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| 1 | Determination of sperm acrosin activity for evaluation of male fertility显示文摘Aim: To investigate a simple method for assaying acrosin activity for the evaluation of male fertility. Methods: The acrosin activity of 7.5 × 10^6 sperm without seminal plasma and acrosin activity inhibitors was assayed using N-α-benzoyl-DL-arginine-p-nitroanilide (BAPNA) and detergent (Triton X-100) as substrate. Results: The acrosin activity of 60 normal fertile men (35±10μIU/10^6 sperm ) was higher than that of 168 infertile men ( 16±8μIU/10^6 sperm) (P < 0.01 ). It was indicated that there was a significant positive correlation between the acrosin activity and the sperm motility ( r≥0. 6534, P < 0.01 ) and a significant negative correlation between the sperm malformed rate and the WBC number ( r≤-0. 5426, P < 0.01 ). The temperature and time of incubation and the sperm concentration could influence the assay results. Conclusion: Acrosin activity is an important index for the evaluation of male fertility. The approach developed by the authors is a simple method for the determination of acrosin activity. | Yun-HeCUI Rui-LanZHAO QiangWANG Zi-YingZHANG | 2000 | Asian Journal of Andrology2000，2，3： | 60 |
| 2 | Discovery of hydrothermal venting community at the base of Cambrian barite in Guizhou Province,Western China: Implication for the Cambrian biological explosion显示文摘The barite deposit in the Niutitang Formation of Lower Cambrian,in Tianzhu County,Guizhou Province,western China,is a super- large barite deposit with about 2·109tons of reserves.Mineral,petrological and geochemical studies reveal that this barite deposit belongs to a hydrothermal sedimentary deposit.Microscopic observations indicate that a lot of algae,sponge spicules and tube-type fos- sils are well-preserved in this barite section,and moreover,those fossils share most characteristics of the deep-sea hydrothermal venting community in the modern Pacific.We suggest that the hydrothermal venting community was thriving in hydrothermal vent in early Cam- brian,and it is of great significance for elucidating the geological background of the‘‘Cambrian explosion’’.China Press.All rights reserved. | Ruidong Yang Huairui Wei Miao Bao Wei Wang QiangWang Xiaodong Zhang Ling Liu | 2008 | Progress in Natural Science:Materials International2008，18，1： | 10 |
| 3 | Fas ligand expression in colon cancer:A possible mechanism of tumor immune privilege显示文摘AIM: To detect the expression of Fas ligand (FasL) in colon cancer tissues and cell lines and analyze the function of FasL-expressing colon cancer cells in inducing Fas-sensitive T lymphocyte apoptosis. METHODS: Ninety surgically resected colon cancer tissues and 15 hepatic metastasis specimens were investigatedby immunohistochemical method with normal colon mucosa and colon adenoma as control. The relationship between FasL expression and pathologic features was also analyzed.FasL expression of 4 colon cancer cell lines, SW620, Lovo, LS-174T and SW1116, were detected by Western blotting assay. The function of FasL expressed on colon cancer cells was determined by coculture assay with Jurkat T lymphocytes, the apoptotic rate of which was detectedby flow cytometry assay.RESULTS: Fifty-six (62.22%) cases of all the 90 colon cancer tissues and all (100%) the liver metastasis specimens expressed FasL, significantly higher than normal colon mucosa and colonic adenoma. Higher expression of FasL was found in more advanced stage of colon cancer and in cancer tissues with lymphatic or hepatic metastasis.All the colon cancer cell lines were found to express FasL.After coculture with the SW1116 cells for 24 h with an effector: target ratio 10:1, the rate of apoptosis of Jurkat cells rose from 1.9% to 21.0%.CONCLUSION: The expression of FasL is upregulated in colon cancer and the functionally expressed FasL can induce apoptosis of Fas-expressing T lymphocytes. | WeiZhang Er-XunDing QiangWang Da-QiaoZhu JinHe Yu-LiLi Yuan-HeWang | 2005 | World Journal of Gastroenterology2005，11，23： | 6 |
