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| 1 | Golgi protein-73:A biomarker for assessing cirrhosis and prognosis of liver disease patients显示文摘BACKGROUND Reliable biomarkers of cirrhosis,hepatocellular carcinoma(HCC),or progression of chronic liver diseases are missing.In this context,Golgi protein-73(GP73)also called Golgi phosphoprotein-2,was originally defined as a resident Golgi type II transmembrane protein expressed in epithelial cells.As a result,GP73 expression was found primarily in biliary epithelial cells,with only slight detection in hepatocytes.However,in patients with acute or chronic liver diseases and especially in HCC,the expression of GP73 is significantly up-regulated in hepatocytes.So far,few studies have assessed GP73 as a diagnostic or prognostic marker of liver fibrosis and disease progression.AIM To assess serum GP73 efficacy as a diagnostic marker of cirrhosis and/or HCC or as predictor of liver disease progression.METHODS GP73 serum levels were retrospectively determined by a novel GP73 ELISA(QUANTA Lite®GP73,Inova Diagnostics,Inc.,Research Use Only)in a large cohort of 632 consecutive patients with chronic viral and non-viral liver diseases collected from two tertiary Academic centers in Larissa,Greece(n=366)and Debrecen,Hungary(n=266).Aspartate aminotransferase(AST)/Platelets(PLT)ratio index(APRI)was also calculated at the relevant time points in all patients.Two hundred and three patients had chronic hepatitis B,183 chronic hepatitis C,198 alcoholic liver disease,28 autoimmune cholestatic liver diseases,15 autoimmune hepatitis,and 5 with other liver-related disorders.The duration of follow-up was 50(57)mo[median(interquartile range)].The development of cirrhosis,liver decompensation and/or HCC during follow-up were assessed according to internationally accepted guidelines.In particular,the surveillance for the development of HCC was performed regularly with ultrasound imaging and alpha-fetoprotein(AFP)determination every 6 mo in cirrhotic and every 12 mo in non-cirrhotic patients.RESULTS Increased serum levels of GP73(>20 units)were detected at initial evaluation in 277 out of 632 patients(43.8%).GP73-seropositivity correlated at baseline with the presence of cirrhosis(96.4%vs 51.5%,P<0.001),decompensation of cirrhosis(60.3%vs 35.5%,P<0.001),presence of HCC(18.4%vs 7.9%,P<0.001)and advanced HCC stage(52.9%vs 14.8%,P=0.002).GP73 had higher diagnostic accuracy for the presence of cirrhosis compared to APRI score[Area under the curve(AUC)(95%CI):0.909(0.885-0.934)vs 0.849(0.813-0.886),P=0.003].Combination of GP73 with APRI improved further the accuracy(AUC:0.925)compared to GP73(AUC:0.909,P=0.005)or APRI alone(AUC:0.849,P<0.001).GP73 levels were significantly higher in HCC patients compared to non-HCC[22.5(29.2)vs 16(20.3)units,P<0.001)and positively associated with BCLC stage[stage 0:13.9(10.8);stage A:17.1(16.8);stage B:19.6(22.3);stage C:32.2(30.8);stage D:45.3(86.6)units,P<0.001]and tumor dimensions[very early:13.9(10.8);intermediate:19.6(18.4);advanced:29.1(33.6)units,P=0.004].However,the discriminative ability for HCC diagnosis was relatively low[AUC(95%CI):0.623(0.570-0.675)].Kaplan-Meier analysis showed that the detection of GP73 in patients with compensated cirrhosis at baseline,was prognostic of higher rates of decompensation(P=0.036),HCC development(P=0.08),and liver-related deaths(P<0.001)during follow-up.CONCLUSION GP73 alone appears efficient for detecting cirrhosis and superior to APRI determination.In combination with APRI,its diagnostic performance can be further improved.Most importantly,the simple GP73 measurement proved promising for predicting a worse outcome of patients with both viral and nonviral chronic liver diseases. | Nikolaos K Gatselis Tamás Tornai Zakera Shums Kalliopi Zachou Asterios Saitis Stella Gabeta Roger Albesa Gary L Norman Mária Papp George N Dalekos | 2020 | World Journal of Gastroenterology2020，26，34： | 21 |
