| 1 | Multiplex real-time RT-PCR for detecting chikungunya virus and dengue virus显示文摘Objective:To develop diagnostic test for detection chikungunya virus(CHIKV and Dengue virus (DENV) infection.Methods:We have performed a rapid,accurate laboratory confirmative method to simultaneously detect,quantify and differentiate CHIKV and DENV infection by single-step multiplex real-time RT-PCR.Results:The assay’s sensitivity was 97.65%,specificity was 92.59% and accuracy was 95.82%when compared to conventional RT-PCR.Additionally,there was no cross-reaction between CHIKV,DENV,Japanese encephalitis virus,hepatitis C,hepatitis A or hepatitis E virus.Conclusions:This rapid and reliable assay provides a means for simultaneous early diagnosis of CHIKV and DENV in a single-step reaction. | Piyathida Pongsiri Kesmanee Praianantathavorn Apiradee Theamboonlers Sunchai Payungporn Yong Poovorawan | 2012 | Asian Pacific Journal of Tropical Medicine2012，5，5： | 3 |
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