| 4 | Effect of smoking on semen quality of infertile men in Shandong, China显示文摘Aim: To study the effect of smoking on the semen quality in infertile men in Shandong Province, China. Methods: Adult non-drinker males attending the infertility clinic, including 110 non-smokers and 191 smokers, were recruited for the study. Sixty-one fertile, non-smoker and non-drinker males, who had one or more children, served as the controls. The smokers were divided into subgroups according to the amount and duration of smoking. Semen parameters (semen volume and sperm density, viability, motility, and morphology) were examined and seminal plasma contents of Zn , Cu and superoxide dismutase (SOD) determined. Results: The semen volume and acidity, and the sperm density, viability and forward progression, as well as the seminal plasma contents of Zn, Cu and SOD were much lower in the medium, heavy and long-term smokers than in the non-smokers (P < 0.01). The sperm density, viability and forward progression, and the seminal plasma Zn, Cu and SOD levels were negatively correlated with the amount and duration of cigarette smoking (P < 0.01). Conclusion: Medium, heavy and long-term smoking adversely affected the semen quality in a population of men visiting the infertility clinic in Shandong, China. | Jin-PingZHANG Qing-YuMENG QiangWANG Lei-JiaZHANG Yi-LongMAO Zhuo-XiangSUN | 2000 | Asian Journal of Andrology2000，2，2： | 5 |
| 5 | Direct Generation of Intense Compression Waves in Molten Metals by Using a High Static Magnetic Field and Their Application显示文摘Compression waves propagating through molten metals are contributed to degassing accelerating reaction rate,removing exclusions from molten metals and refining solidifcation structures during metallurgical processing of materials,In the present study ,two electromagnetic methods are proposed to generate intense compression waves directly in liquid metals.One is the simultaneous imposition of a high frequency electrical current filed and a static magnetic field ;the other is that of a high frequency magnetic field and a static magnetic field ,A mathematical model based on compressible fluid dynamics and electromagnetic fileds theory has been developed to derive pressure distri-butions of the generated waves in a metal.It shows that the intensity of compression waves is proportional to that of the high frequency electromagnetic force,And the frequency is the same as that of the imposed electromagnetic force,On the basis of theoretical analyses ,pressure change in liquid gallium was examined by a pressure transducer under various conditions.The observed results approximately agreed with the predictions derived from the theoretical analyses and calculations.Moreover,the effect of the generated waves on improvement of solidification structures was also examined .It shows that the generated compression waves can refine solidification structures when they were applied to solidification process of Sn-Pb alloy ,This study indicates a new method to generate compression waves by imposing high frequency electromagnetic force locally on molten metals and this kind of compression waves can probably overcome the difficulties when waves are excited by mechanical vibration in high temperature environments. | QiangWANG SATORUKAWAI 等 | 2003 | Journal of Materials Science & Technology2003，19，1： | 5 |
| 6 | ABO blood group, hepatitis B viral infection and risk of pancreatic cancer显示文摘 | De‐ShenWang Dong‐LiangChen ChaoRen Zhi‐QiangWang Miao‐ZhenQiu Hui‐YanLuo Dong‐ShengZhang Feng‐HuaWang Yu‐HongLi Rui‐HuaXu | 2012 | Int J Cancer2012，，2： | 4 |
