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| 1 | Post-translational modifications of prostaglandin-endoperoxide synthase 2 in colorectal cancer:An update显示文摘The biosynthesis of prostanoids is involved in both physiological and pathological processes. The expression of prostaglandin-endoperoxide synthase 2(PTGS2; also known as COX-2) has been traditionally associated to the onset of several pathologies, from inflammation to cardiovascular, gastrointestinal and oncologic events. For this reason, the search of selective PTGS2 inhibitors has been a focus for therapeutic interventions. In addition to the classic non-steroidal anti-inflammatory drugs, selective and specific PTGS2 inhibitors, termed coxibs, have been generated and widely used. PTGS2 activity is less restrictive in terms of substrate specificity than the homeostatic counterpart PTGS1, and it accounts for the elevated prostanoid synthesis that accompanies several pathologies. The main regulation of PTGS2 occurs at the transcription level. In addition to this, the stability of the mRNA is finely regulated through the interaction with several cytoplasmic elements, ranging from specificmicroR NAs to proteins that control mR NA degradation. Moreover, the protein has been recognized to be the substrate for several post-translational modifications that affect both the enzyme activity and the targeting for degradation via proteasomal and non-proteasomal mechanisms. Among these modifications, phosphorylation, glycosylation and covalent modifications by reactive lipidic intermediates and by free radicals associated to the proinflammatory condition appear to be the main changes. Identification of these post-translational modifications is relevant to better understand the role of PTGS2 in several pathologies and to establish a correct analysis of the potential function of this protein in diseases progress. Finally, these modifications can be used as biomarkers to establish correlations with other parameters, including the immunomodulation dependent on molecular pathological epidemiology determinants, which may provide a better frame for potential therapeutic interventions. | Rafael I Jaén Patricia Prieto Marta Casado Paloma Martín-Sanz Lisardo Boscá | 2018 | World Journal of Gastroenterology2018，24，48： | 5 |
| 2 | Interplay between post-translational cyclooxygenase-2 modifications and the metabolic and proteomic profile in a colorectal cancer cohort显示文摘BACKGROUND Colorectal cancer(CRC) is the second most common cause of cancer death worldwide. It is broadly described that cyclooxygenase-2(COX-2) is mainly overexpressed in CRC but less is known regarding post-translational modifications of this enzyme that may regulate its activity, intracellular localization and stability. Since metabolic and proteomic profile analysis is essential for cancer prognosis and diagnosis, our hypothesis is that the analysis of correlations between these specific parameters and COX-2 state in tumors of a high number of CRC patients could be useful for the understanding of the basis of this cancer in humans.AIM To analyze COX-2 regulation in colorectal cancer and to perform a detailed analysis of their metabolic and proteomic profile.METHODS Biopsies from both healthy and pathological colorectal tissues were taken under informed consent from patients during standard colonoscopy procedure in the University Hospital of Bellvitge(Barcelona, Spain) and Germans Trias i Pujol University Hospital(Campus Can Ruti)(Barcelona, Spain). Western blot analysis was used to determine COX-2 levels. Deglycosylation assays were performed in both cells and tumor samples incubating each sample with peptide N-glycosidase F(PNGase F). Prostaglandin E2(PGE2) levels were determined using a specific ELISA. 