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| 1 | Dynamic Changes of Tobacco-growing Soil Nutrients and Enzymes After Application of Amelioration Materials显示文摘[Objectives]This study was conducted to clarify the improvement effects of different soil amelioration materials such as lime,green manure and bio-organic fertilizer on acidic tobacco-planting soil.[Methods]The dynamic changes of soil pH,soil nutrients and enzyme activity were studied by applying lime,lime+green fertilizer,and lime+green fertilizer+biological organic fertilizer.[Results]①After the application of amelioration materials,the soil pH and available phosphorus content of tobacco-growing soil showed a stepwise change of first increasing and then decreasing,and became stable at 60 d after tobacco transplanting;the soil organic matter,alkali-hydrolyzable nitrogen and available potassium content showed a gradual change trend of'high-low-high';and the soil invertase and urease activity showed a gradual change trend of'low-high-low'.②To 90 d after tobacco transplanting,the application of amelioration materials increased soil pH by 1.29-1.62 units,and increased organic matter content by 15.21%-20.86%,alkali-hydrolyzable nitrogen content by 6.83%-18.17%,available phosphorus content by 54.15%-217.85%,rapidly available potassium content by 11.42%-30.86%,soil invertase activity by 70.09%-18.93%,and soil urease activity by 64.07%-130.47%.③The combination of lime+green manure+alkaline microbial organic fertilizer had the best effect on the improvement of acidic tobacco-growing soil,and the effect of lime+green manure+acid microbial organic fertilizer on acidic soil was the second.[Conclusions]When applying lime,green fertilizer and alkaline bio-organic fertilizer should be applied to achieve sustainable improvement of strongly acidic soil. | Zhanghong CAO Gexuan SU Yongjun LIU Shuguang PENG Yongsheng DENG Jin CHEN Deyuan PENG Zhenghua WANG Jingjing WU Qianping YAN Xiaohua DENG | 2020 | Agricultural Biotechnology2020，9，4： | 1 |
| 2 | Enantioselective synthesis of tetrasubstituted allenes via addition/arylation tandem reaction of 2-activated 1,3-enynes显示文摘We report the development of a new class of multifunctional chiral guanidine/Pd(0)catalyst system for 1,4-addition/arylation tandem reaction.A variety of tetra-substituted allenes were readily accessible from three-component“one-pot”transformations of acyclic or cyclic 2-activated 1,3-enynes,malonates and halobenzenes under mild reaction conditions.High levels of yield and enantioselectivity were achieved in the construction of stereogenic center and axis using readily available acyclic guanidineamides.The mechanistic studies suggest that the guanidine/Pd(0)collaboration has obvious synergism to both base-dominated conjugate addition,and Pd(0)-dominated Heck-type reaction. | Yongyan Zhang Jin Wu Lichao Ning Qianping Chen Xiaoming Feng Xiaohua Liu | 2023 | Science China Chemistry2023，66，2： | 0 |
| 3 | HBXIP contributes to radioresistance through NF-κB-mediated expression of XIAP in breast cancer显示文摘Objective:Hepatitis B X-interacting protein(HBXIP)plays an important role in breast tumorigenesis,tumor growth and metastasis,but its functional contribution in radioresistance remains poorly understood.As radiotherapy served as an essential adjuvant treatment,uncovering the role of HBXIP as well as its downstream molecular XIAP in radioresistance could benefit for the development of individual therapy strategy.Methods:Immunohistochemistry of 42 breast cancer tissue samples and Western blot analysis of proteins from MCF-7 and MDA-MB-231 cells exposed to fractioned doses(γ-rays)were used to identify the expression of HBXIP/XIAP in breast cancer.To verify the radioresistance effects and potential mechanism,the cells were treated with designed pCMV and siRNA of targeting genes,and then measured with MTT assay,clonogenic survival assay and flow cytometry.Furthermore,a subcutaneous xenotransplanted tumor model of breast cancer was established in nude mice to validate the radioresistization effect of HBXIP in vivo.Results:HBXIP and XIAP expression levels in breast cancer tissues were positively correlated with chemoradiotherapy resistance of breast cancer.Overexpression of HBXIP could desensitize MCF-7 and MDA-MB-231 cells to irradiation by inhibiting radiation-induced cell apoptosis,and knockdown of HBXIP in these cells had the converse response.Moreover,up-regulation of HBXIP resulted in the increase of XIAP and NF-κB levels in vitro and in vivo,while down-regulation of HBXIP led to the opposite effects.In addition,inhibition of XIAP and NF-κB abrogated the HBXIP overexpression induced radioresistization and increased cell apoptosis(25.8%augment for siRNA XIAP and 28.1%for NF-κB in MDA-MB-231 cells;25.4%augment for siRNA XIAP and 27.2%for NF–κB in MCF-7 cells).Conclusions:HBXIP enhances radioresistance of human breast cancer cells via upregulating XIAP,and targeting the HBXIP–NF–κB-XIAP pathway may be a potentially effective strategy to enhance the efficacy of radiotherapy for human breast cancer. | Xiaofei Chu Wang Zheng Qianping Chen Chen Wang Saijun Fan Chunlin Shao | 2021 | Radiation Medicine and Protection2021，2，2： | 0 |
| 4 | Mechanically conditioned cell sheets cultured on thermo-responsive surfaces promote bone regeneration显示文摘Cell sheet-based scaffold-free technology holds promise for tissue engineering applications and has been extensively explored during the past decades.However,efficient harvest and handling of cell sheets remain challenging,including insufficient extracellular matrix content and poor mechanical strength.Mechanical loading has been widely used to enhance extracellular matrix production in a variety of cell types.However,currently,there are no effective ways to apply mechanical loading to cell sheets.In this study,we prepared thermo-responsive elastomer substrates by grafting poly(N-isopropyl acrylamide)(PNIPAAm)to poly(dimethylsiloxane)(PDMS)surfaces.The effect of PNIPAAm grafting yields on cell behaviours was investigated to optimize surfaces suitable for cell sheet culturing and harvesting.Subsequently,MC3T3-E1 cells were cultured on the PDMS-g-PNIPAAm substrates under mechanical stimulation by cyclically stretching the substrates.Upon maturation,the cell sheets were harvested by lowering the temperature.We found that the extracellular matrix content and thickness of cell sheet were markedly elevated upon appropriate mechanical conditioning.Reverse transcription quantitative polymerase chain reaction and Western blot analyses further confirmed that the expression of osteogenic-specific genes and major matrix components were up-regulated.After implantation into the critical-sized calvarial defects of mice,the mechanically conditioned cell sheets significantly promoted new bone formation.Findings from this study reveal that thermo-responsive elastomer,together with mechanical conditioning,can potentially be applied to prepare high-quality cell sheets for bone tissue engineering. | Gen Wang Zhangqin Yuan Li Yu Yingkang Yu Pinghui Zhou Genglei Chu Huan Wang Qianping Guo Caihong Zhu Fengxuan Han Song Chen Bin Li | 2023 | Biomaterials Translational2023，4，1： | 0 |
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