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| 1 | Therapeutic effect of Qingyi decoction in severe acute pancreatitis-induced intestinal barrier injury显示文摘AIM: To investigate the effect of Qingyi decoction onthe expression of secreted phospholipase A2(s PLA2) in intestinal barrier injury.METHODS: Fifty healthy Sprague-Dawley rats were randomly divided into control, severe acute pancreatitis(SAP), Qingyi decoction-treated(QYT), dexamethasonetreated(DEX), and verapamil-treated(VER) groups. The SAP model was induced by retrograde infusion of 1.5% sodium deoxycholate into the biliopancreatic duct of the rats. All rats were sacrificed 24 h post-SAP induction. Arterial blood, intestine, and pancreas from each rat were harvested for investigations. The levels of serum amylase(AMY) and diamine oxidase(DAO) were determined using biochemical methods, and serum tumor necrosis factor(TNF)-α level was measured by an enzyme linked immunosorbent assay. Pathologic changes in the harvested tissues were investigated by microscopic examination of hematoxylin and eosinstained tissue sections. The expressions of s PLA2 at m RNA and protein levels were detected by reverse transcriptase PCR and Western blot, respectively. A terminal deoxynucleotidyl transferase-mediated d UTP nick-end labeling assay was used to investigate apoptosis of epithelial cells in the intestinal tissues. RESULTS: Compared to the control group, the expression of s PLA2 at both the m RNA and protein levels increased significantly in the SAP group(0.36 ± 0.13 vs 0.90 ± 0.38, and 0.16 ± 0.05 vs 0.64 ± 0.05, respectively; P s < 0.01). The levels of AMY, TNF-α and DAO in serum were also significantly increased(917 ± 62 U/L vs 6870 ± 810 U/L, 59.7 ± 14.3 ng/L vs 180.5 ± 20.1 ng/L, and 10.37 ± 2.44 U/L vs 37.89 ± 5.86 U/L, respectively; P s < 0.01). The apoptosis index of intestinal epithelial cells also differed significantly between the SAP and control rats(0.05 ± 0.02 vs 0.26 ± 0.06; P < 0.01). The serum levels of DAO and TNF-α, and the intestinal apoptosis index significantly correlated with s PLA2 expression in the intestine(r = 0.895, 0.893 and 0.926, respectively; Ps < 0.05). Thelevels of s PLA2, AMY, TNF-α, and DAO in the QYT, VER, and DEX groups were all decreased compared with the SAP group, but not the control group. Qingyi decoction intervention, however, gave the most therapeutic effect against intestinal barrier damage, although the onset of its therapeutic effect was slower. CONCLUSION: Qingyi decoction ameliorates acute pancreatitis-induced intestinal barrier injury by inhibiting the overexpression of intestinal s PLA2. This mechanism may be similar to that of verapamil. | Jing-Wen Zhang Gui-Xin Zhang Hai-Long Chen Ge-Liang Liu Lawrence Owusu Yu-Xi Wang Guan-Yu Wang Cai-Ming Xu | 2015 | World Journal of Gastroenterology2015，21，12： | 35 |
| 2 | Gut microbial diversity analysis using Illumina sequencing for functional dyspepsia with liver depression-spleen deficiency syndrome and the interventional Xiaoyaosan in a rat model显示文摘AIM To investigate gut microbial diversity and the interventional effect of Xiaoyaosan(XYS) in a rat model of functional dyspepsia(FD) with liver depression-spleen deficiency syndrome. METHODS The FD with liver depression-spleen deficiency syndrome rat model was established through classic chronic mild unpredictable stimulation every day. XYS group rats received XYS 1 h before the stimulation. The models were assessed by parameters including state ofthe rat, weight, sucrose test result and