| 2 | Intestinal alkaline phosphatase in the colonic mucosa of children with inflammatory bowel disease显示文摘AIM:To investigate intestinal alkaline phosphatase(iAP) in the intestinal mucosa of children with inflammatory bowel disease(IBD).METHODS:Colonic biopsy samples were taken from 15 newly diagnosed IBD patients and from 10 healthy controls.In IBD patients,specimens were obtainedboth from inflamed and non-inflamed areas.The iAP mRNA and protein expression was determined by reverse transcription-polymerase chain reaction and Western blotting analysis,respectively.Tissue localization of iAP and Toll-like receptor(TLR) 4 was investigated by immunofluorescent staining.RESULTS:The iAP protein level in the inflamed mucosa of children with Crohn's disease(CD) and ulcerative colitis(UC) was significantly decreased when compared with controls(both P < 0.05).Similarly,we found a significantly decreased level of iAP protein in the inflamed mucosa in CD compared with non-inflamed mucosa in CD(P < 0.05).In addition,the iAP protein level in inflamed colonic mucosa in patients with UC was decreased compared with non-inflamed mucosa in patients with CD(P < 0.05).iAP protein levels in the non-inflamed mucosa of patients with CD were similar to controls.iAP mRNA expression in inflamed colonic mucosa of children with CD and UC was not significantly different from that in non-inflamed colonic mucosa with CD.Expression of iAP mRNA in patients with noninflamed mucosa and in controls were similar.Co-localization of iAP with TLR4 showed intense staining with a dotted-like pattern.iAP was present in the inflamed and non-inflamed mucosa of patients with CD,UC,and in control biopsy specimens,irrespective of whether it was present in the terminal ileum or in the colon.However,the fluorescent signal of TLR4 was more pronounced in the colon compared with the terminal ileum in all groups studied.CONCLUSION:Lower than normal iAP protein levels in inflamed mucosa of IBD patients may indicate a role for iAP in inflammatory lesions in IBD.Based on our results,administration of exogenous iAP enzyme to patients with the active form of IBD may be a therapeutic option. | Kriszta Molnár dám Vannay Beáta Szebeni Nóra Fanni Bánki Erna Sziksz ron Cseh Hajnalka Gyrffy Péter László Lakatos Mária Papp András Arató Gábor Veres | 2012 | World Journal of Gastroenterology2012，18，25： | 5 |
| 3 | New serological markers in pediatric patients with inflammatory bowel disease显示文摘The spectrum of serological markers associated with inflammatory bowel disease(IBD)is rapidly growing.Due to frequently delayed or missed diagnoses,the application of non-invasive diagnostic tests for IBD,as well as differentiation between ulcerative colitis(UC)and Crohn’s disease(CD),would be useful in the pediatric population.In addition,the combination of pancreatic autoantibodies and antibodies against Saccharomyces cerevisiae antibodies/perinuclear cytoplasmic antibody(pANCA)improved the sensitivity of serological markers in pediatric patients with CD and UC.Some studies suggested that age-associated differences in the patterns of antibodies may be present,particularly in the youngest children.In CD,most patients develop stricturing or perforating complications,and a significant numberof patients undergo surgery during the disease course.Based on recent knowledge,serum antibodies are qualitatively and quantitatively associated with complicated CD behavior and CD-related surgery.Pediatric UC is characterized by extensive colitis and a high rate of colectomy.In patients with UC,high levels of antiCBir1 and pANCA are associated with the development of pouchitis after ileal pouch-anal anastomosis.Thus,serologic markers for IBD can be applied to stratify IBD patients into more homogeneous subgroups with respect to disease progression.In conclusion,identification of patients at an increased risk of rapid disease progression is of great interest,as the application of early and more aggressive pharmaceutical intervention could have the potential to alter the natural history of IBD,and reduce complications and hospitalizations. | Márta Kovács Katalin Eszter Müller Mária Papp Péter László Lakatos Mihály Cs?ndes Gábor Veres | 2014 | World Journal of Gastroenterology2014，20，17： | 4 |
| 4 | Evidence for nuclear processing of plant micro RNA and short interfering RNA precursors显示文摘 | Papp I Mette M F Aufsatz W Daxinger L Schauer S E | 2003 | Plant Physiol2003，132，： | 1 |
