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| 1 | ATG16L1 and NOD2 polymorphisms enhance phagocytosis in monocytes of Crohn's disease patients显示文摘AIM:To investigate if the presence of relevant genetic polymorphisms has effect on the effectual clearance of bacteria by monocytes and granulocytes in patients with Crohn’s disease(CD).METHODS:In this study,we assessed the differential responses in phagocytosis by measuring the phagocytic activity and the percentage of active phagocytic monocytes and granulocytes in inflammatory bowel disease patients as well as healthy controls.As both autophagy related like 1(ATG16L1)and immunityrelated guanosine triphosphatase gene are autophagy genes associated with CD and more recently nucleo-tide-binding ligomerization domain-containing protein2(NOD2)has been identified as a potent inducer of autophagy we genotyped the patients for these variants and correlated this to the phagocytic reaction.The genotyping was done with restriction fragment length polymorphisms analysis and the phagocytosis was determined with the pHrodo?Escherichia coli Bioparticles Phagocytosis kit for flowcytometry.RESULTS:In this study,we demonstrate that analysis of the monocyte and granulocyte populations of patients with CD and ulcerative colitis showed a comparable phagocytic activity(ratio of mean fluorescence intensity)between the patient groups and the healthy controls.CD patients show a significantly higher phagocytic capacity(ratio mean percentage of phagocytic cells)compared to healthy controls(51.91%±2.85%vs 37.67%±7.06%,P=0.05).The extend of disease was not of influence.However,variants of ATG16L1(WT:2.03±0.19 vs homozygoot variant:4.38±0.37,P<0.009)as well as NOD2(C-ins)(heterozygous variant:42.08±2.94 vs homozygous variant:75.58±4.34(P=0.05)are associated with the phagocytic activity in patients with CD.CONCLUSION:Monocytes of CD patients show enhanced phagocytosis associated with the presence of ATG16L1 and NOD2 variants.This could be part of the pathophysiological mechanism resulting in the disease. | Simone CS Wolfkamp Caroline Verseyden Esther WM Vogels Sander Meisner Kirsten Boonstra Charlotte P Peters Pieter CF Stokkers Anje A te Velde | 2014 | World Journal of Gastroenterology2014，20，10： | 2 |
| 2 | Ligand subsititution and sulfhydryl reactivty of metallothionein 显示文摘 | Shaw CF Savs MM Petering DH | 1991 | Methods Enzymol1991，205，： | 1 |
| 3 | Can Color Doppler Sonography Aid in the Prediction of Malignancy of Thyroid Nodules显示文摘 | Mary CF Carol BB Peter MD | 2003 | J Ultrasound Med2003，22，2： | 1 |
| 4 | Basal metallothionein in tumors: widespread presence of apoprotein显示文摘 | Pattanaik A Shaw CF 3rd Petering DH | 1994 | J Inorg Biochem1994，54，2： | 1 |
| 5 | Epidemiology of Ebola (subtype Reston) virus in the Philippines, 1996 显示文摘 | Miranda ME Ksiazek TG Retuya TJ Khan AS Sanchez A Fulhorst CF Rollin PE Calaor AB Manalo DL Roces MC Dayrit MM Peters CA | 1999 | J Infect Dis1999，179，1： | 1 |
| 6 | Identification of β-lactamase-negative,ampicillin-resistant strains of Haemophilus influenzae with four methods and eight media显示文摘 | Arthur LB Peter CF Steven DB | 2001 | Antimicrob Agents Chemother2001，45，5： | 1 |
| 7 | Detection of hyperoxaemia in neonates:data from three new pulse oximeters显示文摘 | Bohnhorst B Peter CS Poets CF | 2002 | Arch Dis Child Fetal Neonatal Ed2002，87，3： | 1 |
| 8 | Identification of cell-surface markers for detecting breast cancer cells in ovarian tissue显示文摘 | Peters IT Hilders CG Sier CF | 2016 | Arch Gynecol Obstet2016，，： | 1 |
| 9 | Differential effects of diethylstilbestrol and 3,7,8-tetrachlorodibenzop dioxin on thymocyte differentiation,proliferation,and apoptosis in bcl-2 transgenic mouse fetal thymus organ culture显示文摘 | Zhi WL Nancy CF Peter JH | 2000 | Toxicol Appl pharmacol2000，68，： | 1 |
| 10 | Pulse oximeters' reliability in detecting hypoxemia and bradycardia:comparison between a conventional and two new generation oximeters显示文摘 | Bohnhorst B Peter CS Poets CF | | 0，，： | 1 |
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