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| 1 | Bacterial presence on flexible endoscopes vs time since disinfection显示文摘AIM To correlate the length of endoscope hang time and number of bacteria cultured prior to use.METHODS Prospectively, we cultured specimens from 19 gastroscopes, 24 colonoscopes and 5 side viewing duodenoscopes during the period of 2011 to 2015. A total of 164 results had complete data denoting date of cleansing, number of days stored and culture results. All scopes underwent initial cleaning in the endoscopy suite utilizing tap water, and then manually cleaned and flushed. High level disinfection was achieved with a Medivator~? DSD(Medivator Inc., United States) automated endoscope reprocessor following manufacturer instructions, with Glutacide~?(Pharmax Limited, Canada), a 2% glutaraldehyde solution. After disinfection, all scopes were stored in dust free, unfiltered commercial cabinets for up to 7 d. Prior to use, all scopes were sampled and plated on sheep blood agar for 48 h; the colony count was obtained from each plate. The length of endoscope hang time and bacterial load was analyzed utilizing unpaired t-tests. The overall percentage of positive and negative cultures for each type of endoscope was also calculated. RESULTS All culture results were within the acceptable range(less than 200 cfu/mL). One colonoscope cultured 80 cfu/mL after hanging for 1 d, which was the highest count. ERCP scopes cultured at most 10 cfu, this occurred after 2 and 7 d, and gastroscopes cultured 50 cfu/mL at most, at 1 d. Most cultures were negative for growth, irrespective of the length of hang time. Furthermore, all scopes, with the exception of one colonoscope which had two positive cultures(each of 10 cfu/mL), had at most one positive culture. There was no significant difference in the number of bacteria cultured after 1 d compared to 7 d when all scopes were combined(day 2: P = 0.515; day 3: P = identical; day 4: P = 0.071; day 5: P = 0.470; day 6: P = 0.584; day 7: P = 0.575). There was also no significant difference in the number of bacteria cultured after 1 day compared to 7 d for gastroscopes(day 2: P = 0.895; day 3: P = identical; day 4: P = identical; day 5: P = 0.893; day 6: P = identical; day 7: P = 0.756), colonoscopes(day 2: P = 0.489; day 4: P = 0.493; day 5: P = 0.324; day 6: P = 0.526; day 7: P = identical), or ERCP scopes(day 2: P = identical; day 7: P = 0.685). CONCLUSION There is no correlation between hang time and bacterial load. Endoscopes do not need to be reprocessed if reused within a period of 7 d. | Katlin I Mallette Peter Pieroni Sonny S Dhalla | 2018 | World Journal of Gastrointestinal Endoscopy2018，10，1： | 3 |
| 2 | Optically Active Polymers of 1-Alkynes显示文摘 | CIARDELLI F LANZILLO S PIERONI O | 1974 | Macromolecules1974，7，： | 1 |
| 3 | Ricin RCA60 evidence of its phospholipase activity显示文摘 | Helmy M Lombard S Pieroni G | 1999 | Biochem Biophys Res Commun1999，258，： | 1 |
| 4 | Potentiation of the antireac- tive, antiasthmatic effect of inhaled furosemide by inhaled lysine acetylsalicylate 显示文摘 | Bianco S VOghi A Pieroni MG | 1993 | Allergy1993，48，： | 1 |
| 5 | Cystatin C: current position and future prospects显示文摘 | Seronie-Vivien S Delanaye P Pieroni L | 2008 | Clin C hem' Lab Med2008，46，12： | 1 |
| 6 | Cystafin C:current position and future prospects 显示文摘 | Seronie-Vivien S Delanaye P Pieroni L | 2008 | Clin Chem Lab Med2008，46，12： | 1 |
| 7 | Cystatin C: current position and future prospects 显示文摘 | Seronie - Vivien S Delanaye P Pieroni L | 2008 | Clin Chem Lab Med2008，46，12： | 1 |
| 8 | Potentiation of the anti- reactive, antiasthmatie effect of inhaled furosemide by inhaled lysine aeetylsalieylate 显示文摘 | Bianco S Vaghi A Pieroni MG | 1993 | Allergy1993，48，： | 1 |
| 9 | Cardiac histological substrate in patients with clinical phenotype of Brugada syndrome显示文摘 | FRUSTACI A PRIORI S G PIERONI M | 2005 | Circulation2005，112，： | 1 |
| 10 | Hepatocyte odd protein shuttling( HOPS) is a bridging protein in the nucleophosmin-p19( Arf) network显示文摘 | Castelli M Pieroni S Brunacci C | 2013 | Oncogene2013，1132，28： | 1 |
| 11 | Protein networking:insights into global functional organization of proteomes显示文摘 | de la Fuente van Bentem S Mancosu G | 2008 | Proteomics2008，8，： | 1 |
| 12 | Protective effect of inhaled furosemide on allergen-induced early and late asthmatic reactions 显示文摘 | Bianco S Pieroni M G Refini R M | 1989 | N Engl J Med1989，321，3： | 1 |
| 13 | New insights into neuroblastoma cisplatin resistance: a comparative proteomic and meta-mining investigation显示文摘 | DAguanno S D'Alessandro A Pieroni L | 2011 | J Proteome Res2011，10，2： | 1 |
| 14 | Inhaled loop diuretices as potential new anti-asthmatic drugs显示文摘 | Pieroni MG Refini RM | 1993 | Eur Respir J1993，6，1： | 1 |
| 15 | Cardiac histological sub- strate in patients with clinical phenotype of Brugada syndrome 显示文摘 | Frustaci A Priori S G Pieroni M Chimenti C Na- politano C Rivolta I | 2005 | Circulation2005，12，： | 1 |
| 16 | Cystatin C: current position and future prospects 显示文摘 | Seronie- Vivien S Delanaye P Pieroni L | 2008 | Clinical Chemistry and Laboratory Medicine2008，46，12： | 1 |
| 17 | Ta chorta: Wild edible greens used in the Graecanic area in Calabria, southern Italy显示文摘 | Nebel S Pieroni A Heinrichs M 2006 | 2006 | Appetite2006，47，3： | 1 |
| 18 | Cystatin C: current position andfuture prospects 显示文摘 | Seronie-Vivien S Delanaye P Pieroni L | 2008 | Clin Chem Lab Med2008，46，12： | 1 |
| 19 | Cystatin C : current step and future prospects 显示文摘 | Seronie-Vivien S Delanaye P Pieroni L | 2008 | Ann Biol Clin2008，66，3： | 1 |
| 20 | Cystatin C: current position and future prospects显示文摘 | Seronie-Vivien S Delanaye P Pieroni L | 2008 | Clin Chem Lab Med2008，46，12： | 1 |
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