| 5 | The effect of preexisting adenovirus specific immunity on immune responses induced by recombinant adenovirus expressing glycoprotein D of bovine herpesvirustypel显示文摘 | Papp Z Babiuk L A Baca estrada M E | 1999 | Vaccine1999，17，： | 1 |
| 6 | Behavioural and biochemical studies of citalopram and WAY 100635 in rat chronic mild stress model显示文摘 | Papp M Nalepa I Antkiewicz-Michaluk L | 2002 | Pharmacol Biochem Behav2002，72，12： | 1 |
| 7 | A rapid engineering approach to modeling hypersonic laminar to turbulent transitional flows for 2D and 3D geometries显示文摘 | PAPP J L DASH S M | 2008 | AIAA Paper2008，2600，： | 1 |
| 8 | Simplified fast and high yielding automated synthesis of fluoroe- thylcholine for prostate cancer imaging显示文摘 | Zuhayra M Alfteimi A Papp L | 2008 | Bioorganic Medicinal Chemistry2008，16，： | 1 |
| 9 | Associations be- tween single nucleotide polymorphisms in double-stranded DNA repair pathway genes and familial breast cancer显示文摘 | SEHL M E LANGER L R PAPP J C | 2009 | Clin Can- cer Res2009，15，6： | 1 |
| 10 | Carbon dioxide hypersensitivity,hyperventilation,and panic disorder显示文摘 | Papp L A Klein D F Gorman J M | | 0，，08： | 1 |
| 11 | A new β-glucosidase gene from the zygomycete fungus Rhizomucor miehei显示文摘 | Takó M Tóth A Nagy L G Krisch J Vágv(o)lgyi C Papp T | | 0，，01： | 1 |
| 12 | Low cardiac output following open heart surgery and catecholamine therapy显示文摘 | Papp L Gombocz K Wrana G Alotti N Varr M Feiler E | 1999 | Orv Hetil1999，140，： | 1 |
| 13 | The effect of pre-existing adenovirus-specific immunity on immune responses induced by recombinant adenovirus expressing glycoprotein D of bovine herpesvirus type Ⅰ显示文摘 | Papp Z Babiuk L A Baca-Estrada M E | 1999 | Vaccine1999，17，： | 1 |
| 14 | The effect of preexisting adenovirus specific immunity on immune responses induced by recombinant adenovirus expressing glycoprotein D of bovine herpesvirus type1显示文摘 | Papp Z Babiuk L A Baca-Estrada M E | 1999 | Vaccine1999，17，： | 1 |
| 15 | Evidence for nuclear processing of plant microRNA and short interfering RNA precursors显示文摘 | Papp I Mette MF Aufsatz W Daxinger L Schauer SE Ray A van der Winden J Matzke M Matzke AJM | | 0，，： | 1 |
| 16 | Efficacy and safety of intravenous levosimendan compared with dobutamine in severe low-output heart failure (the LIDO study): a randomised double-blind trial显示文摘 | F Follath JGF Cleland H Just JGY Papp H Scholz K Peuhkurinen VP Harjola V Mitrovic M Abdalla E-P Sandell L Lehtonen | 2002 | The Lancet2002，，9328： | 1 |
| 17 | Rapid engineering approach to modeling hy- personic laminar to turbulent transitional flows 显示文摘 | Papp J L Dash S M | 2005 | Journal of Spacecraft and Rockets2005，42，5： | 1 |
| 18 | Relations of husbandst and wivesr dysphoria to marital conflict resolution strategies显示文摘 | Du Rocher Sehudlieh T D Papp L M Cummings E M | 2004 | J Fam Psychol2004，18，1： | 1 |
| 19 | The effect of preexisting adenovirus specific immunity on immune responses induced by recombinant adenovirus expressing glycoprotein D of bovine herpesvirustypel显示文摘 | Papp Z Babiuk L A Baca-Estrada M E | 1999 | Vaccine1999，17，： | 1 |
| 20 | Foreign body impaction in the sigmoid colon: A twenty euro bet显示文摘Foreign body ingestion is a common clinical problem in early childhood. However, it may occur even in adults, unknowingly. Most ingested foreign bodies entering the stomach pass through the gastrointestinal tract uneventfully. Here we report on a 13-year-old boy who presented with chronic abdominal pain, weight loss and occult gastrointestinal bleeding for 6 mo. Colonoscopy was negative; however, a ballpoint pen was impacted in the sigmoid region. Subsequently, the child admitted swallowing a pen as a 20-euro bet 6 mo previously. Crohn's disease is a chronic relaps-ing inflammatory gastrointestinal disease. It is often difficult to diagnose due to the fact that there is no single pathognomonic sign or symptom. This case is a description of an adolescent with chronic gastrointes-tinal symptoms due to a foreign body. Therefore, an ingested foreign body should be included in the differ-ential diagnostic procedure related to gastrointestinal symptoms. | Katalin E Müller András Arató Péter László Lakatos Mária Papp Gábor Veres | 2013 | World Journal of Gastroenterology2013，19，24： | 0 |
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