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| 1 | 富含DHA的鸡蛋黄和L-半胱氨酸盐酸盐添加物对冷冻猪精液质量的影响显示文摘本文旨在研究富含DHA的鸡蛋黄和L-半胱氨酸添加物对冷冻猪精液质量的影响。从5只皮特兰猪种获得15个射精样本,根据冷冻添加物的组成将其分为4组:正常鸡蛋黄组(组1),富含DHA的鸡蛋黄组(组2),添加了5mmol/L半胱氨酸添加物的正常鸡蛋黄(组3),添加了5mmol/L的半胱氨酸添加物的富含DHA的鸡蛋黄(组4)。精液冷冻保存过后在50℃下解冻12秒。之后检测精子直线前进运动,精子生存力,顶体完整性,解冻精液中精子质膜的功能完整性。只添加IL-半胱氨酸(组3)可以提高精子直线前进运动(P〈0.05),与富含DHA的鸡蛋黄一起添加(组4)既能增加直线前进运动(P〈0.05)又能改善顶体完整性(P〈0.01)。富含DHA的鸡蛋黄单独添加对解冻后精液的质量没有任何作用(P〉0.05)。总之,抗氧化的三.半胱氨酸单独或者与富含DHA的鸡蛋黄一起作用可以明显改善解冻后精液质量,尤其是直线前进运动力和顶体完整性。 | Panida Chanapiwat Kampon Kaeoket Padet Tummaruk | 2009 | Asian Journal of Andrology2009，11，5： | 5 |
| 2 | Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen显示文摘Cryopreservation 与反应的氧种类的生产被联系,它导致精子膜并且因而的类脂化合物 peroxidation 在精子活动性和减少的富饶潜力的减小。这研究的目的是决定为雄野猪精液的 cryopreservation 需要的 L 半胱氨酸的最佳的集中。12 只雄野猪为这研究提供了证明活动性和形态学的精液。精液被划分成乳糖鸡蛋蛋黄( LEY )扩展程序在使用了到的四部分重新推迟 centrifuged 精子小团与L半胱氨酸的各种各样的集中被补充到达 0 mmol L ? 1 (组我,控制), 5 mmol L ? 1 (组 II ), 10 mmol L ? 1 (组 III )并且 15 mmol L ? 1 (组 IV )。精液暂停在稻草(0.5 mL ) 被装载并且在一个控制率冰箱放了。在 cryopreservation 以后,冻结精液样品为进步活动性,生存能力使用 SYBR-14/EthD-1 用染色的 FITC-PNA/EthD-1 染色和 acrosome 正直被融化并且调查。有一显著地更高(P < 0.01 ) 在与控制相比的二个 L-cysteine-supplemented 组(组 II 和组 III ) 的进步活动性,生存能力和 acrosomal 正直的百分比。有 L 半胱氨酸的 biphasic 效果,与在组 III 的进步活动性,生存能力和 acrosomal 正直的最高的百分比。在结论, 5 或 10 mmol L ? 1 是为改进冻结的鈥搕h awed 雄野猪精液的质量要加到 LEY 扩展程序的 L 半胱氨酸的最佳集中。 | Kampon Kaeoket Panida Chanapiwat Padet Tummaruk Mongkol Techakumphu | 2010 | Asian Journal of Andrology2010，12，5： | 4 |
| 3 | Prevalence of ocular demodicosis among patients at Tertiary Care Center,Bangkok,Thailand显示文摘● AIM: To determine the prevalence of ocular demodicosis by both microscopic examination and molecular detection among patients at King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok.● METHODS: One hundred individuals were enrolled in the study and were divided into five age groups. The meibomian gland dysfunction(MGD) score and qualities of cylindrical dandruff(CD) were also determined. Demodex mite infestations of eyelash samples were screened by both microscopic examination and semi-nested polymerase chain reaction(PCR).● RESULTS: The prevalence of ocular demodicosis as determined by microscopic examination was 42% [Demodex folliculorum(D. folliculorum) 41% and Demodex brevis(D. brevis) 1%]. Among patients who had ocular Demodex infestation, 69% have CD and had an average MGD score of 4; in patients without demodicosis, 15.5% had CD and had an average MGD score of 4.12. Prevalence of ocular demodicosis as determined by semi-nested PCR was 79%(D. folliculorum 78% and D. brevis 1%). ● CONCLUSION: This is the first report on the prevalence of ocular demodicosis in Thailand. Patients with CD also had Demodex mites present. Semi-nested PCR is better than microscopy for Demodex infestation detection. An extensive survey with more representative samples is required to determine the prevalence in the country. | Ngamjit Kasetsuwan Kitchaporn Kositphipat Mathu Busayarat Pawanrat Threekhan Kanok Preativatanyou Atchara Phumee Padet Siriyasatien | 2017 | International Journal of Ophthalmology(English edition)2017，10，1： | 3 |
| 4 | Impact of porcine epidemic diarrhea virus infection at different periods of pregnancy on subsequent reproductive performance in gilts and sows显示文摘 | Olanratmanee Annop Kunavongkrit Padet Tummaruk | 2010 | Animal Reproduction Science2010，122，12： | 1 |
| 5 | Numerical Study of Heat Transfer and Fluid Flow in a Power Transformer显示文摘 | Wakil N E Chereches N C Padet J | 2006 | International Journal of Thermal Sciences2006，45，6： | 1 |
| 6 | The time constant of double pipe and one pass shell-and-tube heat exchangers in the case of varying fluid flow rates显示文摘 | Lachi M Wakil N El Padet J | | International Journal of Heat and Mass Transfer0，1997，： | 1 |
| 7 | Numerical Study of Heat Transfer and Fluid Flow in a Power Transformer显示文摘 | Wakil N E Chereches N C Padet J | 2006 | International Jgurnal of Thermal Sciences2006，45，6： | 1 |
| 8 | nformation semantics of possibilistic uncertainty显示文摘 | Ramer A Padet C | 1995 | Fuzzy Sets and Systems1995，69，： | 1 |
| 9 | Numerical study of heat transfer and fluid flow in a power transformer显示文摘 | E1 Wakil N Chereches N-C Padet J | 2006 | International Journal of Thermal Sciences2006，45，6： | 1 |
| 10 | Laminar boundary-layer flow over a semi-infinite plate impulsively heated or cooled显示文摘 | Rebay M Padet J | 1999 | Eur Phys J AP1999，7，3： | 1 |
| 11 | Impact of porcine epidemic diarrhea virus infection at different periods of pregnancy on subsequent reproductive performance in gilts and sows显示文摘 | Em-on Olanratmanee Annop Kunavongkrit Padet Tummaruk | 2010 | Animal Reproduction Science2010，，1： | 1 |
| 12 | Transient laminar forced convection from a wedge flow显示文摘 | Rebay M Padet J | 2004 | Internat Comm Heat Mass Transfer2004，31，4： | 1 |
| 13 | Transient convective heat transfer显示文摘 | Padet J | 2005 | J Braz Soc Mech Sci & Eng2005，27，1： | 1 |
| 14 | Dose-dependent inhibi tion of BrdU detection in the cell proliferation ELISA by cul ture medium proteins 显示文摘 | Padet L St-Amour I Aubin E | 2009 | J Immunoassay Immunochem2009，30，3： | 1 |
| 15 | Numerical study of heat transfer and fluid flow in a power transformer 显示文摘 | Wakil N El Chereches N C Padet J | 2006 | In- ternational Journal of Thermal Sciences2006，45，6： | 1 |
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