open-field test result. After 3 wk, the stools of rats were collected and genomic DNA was extracted. PCR products of the V4 region of 16 S rD NA were sequenced using a barcoded Illumina paired-end sequencing technique. The primary composition of the microbiome in the stool samples was determined and analyzed by cluster analysis.RESULTS Rat models were successfully established, per data from rat state, weight and open-field test. The microbiomes contained 20 phyla from all samples. Firmicutes, Bacteroidetes, Proteobacteria, Cyanobacteria and Tenericutes were the most abundant taxonomic groups. The relative abundance of Firmicutes, Proteobacteria and Cyanobacteria in the model group was higher than that in the normal group. On the contrary, the relative abundance of Bacteroidetes in the model group was lower than that in the normal group. Upon XYS treatment, the relative abundance of all dysregulated phyla was restored to levels similar to those observed in the normal group. Abundance clustering heat map of phyla corroborated the taxonomic distribution. CONCLUSION The microbiome relative abundance of FD rats with liver depression-spleen deficiency syndrome was significantly different from the normal cohort. XYS intervention may effectively adjust the gut dysbacteriosis in FD. | Juan-Juan Qiu Zhe Liu Peng Zhao Xue-Jun Wang Yu-Chun Li Hua Sui Lawrence Owusu Hui-Shu Guo Zheng-Xu Cai | 2017 | World Journal of Gastroenterology2017，23，5： | 23 |
| 3 | CNP signal pathway up-regulated in rectum of depressed rats and the interventional effect of Xiaoyaosan显示文摘AIM:To investigate the distribution and expressionof C-type natriuretic peptide(CNP)/natriuretic peptide receptor B(NPR-B) in the rectum of a rodent depression model and the interventional effect of Xiaoyaosan(XYS).METHODS:Male rats(n = 45) of clean grade(200 ± 20 g) were divided into five groups after one week of adaptive feeding:primary control,depression model,low dose XYS,middle dose XYS,and high dose XYS.The animal experiment continued for 3 wk.Primary controls were fed normally ad libitum.The rats of all other groups were raised in solitary and exposed to classic chronic mild unpredictable stimulation each day.XYS groups were perfused intragastrically with low dose,middle dose,and high dose XYS one hour before stimulation.Primary control and depression model groups were perfused intragastrically with normal saline under similar conditions as the XYS groups.Three weeks later,all rats were sacrificed,and the expression levels of CNP and NPR-B in rectum tissues were analyzed by immunohistochemistry,real-time polymerase chain reaction,and Western blotting.RESULTS:CNP and NPR-B were both expressed in the rectum tissues of all rats.However,the expression levels of CNP and NPR-B at both gene and protein levels in the depression model group were significantly higher when compared to the primary control group(n = 9; P < 0.01).XYS intervention markedly inhibited the expression levels of CNP and NPR-B in depressed rats.The expression levels of CNP and NPR-B in the high dose XYS group did not significantly differ from the expression levels in the primary control group.Additionally,the high and middle dose XYS groups(but not the low dose group) significantly exhibited lower CNP and NPR-B expression levels in the rectum tissues of the respectively treated rats compared to the untreated depression model cohort(n = 9; P < 0.01).CONCLUSION:The CNP/NPR-B pathway is upregulated in the rectum of depressed rats and may be one mechanism for depression-associated digestive disorders.XYS antagonizes this pathway at least partially. | Ping Li Xu-Dong Tang Zheng-Xu Cai Juan-Juan Qiu Xue-Lian Lin Tong Zhu Lawrence Owusu Hui-Shu Guo | 2015 | World Journal of Gastroenterology2015，21，5： | 8 |