1 H high resolution magic angle spinning(HRMAS) analysis was performed using a Bruker AVIII 500 MHz spectrometer and proteomic analysis was performed in a nano-liquid chromatography-tandem mass spectrometer(nano LC-MS/MS) using a QExactive HF orbitrap MS.RESULTS Our data show that COX-2 has a differential expression profile in tumor tissue of CRC patients vs the adjacent non-tumor area, which correspond to a glycosylated and less active state of the protein. This fact was associated to a lesser PGE2 production in tumors. These results were corroborated in vitro performing deglycosylation assays in HT29 cell line where COX-2 protein profile was modified after PNGase F incubation, showing higher PGE2 levels. Moreover,HRMAS analysis indicated that tumor tissue has altered metabolic features vs non-tumor counterparts, presenting increased levels of certain metabolites such as taurine and phosphocholine and lower levels of lactate. In proteomic experiments, we detected an enlarged number of proteins in tumors that are mainly implicated in basic biological functions like mitochondrial activity,DNA/RNA processing, vesicular trafficking, metabolism, cytoskeleton and splicing.CONCLUSION In our colorectal cancer cohort, tumor tissue presents a differential COX-2 expression pattern with lower enzymatic activity that can be related to an altered metabolic and proteomic profile. | Patricia Prieto Rafael I Jaén Daniel Calle María Gómez-Serrano Estefanía Nú?ez María Fernández-Velasco Paloma Martín-Sanz Sergio Alonso Jesús Vázquez Sebastián Cerdán Miguel ángel Peinado Lisardo Boscá | 2019 | World Journal of Gastroenterology2019，25，4： | 4 |
| 3 | Somatosensory evoked potential elicited by acupoint' s stimulus显示文摘 | AbadL Alegria F Melendo JA Prieto M | 1995 | Clin Electroencepha logr1995，26，： | 1 |
| 4 | Morphology and spatial distribution of GABAergie neurons in eat primary auditory cortex (AI)显示文摘 | Prieto JJ Peterson BA Winer JA | 1994 | J Comp Neurol1994，344，： | 1 |
| 5 | Left ventricular-function after myocardial infarction, clinical and angiografic correlations显示文摘 | Cortina A Amborose JA Prieto Granada FJ | 1985 | J Am Coll Carol1985，5，： | 1 |
| 6 | Loss of MDA-7 expression with progression of melanomal显示文摘 | Ellerhorst JA Prieto VG Ekmekcioglu S | 2002 | J Clin Oncol2002，20，4： | 1 |
| 7 | Neurobiological bases of Quetiapine antidepresant effect in the bipolar disorder显示文摘 | Prieto E MicóJA Meana JJ | 2010 | Actas Esp Psiquiatr2010，38，1： | 1 |
| 8 | Solid-phase extraction and high-performance liquid chromatography applied to the determination of quinapril and its metabolite quinaprilat in urine 显示文摘 | PRIETO JA ALONSO RM JIMENEZ RM | 2001 | J Chromatogr Sci2001，39，4： | 1 |
| 9 | Somatosensory - yoked potential elicited by acupoint's stimulus 显示文摘 | Abad-Alegria F Melendo JA Prieto M Martinez T | 1995 | Clin Electroencephalogr1995，26，4： | 1 |
| 10 | Atypical cells in human cutaneous re-excision scars for melanoma express p75NGFR, C56/N-CAM and GAP-43: evidence of early Schwann cell differentiation显示文摘 | Trejo O Reed JA Prieto VG | 2002 | J Cutan Pathol2002，29，7： | 1 |
| 11 | Tumor iNOS predicts poor survival for stage III melanoma patients 显示文摘 | EKMEKCIOGLU S ELLERHORST JA PRIETO VG | 2006 | Int J Cancer2006，119，4： | 1 |
| 12 | Loss of MDA-7 expression with progression of melanoma显示文摘 | Prieto VG Ekmekcioglu S | 2002 | J Clin Oncol2002，20，4： | 1 |
| 13 | Loss of MDA-7 Expression With Progression of Melanoma显示文摘 | Ellerhorst JA Prieto VG Ekmekcioglu S | 2002 | J Clin Oncol2002，20，： | 1 |
| 14 | Thymosin modulation of regulatory T cell fuction显示文摘 | Prieto JA Schaffue JA | 1982 | Clin Immunol Immunopathol1982，23，： | 1 |
| 15 | Combined expression of Aspergillus nidulans endoxylanase X24 and Aspergillus oryzdt: (alpha) -amylase in industrial Baker’s yeasts and their use in bread making显示文摘 | MONFORT A BLASCO A PRIETO JA | 1996 | Applied and Environmental Microbiology1996，62，10： | 1 |
| 16 | Left ventricular function after myocardial infarction: clinical and angiographic correlations 显示文摘 | Cortina A Ambrose JA Prieto - Granada J | 1985 | Am Coil Cardiol1985，5，3： | 1 |
| 17 | Focal projections of cat auditory cortex to the pontine nuclei显示文摘 | Perales M Winer JA Prieto JJ | 2006 | J Comp Neurol2006，497，6： | 1 |
| 18 | Mammalian STAG3 is a co- hesin specific to sister chromatid arms inmeiosis I显示文摘 | Prieto I Suja JA Pezzi N | 2001 | Nat Cell Biol2001，3，8： | 1 |
| 19 | Thymosin modulation of regulatory T cell function显示文摘 | Mutchnick MG Prieto JA | 1982 | Clin Immuno Immunopath1982，23，： | 1 |
| 20 | Loss of MDA-7 expression with progression of melanoma显示文摘 | Ellerhorst JA Prieto VG Ekmekcioglu S | 2002 | J Clin Oncol2002，20，4： | 1 |
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