| 7 | Molecular regulation of Nodal signaling during mesendoderm formation显示文摘最重要的事件之一在脊椎动物 embryogenesis 期间是三细菌层,内胚叶,中层,和外胚层的形成或说明。在一系列快速的劈开以后,胚胎在迟了的 blastulation 和早 gastrulation 期间形成 mesendoderm 和外胚层。mesendoderm 然后进一步区分进中层和内胚叶。节,转变生长因素(TGF-) 的一个成员总科,由调整很多个批评抄写因素的表示在 mesendoderm 形成起一个枢轴的作用,包括像混合, GATA,短袜,和福克斯。因为节的信号 transduction 小径是描绘得好的,增加的努力被作了描出在胚胎的开发期间的节的发信号的空间与时间的调整。在这评论,我们总结描出的最近的进步节的信号紧张和持续时间的分子的规定在 mesendoderm 期间形成。 | Shi Wei QiangWang | 2018 | Acta Biochimica et Biophysica Sinica2018，50，1： | 3 |
| 8 | INHIBITORY ROLE OF TRANSCRIPTION FACTOR COUP-TFII IN EXPRESSION OF HTERT IN HELA CELLS显示文摘To clone and identify the proteins involved in regulating the transcription of hTERT and study the role of genes in both hTERT transcription and telomerase activity. Methods The full cDNA of COUP-TFII was cloned from HeLa cDNA library by hTERT promoter-based yeast one-hybrid assay and then in-frame inserted into His-tag fusion expression vector pEK318. The His-tag COUP-TFII fusion proteins were purified by Ni-NTA chromatography. The interaction of COUP-TFII with hTERT promoter in vitro was identified by electrophoretic mobility shift assay and Footprint. The role of COUP-TFII in both hTERT transcription and telomerase activity were probed through Luciferase reporter assay, Northern blot, and TRAP-PCR ELISA. Results COUP-TFII could firmly bind to the downstream E-box and the other two binding sites in hTERT promoter. Luciferase reporter assay indicated COUP-TFII could suppress hTERT promoter activity and stable introduction of COUP-TFII into HeLa cells also decreased both endogenous hTERT transcription and telomerase activity. Conclusion The human COUP-TFII can firmly bind to hTERT promoter, and inhibit telomerase activity through decreasing hTERT transcription. It will greatly facilitate understanding of telomerase regulation in normal and cancer | QiangWang Zeng-liangBai: LiXuan LinHou BoZhang | 2004 | Chinese Medical Sciences Journal2004，19，3： | 2 |
| 9 | A Facile and Efficient Oxidation of α,β-Unsaturated Alcohols with Manganese Dioxide in Ionic Liquids under Mild Conditions显示文摘The oxidation of a,b-unsaturated primary and secondary alcohols to corresponding aldehydes and ketones by manganese dioxide in ionic liquids as a safe recyclable and accelerative reaction medium under mild conditions are described. The rate of the oxidation reaction is faster and the yield is higher than that with conventional procedures. | WeiLiangBAO QiangWANG YunFaZHENG | 2004 | Chinese Chemical Letters2004，15，9： | 2 |
| 10 | Evidence for the Natural Toxins from the Mushroom Trogia venenata as a Cause of Sudden Unexpected Death in Yunnan Province, China显示文摘 | Zhong‐YuZhou Guo‐QingShi RobertFontaine KunWei TaoFeng FangWang Gang‐QiangWang YanQu Zheng‐HuiLi Ze‐JunDong Hua‐JieZhu Zhu‐LiangYang GuangZeng Ji‐KaiLiu | 2012 | Angew Chem Int Ed2012，，10： | 1 |
| 11 | The Complete Genome Sequence of Two Isolates of Southern rice black‐streaked dwarf virus, a New Member of the Genus Fijivirus显示文摘 | QiangWang JianYang Guo‐HuiZhou Heng‐MuZhang Jian‐PingChen Michael J.Adams | 2010 | Journal of Phytopathology (鈥?)2010，，11： | 1 |
| 12 | A meta‐analytic examination of the relationship between job satisfaction and subjective well‐being显示文摘 | Nathan A.Bowling Kevin J.Eschleman QiangWang | 2011 | Journal of Occupational and Organizational Psychology2011，，4： | 1 |
| 13 | Fabrication of High‐Strength Continuous Zirconia Fibers and Their Formation Mechanism Study显示文摘 | He‐YiLiu Xian‐QinHou Xin‐QiangWang Yan‐LingWang DongXu ChenWang WeiDu Meng‐KaiLü Duo‐RongYuan | 2005 | Journal of the American Ceramic Society2005，，12： | 1 |
| 14 | The Complete Genome Sequence of Two Isolates of Southern rice black‐streaked dwarf virus, a New Member of the Genus Fijivirus显示文摘 | QiangWang JianYang Guo‐HuiZhou Heng‐MuZhang Jian‐PingChen Michael J.Adams | 2010 | 2010 (11‐)2010，，11： | 1 |
