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| 1 | Immunocytochemical identification and localization of APUD cells in the gut of seven stomachless teleost fishes显示文摘AIM To study the cell types,localization,distribution density and morphology of APUDcells in the intestinal mucosa of stomachlessteleost fishes.METHOD By using the peroxidase-antiperoxidase complex(PAP)immunocytochemical staining technique theidentification,localization and morphology ofimmunoreactive(IR)endocrine cells seattered inthe intestinal mucosa of grass carp(Cyenopharyngodon idellus),black carp(Mylopharyngodon piceus)and common carp(Cyprinus carpio)were investigated with 20kinds of antisera prepared against mammalianpeptide hormones of APUD cells,and likewise byusing avidin-biotin-peroxidase complex(ABC)method those of silver carp(Hypophthalmichthys molitrix),bighead(Aristichthys nobilis),silver crucian carp(Carassius gibelio)and bluntnose black bream(Megalobrama amblyocephala)were alsostudied with 5 different antisera.Thereplacement of the first antiserum by phosphatebuffered saline(PBS)was employed as a control.IR endocrine cells were counted with asquare-mesh ocular micrometer from 10 fieldsselected randomly in every section of each partof the intestine specimen.The average numberof IR endocrine cells per mm2 was counted toquantify their distribution density.RESULT Gastrin(GAS)-,Gastric inhibitorypeptide(GIP)-,glucagon(GLU)-,glucagon-likeimmunoreactants(GLI)-,bovine pancreaticpolypeptide(BPP)-,leucine-enkephalin(ENK)-and substance P(SP)-IR endocrine cells werefound in the gut of grass carp,black carp andcommon carp,and somatostatin(SOM)-IRendocrine cells were only seen in common carp.GAS-,GIP-and GLU-IR endocrine cells werefound in the intestinal mucosa of silver carp,bighead,silver crucian carp and bluntnose blackbream.Most of IR endocrine cells had the higherdistribution density in the foregut and midgut,and were longer in shape.They had a long apicalcytoplasmic process extended to the gut lumenand a basal process extended to adjacent cellsor basement membrane and touched with it.Sometimes,the basal cytoplasmic processformed an enlarged synapse-like structure in thecontiguous part with basement membrane.Thisphenomenon provided new morphologicalevidence for neuroendocrine and paracrinesecretory function of these enteroendocrinecells.CONCLUTION At least 8 kinds of IR endocrinecells were found in the gut of stomachlessteleost species for the first time in China.TheseIR endocrine cells scattering in the gut mucosabelong to the APUD system.Among them,thehormones secreted by SP-,ENK-,SOM-and GLU-IR endocrine cells belong to the peptides of dualdistribution in the brain and gut.This providednew evidence for the concept of brain-gutpeptide.According to the cell types,distribution density,morphologicalcharacteristics and variety in shape of APUDcells in the gut of stomachless teleost fishes,itis deemed that the digestive tract of fishes isalso an endocrine organ of great importance andcomplexity. | Pan QS Fang ZP Zhao YX | 2000 | World Journal of Gastroenterology2000，6，1： | 25 |
| 2 | Mineralogical alteration of thermally treated siderite in air: Mossbauer spectroscopy results显示文摘Mineralogical alternation of thermally treated siderite in air atmosphere has been systematically analyzed by Mossbauer effects. It was preliminarily estimated from the area of sub-spectra that 4% , 39% and 62% of magnetite were formed at 410℃, 490℃ and 510℃ respectively. After being incrementally heated at 530℃ the spectra consist of two sextets of Fe3O4. Sextet of γ-Fe2O3 with hyperfine field of 50T was observed at 550℃. Spectra at 580℃ | Pan, YX Zhu, RX Ping, JY | 1999 | Chinese Science Bulletin1999，44，18： | 2 |
