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| 1 | Familial papillary carcinoma genetics criteria for diagnosis clinical features and surgical treatment显示文摘 | Petra BM Thorsten P | 2000 | World J Surg2000，24，11： | 1 |
| 2 | Arteether: risks of two-week administration in Macaca mulatta 显示文摘 | Kyle DE Gettagacamin M | 1997 | Am J Trop Med Hyg1997，56，： | 1 |
| 3 | Neurology and neuropathology of soman - induced brain injury:an overview显示文摘 | Petras JM | 1994 | J Exp Analy Behav1994，61，20： | 1 |
| 4 | Familial papillary carcinoma : genetics, criteria for diagnosis, clinical features, and surgical treatment 显示文摘 | Thomas JM Petra BM Thorsten P | 2000 | World J Surg2000，24，11： | 1 |
| 5 | Arteether: risks of two-week administration in Macaca mulatta显示文摘 | Petras JM Kyle DE | 1997 | Am J Trop Med Hyg1997，56，： | 1 |
| 6 | Exposure to sublethal blast overpressure reduces the food intake and exercise perfor- mance of rats显示文摘 | Banrnan RA Elsayed N Petras JM | 1997 | Toxicology1997，121，1： | 1 |
| 7 | Exposure to sublethal blast overpressure reduces the food intake and exercise performance of rats显示文摘 | Bauman RA Elsayed N Petras JM | 1997 | Toxicology1997，121，1： | 1 |
| 8 | Tuf m RNA rather than 16S r RNA is associated with culturable Staphylococcus aureus显示文摘AIM: To study the presence of various nucleic acids targets of Staphylococcus aureus(S. aureus) during bacterial growth and antibiotic induced killing in relation to viability.METHODS: S. aureus was cultured to log phase and spiked in Todd Hewitt(TH) broth and whole blood of healthy human volunteers. Viability of S. aureus after flucloxacillin treatment(0, 1, 3 and 6 d) was assessed by culture on bloodagar plates. DNA and RNA were isolated from 200 μL. c DNA synthesis was performed by using random primers. The presence of S. aureus DNA, r RNA, and m RNA were determined by real-time polymerase chain reaction of the 16 S r DNA and tuf gene(elongation factor Tu).RESULTS: S. aureus spiked in TH broth without antibiotics grew from day 0-6 and DNA(tuf and 16S), and 16 S r RNA remained detectable during this whole period. During flucloxacillin treatment S. aureus lost viability from day 3 onwards, while the 16 S r RNA-gene and its RNA transcripts remained detectable. DNA andr RNA can be detected in flucloxacillin treated S. aureus cultures that do not further contain culturable bacteria.However, tuf m RNA became undetectable from day 3onwards. Tuf m RNA can only be detected from samples with culturable bacteria. When spiking S. aureus in whole blood instead of broth no bacterial growth was seen, neither in the absence nor in the presence of flucloxacillin. Accordingly, no increase in DNA and RNA levels of both 16 S r DNA and the tuf gene were detected. CONCLUSION: Tuf m RNA expression is associated with culturable S. aureus and might be used to monitor antibiotic effects. | Anne JM Loonen Petra FG Wolffs Maikel de Bresser Maurice Habraken Cathrien A Bruggeman Mirjam HA Hermans Adriaan JC van den Brule | 2015 | World Journal of Clinical Infectious Diseases2015，5，4： | 0 |
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