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| 1 | Predominant mucosal expression of 5-HT_(4(+h)) receptor splice variants in pig stomach and colon显示文摘AIM: To investigate cellular 5-HT4(-h/+h) receptor distribution, particularly in the epithelial layer, by laser mi-crodissection and polymerase chain reaction (PCR) in porcine gastrointestinal (GI) tissues. METHODS: A stepwise approach was used to evaluate RNA quality and to study cell-specific 5-HT4 receptor mRNA expression in the porcine gastric fundus and colon descendens. After freezing, staining and laser microdissection and pressure catapulting (LMPC), RNA quality was evaluated by the Experion automated electrophoresis system. 5-HT4 receptor and glyceral-dehyde-3-phosphate dehydrogenase (GAPDH) expressions were examined by endpoint reverse transcription (RT)-PCR in mucosal and muscle-myenteric plexus (MMP) tissue fractions, in mucosal and MMP parts of hematoxylin and eosin (HE) stained tissue sections andin microdissected patches of the epithelial and circular smooth muscle cell layer in these sections. Pig gastric fundus tissue sections were also stained immunohisto-chemically (IHC) for enterochromaffin cells (EC cells; MAB352); these cells were isolated by LMPC and examined by endpoint RT-PCR. RESULTS: After HE staining, the epithelial and circular smooth muscle cell layer of pig colon descendens and the epithelial cell layer of gastric fundus were identified morphologically and isolated by LMPC. EC cells of pig gastric fundus were successfully stained by IHC and isolated by LMPC. Freezing, HE and IHC staining, and LMPC had no influence on RNA quality. 5-HT4 recep-tor and GAPDH mRNA expressions were detected in mucosa and MMP tissue fractions, and in mucosal and MMP parts of HE stained tissue sections of pig colon descendens and gastric fundus. In the mucosa tissue fractions of both GI regions, the expression of h-exon containing receptor [5-HT4(+h) receptor] mRNA was significantly higher (P<0.01) compared to 5-HT4(-h) re-ceptor expression, and a similar trend was obtained in the mucosal part of HE stained tissue sections. Large microdissected patches of the epithelial and circular smooth muscle cell layer of pig colon descendens and of the epithelial cell layer of pig gastric fundus, also showed 5-HT4 receptor and GAPDH mRNA expression. No 5-HT4 receptor mRNA expression was detected in gastric LMPC-isolated EC cells from IHC stained tissues, which cells were positive for GAPDH. CONCLUSION: Porcine GI mucosa predominantly expresses 5-HT4(+h) receptor splice variants, suggesting their contribution to the 5-HT4 receptor-mediated mu-cosal effects of 5-HT. | Evelien KV Priem Joris H De Maeyer Mado Vandewoestyne Dieter Deforce Romain A Lefebvre | 2013 | World Journal of Gastroenterology2013，19，24： | 2 |
| 2 | Genotype and phenotype of glutathione S-transferase mu in testieular cancer patients 显示文摘 | Vistisen K Prieme H Okkels H | 1997 | Pharmacogenetics1997，7，1： | 1 |
| 3 | The Power of Altmetrics on a CV 显示文摘 | Piwowar H Priem J | 2013 | Bulletin of the Association for Information Science and Technology2013，39，4： | 1 |
| 4 | The Imperative for Open Altmetrics显示文摘 | Konkiel S Piwowar H Priem J | 2014 | Journal of Electronic Publishing2014，17，3： | 1 |
| 5 | Role of oxidative DNA damage in cancer initiation and promotion 显示文摘 | Poulsen HE Prieme H Loft S | 1998 | Eur J Cancer Prey1998，7，： | 1 |
| 6 | Triclosan affects the microbial community in simulated sewage- drain-field soil and slows down xenobiotic degradation 显示文摘 | Svenningsen H Henriksen T Priem A | 2011 | Environmental Pollution2011，159，6： | 1 |
| 7 | Correction of the iron overload defect in 13-2-microglobulin knockout mice by lactoferrin abohshes their increased susceptibihty to tuberculosis显示文摘 | Schaible U E Collins H L Priem F | 2012 | Exp Med2012，，196： | 1 |
| 8 | The power of altmetrics on a CV显示文摘 | PIWOW AR H PRIEM 1 | 2013 | Bulletin of the American Society for Information Science and Technology2013，39，4： | 1 |
| 9 | Loft S Role of oxidative DNA damage in cancer initiation and promotion显示文摘 | Poulsen H E Prieme H | 1998 | Eur J Cancer Prve1998，7，1： | 1 |
| 10 | Role of oxidative DNA damage in cancer initiation and promotion显示文摘 | Poulsen HE Prieme H Loft S | 1998 | Eur J Cancer Prev1998，7，1： | 1 |
| 11 | Top manage- ment team communication networks,environmental un- certainty,and organizational performance:A contingen- cy view显示文摘 | Liang X Ndofor H A Priem R L | 2010 | Journal of Managerial Issues2010，12，4： | 1 |
| 12 | β-trace protein in serum: a new marker of glomerular filtration rate in the creatinine-blind range 显示文摘 | Priem F Althaus H Birnbaum M | 1999 | Clin Chem1999，45，4： | 1 |
| 13 | Genotype and phenotype of glutathione S-transferase in testicular cancer patients显示文摘 | Vistisen K Prieme H Okkels H | 1997 | Pharmacogenetics1997，7，1： | 1 |
| 14 | Riding the crest of the altmetrics wave how librarians can help prepare faculty for the nert generation of research impact metrics显示文摘 | Lapinski S Piwowar H Priem J | 2013 | College & Research Libraries News2013，74，6： | 1 |
| 15 | Role of oxidative DNA damage in cancer initiation and promotion显示文摘 | POULSEN H E PRIEME H LOFT S | 1998 | European Journal of Cancer Prevention1998，7，1： | 1 |
| 16 | Process and outcome expectations for the dialectical inquiry, devil's advocacy, and consensus techniques of strategic decision making 显示文摘 | Priem R L Price K H | 1991 | Group and organization Studies1991，16，： | 1 |
| 17 | A CEO-Adviser model of strategic decision making显示文摘 | Arendt L A Priem R L Ndofor H A | 2005 | Journal of Mana- gement2005，31，5： | 1 |
| 18 | Role of oxidative DNA damage in cancer initiation and promotion显示文摘 | Poulsen HE Prieme H Loft S | 1998 | Eur J Cancer Prve1998，7，1： | 1 |
| 19 | Role of oxidative DNA damage in cancer initiation and promotion显示文摘 | Poulsen HE Prieme H Loft S | 1998 | Eur J Cancer Prey1998，7，1： | 1 |
| 20 | β-trace protein in serum:A new marker of glomerular filtration rate in the creatitine-blind range显示文摘 | Priem F Althaus H Birnbaum M | 1999 | Clin Chem1999，45，4： | 1 |
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