| 15 | Polarization Orderings and New Classes of Polarization Indices显示文摘 | You‐QiangWang Kai‐YuenTsui | 2002 | Journal of Public Economic Theory2002，，3： | 1 |
| 16 | Evidence for the Natural Toxins from the Mushroom Trogia venenata as a Cause of Sudden Unexpected Death in Yunnan Province, China显示文摘 | Zhong‐YuZhou Guo‐QingShi RobertFontaine KunWei TaoFeng FangWang Gang‐QiangWang YanQu Zheng‐HuiLi Ze‐JunDong Hua‐JieZhu Zhu‐LiangYang GuangZeng Ji‐KaiLiu | 2012 | Angew Chem Int Ed2012，，10： | 1 |
| 17 | The Complete Genome Sequence of Two Isolates of Southern rice black‐streaked dwarf virus, a New Member of the Genus Fijivirus显示文摘 | QiangWang JianYang Guo‐HuiZhou Heng‐MuZhang Jian‐PingChen Michael J.Adams | 2010 | Journal of Phytopathology (鈥?)2010，，11： | 1 |
| 18 | Diagnosis and Surgical Treatment Parathyroid Adenoma (24 Case Report)显示文摘OBJECTIVE To summarize the experience in diagnosing and treating parathyroid adenoma.METHODS Twenty-four patients were diagnosed with parathyroid adenoma and received parathyroidectomy in our hospital. Sixteen of them presented with hyperparathyroidism. The patients received ultrasounography, CT or ^99mTc-MIBI to locate the tumor site. Serum concentrations of PTH and calcium were checked before the operation. All operations were performed under general anesthesia. The adenomas were resected and the four glands explored.RESULTS All of the patients were cured and there was no mortality in our group. The symptoms of hyperparathyroidism remitted to various degrees after the operation. PTH dropped to the normal range 2 days after operation.Serum calcium concentrations declined to different levels from the first day after operation. Seven patients developed hypocalcemia post-operation but recovered by injection of calcium gluconate. Only one of the patients with parathyroid adenoma recurred 2 years after the operation and was found to have malignancy of the parathyroid adenoma.CONCLUSION Not all the patients with parathyroid adenoma had clinical manifestations. The CT and ^99mTc-MIBI were more accurate than ultrasounography in locating the adenoma. The four glands should be explored during the operation. Protecting the recurrent laryngeal nerve from being injuried and maintaning secure hemastasis were most important. | WeiZhang JunchuZhang DaqiaoZhu ZhiqianHu QiangWang | 2005 | Chinese Journal of Clinical Oncology2005，2，1： | 0 |
| 19 | 一种基于学习的复杂非刚体运动跟踪方法显示文摘复杂非刚体运动的跟踪是计算机视觉中的一个重要研究问题,在人机交互、运动合成与识别等领域有着广泛的应用。复杂非刚体运动跟踪的困难在于运动的自由度比较大,运动状态向量是一个高维的向量,而直接在高维空间中跟踪或求解图像中物体的运动状态向量是一个约束不充分、并因此而难以解决的问题。本文的基本思想是采用机器学习的方法来学习复杂非刚体运动的状态表示及其动态模型,从而将一个约束不充分的跟踪问题转化成一个约束充分的跟踪问题。 | QiangWang Hai-ZhouAi Guang-YouXu | 2004 | Journal of Computer Science & Technology2004，19，C00： | 0 |
| 20 | Adjustable volume and loading release of shape memory polymer microcapsules显示文摘Research on microcapsules has been conducted in recent years given trends in miniaturization and novel functionalization.In this work,we designed and prepared a series of unique shape memory polyurethane(SMPU)microcapsules with stimuli-responsive func-tions.The microcapsule has a core-shell structure in which the surface morphology can be adjusted,and it has a certain loadbearing capacity.In addition,the SMPU microcapsule has a stimuliresponsive function for shape memory and solvent response.The temperature of its shape recovery is approximately body tempera-ture,and it can swell to rupture under the stimulation of organic solvents.Thus,the SMPU microcapsule has potential applications in biomedical fields,such as drug release. | Fenghua Zhang Lu Wang Qiangwang Geng Yanju Liu Jinsong Leng Stoyan K.Smoukov | 2023 | International Journal of Smart and Nano Materials2023，14，1： | 0 |
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