| 4 | 关于昆虫DNA甲基化的研究进展(英文)显示文摘DNA甲基化作为重要的表观遗传调控现象之一,广泛地存在于原核生物和真核生物中,并且在多种生物学过程中起到非常重要的作用。近年来,随着DNA甲基化研究方法的改进及创新,昆虫DNA甲基化的研究取得了一些新的进展和成果。本文就果蝇和家蚕等昆虫DNA甲基化的特征、研究方法,以及DNA甲基化在昆虫基因组印迹、胚胎发育、衰老、记忆、免疫和进化中的作用等进行简要综述,以期对家蚕等昆虫DNA甲基化的本质及功能理解有所启发。 | Annan Enoch 姜晓旭 商琪 Dweteh Owusu Amanfo 吴萍 郭锡杰 | 2018 | 蚕业科学2018，44，3： | 3 |
| 5 | Impact of immunosuppression minimization and withdrawal in long-term hepatitis C virus liver transplant recipients显示文摘AIM:To investigate the effects of different immunosuppressive regimens and avoidance on fibrosis progression in hepatitis C virus(HCV)liver transplant(LT)recipients.METHODS:We retrospectively compared the liver biopsies of well-matched HCV LT recipients under calcineurin inhibitors(CNI group,n=21)and mycophenolate(MMF group,n=15)monotherapy,with those patients who successfully withdrawn immunosuppression(IS)therapy from at least 3 years(TOL group,n=10).To perform the well-matched analysis,all HCV transplanted patients from December 1993 were screened.Only those HCV patients who reached the following criteria were considered for the analysis:(1)at least3 years of post-operative follow-up;(2)patients with normal liver graft function under low dose CNI monotherapy(CNI group);(3)patients with normal liver graft function under antimetabolite(Micophenolate Mofetil or coated mycophenolate sodium)monotherapy(MMF group);and(4)recipients with normal liver function without any IS.We excluded from the analysis recipients who were IS free or under monotherapy for<36 mo,recipients with cirrhosis or with unstable liver function tests.RESULTS:Thirty six recipients were enrolled in the study.Demographics,clinical data,time after LT and baseline liver biopsies were comparable in the three groups.After six years of follow-up,there was no worsening of hepatic fibrosis in the MMF group(2.5±1.5Ishak Units vs 2.9±1.7 Ishak Units,P=0.5)and TOL group(2.7±10.7 vs 2.5±1.2,P=0.2).In contrast,a significant increase in the fibrosis score was observed in the CNI group(2.2±1.7 vs 3.9±1.6,P=0.008).The yearly fibrosis progression rate was significantly worse in the CNI group(0.32±0.35)vs MMF group(0.03±0.31,P=0.03),and TOL group(-0.02±0.27,P=0.02).No differences have been reported in grading scores for CNI group(2.79±1.9,P=0.7),MMF group(3.2±1.5,P=0.9)and TOL group(3.1±1.4,P=0.2).Twenty four patients were treated with low dose ribavirin(8TOL,7 MMF,9 CNI).The hepatitis C titers were comparable in the three groups.No episodes of rejection have been reported despite differences of liver function test in the three groups during the observational period.CONCLUSION:IS withdrawal and MMF monotherapy is safe and seems to be associated with the slowest fibrosis progression in HCV LT recipients. | Tommaso Maria Manzia Roberta Angelico Paolo Ciano Jon Mugweru Kofi Owusu Daniele Sforza Luca Toti Giuseppe Tisone | 2014 | World Journal of Gastroenterology2014，20，34： | 3 |
| 6 | MoS2/MnO2 heterostructured nanodevices for electro- chemical energy storage显示文摘 | Xiaobin Liao Yunlong Zhao Junhui Wang Wei Yang Lin Xu Xiaocong Tian Yi Shuang Kwadwo Asare Owusu Mengyu Yan Liqiang Mai | 2018 | Nano Research2018，11，4： | 2 |
| 7 | Iron binding characteristics of phenolic compounds: some tentative structure–activity relations显示文摘 | Santosh Khokhar Richard.K. Owusu Apenten | 2002 | Food Chemistry2002，，1： | 2 |