| 3 | Antisense mapping of MOR-1 in rats: distinguishing between morphine and morphine-6 beta-glucuronide antinociception显示文摘 | Leventhal L Pan YX | 1997 | J Pharmacol Exp Ther1997，281，1： | 1 |
| 4 | Effects of intermedin(1-53)on cardiac function and ischemia/reperfusion injury in isolated rat hearts显示文摘 | Yang JH Jia YX Pan CS | 2005 | Biochem Biophys Res Commun2005，327，： | 1 |
| 5 | Differential effects of cardiac sympathetic afferent stimulation on neurons in the nucleus tractus solitarius显示文摘 | Wang WZ Gao L Pan YX | 2006 | Neurosci Lett2006，409，2： | 1 |
| 6 | Intermedin1-53 activates L-arginine/nitric oxide synthase/nitric oxide pathway in rat aortas显示文摘 | Yang JH Pan CS Jia YX | 2006 | Biochem Biophys Res Commun2006，341，2： | 1 |
| 7 | ATI receptors in the nucleus tractus solitarii mediate the interaction between the baroreflex and the cardiac sympathetic afferent reflex in anesthetized rats显示文摘 | Wang WZ Gao L Pan YX | 2007 | Am J Physiol2007，292，： | 1 |
| 8 | Intermedin ( I - 53 ) protects the heart against isoproterenol -induced ischemic injury in rats 显示文摘 | Jia YX Yang J H Pan C S | 2006 | Eur J Pharmaco12006，549，13： | 1 |
| 9 | Blockade of U50,488Hanalgesia by antisense oligodeoxynucleotides to a kappa-opioid receptor显示文摘 | Brown G Pan YX | 1994 | Eur J Pharmacol1994，253，3： | 1 |
| 10 | Activat ion of nuclear factor-κB in the brain after experimental subarachnoid hemorrhage and its potential role in delayed brain injury显示文摘 | You WC Wang CX Pan YX | | 0，，3： | 1 |
| 11 | Cloning and functional characterization through antisense mapping of a kappa 3-related opioid receptor显示文摘 | Cheng J Xu J | 1995 | Mol Pharmacol1995，47，6： | 1 |
| 12 | Delayed cytoprotection induced by hypoxic preconditioning in cultured neonatal rat cardiomyocytes:role of GRP78显示文摘 | Pan YX Ren AJ Zheng J | 2007 | Life Sci2007，81，13： | 1 |
| 13 | Newphenanthrene glycosides from Dendrobium denneanum and theircytotoxic activity 显示文摘 | Li F Pan HM Liu X Chen B Tang YX Xi XJ Wang MK | 2013 | Phytochem Lett2013，6，： | 1 |
| 14 | Hypoxia can contribute to the induction of the Epstein-Barr virus (EBV) lytic cycle 显示文摘 | Jiang JH Wang N Li A Liao WT Pan ZG Mai SJ Li DJ Zeng MS Wen JM Zeng YX | 2006 | J Clin Virol2006，37，2： | 1 |
| 15 | Expression of FasL and its interaction with Fas are mediated by c-Jun N-terminal kinase(JNK)pathway in 6-OHDA-induced rat model of parkinson disease显示文摘 | Pan J Zhao YX Wang ZQ | | 0，，2： | 1 |
| 16 | Distribution of sorbed phenanthreneand pyrene in different humic fractions of soils andimportance of humin显示文摘 | Pan B Xing BS Liu WX Tao S Lin XM Zhang YX XiaoY Dai HC Yuan HS | 2006 | Environmental Pollution2006，143，3： | 1 |
| 17 | Antioxidant activity of phosphorylated exopolysaccharide produced by Lactococcus lactis subsp, lactis显示文摘 | Guo YX Pan DD Sun YY | 2013 | Carbohydrate Polymers2013，97，2： | 1 |
| 18 | Expression of FasL and its interaction with Fas are mediated by c-Jun N terminal kinase (JNK) pathway in 6-OHDA-indueed rat model of Parkinson disease 显示文摘 | Pan J Zhao YX Wang ZQ | 2007 | Neurosci Lett2007，428，23： | 1 |
| 19 | Multiresidue method for thedetermination of pesticides in fruits and vegetables using gaschromatography - negative chemical ionization - triple quadrupole tandemmass spectrometry显示文摘 | Dong J Pan YX Lv JX | 2007 | Chromatographia2007，74，： | 1 |
| 20 | Relationship between immune parameters and organ involvement in children with henoch- schonlein purpura显示文摘 | Pan YX Ye Q Shao WX | 2014 | PLoS One2014，9，11： | 1 |
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