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| 1 | CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C virus-coinfected patients显示文摘AIM To characterize peripheral blood natural killer(NK) cells phenotypes by flow cytometry as potential biomarker of liver fibrosis in human immunodeficiency virus(HIV)/hepatitis C virus(HCV) coinfected patients.METHODS Peripheral mononuclear cells from 24 HIV/HCV(HBVnegative) coinfected and 5 HIV/HCV/HBV seronegative individuals were evaluated. HIV/HCV coinfected patients were divided in to groups: G1, patients with METAVIR F0-F2 and G2, patients with METAVIR F3-F4. NK surface cell staining was performed with: AntiCD3(APC/Cy7), anti-CD56(PE/Cy5), anti-CD57(APC), anti-CD25(PE), anti-CD69(FITC), anti-NKp30(PE), antiNKp46(PE/Cy7), anti-NKG2D(APC), anti-DNAM(FITC); anti-CD62L(PE/Cy7), anti-CCR7(PE), anti-TRAIL(PE), anti-Fas L(PE), anti CD94(FITC). Flow cytometry data acquisition was performed on BD FACSCanto, analyzed using Flow Jo software. Frequency of fluorescence was analyzed for all single markers. Clinical records were reviewed, and epidemiological and clinical data were obtained.RESULTS Samples from 11 patients were included in G1 and from 13 in G2. All patients were on ARV, with undetectable HIV viral load. Liver fibrosis was evaluated by transient elastography in 90% of the patients and with biopsy in 10% of the patients. Mean HCV viral load was(6.18 ± 0.7 log10). Even though, no major significant differences were observed between G1 and G2 regarding NK surface markers, it was found that patients with higher liver fibrosis presented statistically lower percentage of NK cells than individual with low to mild fibrosis and healthy controls(G2: 5.4% ± 2.3%, G1: 12.6% ± 8.2%, P = 0.002 and healthy controls 12.2% ± 2.7%, P = 0.008). It was also found that individuals with higher liver fibrosis presented lower CD4 LT count than those from G1(G2: 521 ± 312 cells/μL, G1: 770 ± 205 cells/μL; P = 0.035).CONCLUSION Higher levels of liver fibrosis were associated with lower percentage of NK cells and LTCD4+ count; and they may serve as noninvasive biomarkers of liver damage. | Natalia Laufer Diego Ojeda María Laura Polo Ana Martinez Héctor Pérez Gabriela Turk Pedro Cahn Norberto Walter Zwirner Jorge Quarleri | 2017 | World Journal of Hepatology2017，9，25： | 2 |
| 2 | Effect of pharmacologically induced mydriasis on the normal variability of retinal oscillatory potentials in man 显示文摘 | Polo A di Summa A Perlini S | 1995 | Acta Ophthalmol Scand1995，73，2： | 2 |
| 3 | The perceived value of the rural tourism stay and its effect on rural tourist behaviour 显示文摘 | Ana Isabel Polo Pen-a Dolores Mar'la Fr'las Jamilena and Miguel A' ngel Ikodr'lguez Molina | 2012 | Journal of Sustainable Tourism2012，20，8： | 1 |
| 4 | Timing of repeat elective eaesare- andelivery and neonatal respiratory outcomes显示文摘 | Farchi S Di Lallo D Polo A | 2010 | Arch Dis Child Fetal Neonatal Ed2010，95，1： | 1 |
| 5 | Learning in chemistry with virtual laboratories显示文摘 | Jimenez P M Pedrajas A P Polo J | 2003 | Journal of Chemieal Edueation2003，80，3： | 1 |
| 6 | An in vivo model of human proliferative scar 显示文摘 | Polo M Kim YJ Kucukcelebi A | 1998 | J Surg Res1998，74，2： | 1 |
| 7 | MR and CT image fusion for postimplant analysis in permanent prostate seed implants显示文摘 | Polo A Cattani F Vavassori A | 2004 | Int J Radiat Oncol Biol Phys2004，60，： | 1 |
| 8 | Spinal and bulbar muscular atrophy:skeletal muscle pathology in male patients and heterozygous females显示文摘 | Sorarù G D'Ascenzo C Polo A | 2008 | J Neurol Sci2008，264，： | 1 |
| 9 | 显示文摘 | SANCHEZ - POLO M RIVERA - UTRILLA J ZAROR C A | 2002 | J Chem Technol Biotechnol2002，77，： | 1 |
| 10 | Fibroblast growth factor-2 gene delivery stimulates axon growth by adult retinal ganglion cells after acute optic nerve injury显示文摘 | Sapieha PS Peltier M Rendahl KG Manning WC Di Polo A | 2003 | Mol Cell Neurosci2003，24，3： | 1 |
| 11 | Enhanced survival and regeneration of axotomized retinal neurons by repeated delivery of cell-permeable C3-1ike Rho antagonists 显示文摘 | Bertrand J Polo A D McKerracher L | 2007 | Neurobiol Dis2007，25，1： | 1 |
| 12 | MR and CT image fu-sion for post implant analysis in permanent prostate seed implants显示文摘 | Polo A Cattani F Vavassori A | 2004 | International Journal of Radiation Oncology Biol-ogy Physics2004，60，5： | 1 |
| 13 | 显示文摘 | DURAN J OLIVER D POLO A | 2003 | Tatrahedron: Asymmetry2003，14，17： | 1 |
| 14 | Layered amplification of gene expression with a DNA gene delivery system显示文摘 | Latham E M Polo J M | 1995 | Ann N Y Acad Sci1995，27，772： | 1 |
| 15 | Formation of biogenic amines throughout the in- dustrial manufacture of red wine 显示文摘 | MARCOBAL A MARTIN-ALVAREZ P J POLO M C | 2006 | Journal of Food Protection2006，69，2： | 1 |
| 16 | Compressive wide-band spectrum sensing显示文摘 | Polo YL Wang Y Pandharipande A | 2009 | IEEE Int Corif Acoustics Speech and Signal Processing2009，30，7： | 1 |
| 17 | Generating Three-tier Applications from Relational Databases显示文摘 | Polo M Gomez J A Piattini M | 2002 | A Formal and Practical Approach Information and Software Technology2002，44，15： | 1 |
| 18 | Enhanced survival and regeneration of axotomized retinal neurons by d repeated elivery of cell-permeable C3-like Rho antagonists显示文摘 | Bertrand J Di Polo A McKerracher L | 2007 | Neurobiol Dis2007，25，1： | 1 |
| 19 | An in vivo model of human proliferative scar显示文摘 | Polo M Kim YJ Kucukcelebi A | 1998 | J Surg Res1998，74，： | 1 |
| 20 | Peripheral and segmental spinal abnormalities of median and ulnar somatosensory evoked potentials in Hirayama' s disease 显示文摘 | Polo A Curro'Dossi M Fiaschi A | 2003 | J Neurol Neurosurg Psychiatry2003，74，5： | 1 |
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