| 8 | 从Zn—Cd—Co—Ni的硫酸盐溶液中氧化淀淀Co显示文摘研究了利用Caro酸将Co从Zn-Cd-Co-Ni的硫酸盐溶液中沉淀出来的工艺。Caro酸把Co(Ⅱ)氧化成Co(Ⅲ)。在PH3.5-4.0,料液中大约98-99%的钴以Co(OH)3形式沉淀下来,但PH≤2.5时,料液中只有不到8%的钴被沉淀,并产生难于过滤的墨绿色胶状悬浮物。由间歇性实验和连续性实验得到的结果相近。的Co(OH)3沉淀物的含钴量为47%-49%,非常接近53.6%的理论值。 | Owusu,G 何洪涛 | 1999 | 株冶科技1999，27，2： | 2 |
| 9 | Macroscopic synthesis of ultrafine N–doped carbon nanofibers for superior capacitive energy storage显示文摘Carbon nanofibers(CNFs)with excellent electric conductivity and high surface area have attracted immense research interests in supercapacitors.However,the macroscopic production of CNFs still remains a great challenge.Herein,ultrafine N–doped CNFs(N–CNFs)with a diameter of$20 nm are synthesized through a simple and scalable sol-gel method based on the self-assembly of phenolic resin and cetyltrimethylammonium bromide.When employed in aqueous supercapacitors,the obtained activated N–CNFs manifest a high gravimetric/areal capacitance(380 Fg-1/1.7 Fcm-2)as well as outstanding rate capability and cycling stability.Besides,the activated N–CNFs also demonstrate excellent capacitive performance(330 Fg-1)in flexible quasi-solid-state supercapacitors.The remarkable electrochemical performance as well as the easy and scalable synthesis makes the N–CNFs a highly promising electrode material for supercapacitors. | Qiang Yu Jianshuai Lv Zhenhui Liu Ming Xu Wei Yang Kwadwo Asare Owusu Liqiang Mai Dongyuan Zhao Liang Zhou | 2019 | Science Bulletin2019，64，21： | 2 |
| 10 | A shortchannel DC SPICE model for polysilicon thin-film transistors including temperature effects 显示文摘 | Jacunski M D Shur M S Owusu A A | 1999 | IEEE Transactions on Electron Devices1999，46，6： | 1 |
| 11 | Stabilization and Partial Purification of a Protcase from Ginger Rhizome显示文摘 | Adulyatham Richard Owusu - Apenten | 2005 | J Food Scie2005，70，31： | 1 |
| 12 | Chemical and physicalchanges in red hake fillets during frozen storagel显示文摘 | Owusu Ansah Y J Hultin H O | 1986 | Journal of Food Science1986，，51： | 1 |
| 13 | Preparation of fine protein-stabilized water-in-oil-water emulsions显示文摘 | Dickinson E Evison J Owusu R K | 1991 | Food Hydrocolloids1991，5，5： | 1 |
| 14 | A Short-Channel DC SPICE Model for Polysilicon Thin-Film Transistors Including Temperature Effects显示文摘 | Jacunski M D Shur M S Owusu A A Ytterdal T Hack M Iniguez B | 1999 | Electron Devices Transactions on1999，46，6： | 1 |
| 15 | Selective extraction of copper from acidic zinc sulfate leach solution using LIX622 显示文摘 | Owusu G | 1999 | Hydrometallurgy1999，51，： | 1 |
| 16 | Future of NSP- degrading enzymes to improve nutrient utilization of co- products and gut health in pigs 显示文摘 | Zijlstra R T Owusu - Asiedu A Simmins P H | 2010 | Livestock Science2010，134，13： | 1 |
| 17 | Effect of surfac- rants on zinc and iron dissolution rates during oxidative leaching of sphalerite 显示文摘 | Owusu G Dreisinger D B Peters E | 1995 | Hydrometallurgy1995，38，3： | 1 |
| 18 | Spray drying an appropriate technology for food and pharmaceutical industry显示文摘 | AFOAKWAH A N ADOMAKO C OWUSU J | 2012 | J Environ Sci Comp Sci and Eng Technol2012，1，： | 1 |
| 19 | Analysis of the curing behavior of cyanate ester resin systems 显示文摘 | OSEI - OWUSU A MARTIN G C GOTRO J T | 1991 | Polym Eng & Sci1991，，31： | 1 |
| 20 | Treatment of Intracranial Rat Glioma Model with Implant of Radiosensitizer and Biomodulator Drug Combined with Extenal Beam Radiotheyapy显示文摘 | Li Y Owusu A Lehnert S | 2004 | Int J Radiat Oncol Biol Phvs2004，58，2： | 1 |
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