first and the second extracts were examined using a zymogram technique. Our experiments revealed that the second extract of A. colbecki contained thermo stable protease exhibiting a molecular weight of 95 k Da in a gelatin zymogram. Further biochemical assays, using different substrates, were conducted to partially identify the types of hydrolases present in the first and the second extracts. Our results revealed the presence of enzymes with collagenolytic and some amylolytic activities preserved in the second extracts. But no esterase or amidase trypsin-like activities were found in the second extract, in contrast to the first extract where this type of activity was significant. | Nataliia Raksha Dmytro Gladun Oleksii Savchuk Lydmila Ostapchenko | 2015 | Advances in Polar Science2015，26，4： | 0 |
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