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| 1 | Origin and phylogenetic analysis of Tibetan Mastiff based on the mitochondrial DNA sequence显示文摘At present,the Tibetan Mastiff is the oldest and most ferocious dog in the world. However,the origin of the Tibetan Mastiff and its phylogenetic relationship with other large breed dogs such as Saint Bernard are unclear. In this study,the primers were designed accord-ing to the mitochondrial genome sequence of the domestic dog,and the 2,525 bp mitochondrial sequence,containing the whole sequence of Cytochrome b,tRNA-Thr,tRNA-Pro,and control region of the Tibetan Mastiff,was obtained. Using grey wolves and coyotes as out-groups,the Tibetan Mastiff and 12 breeds of domestic dogs were analyzed in phylogenesis. Tibetan Mastiff,domestic dog breeds,and grey wolves were clustered into a group and coyotes were clustered in a group separately. This indicated that the Tibetan Mastiff and the other domestic dogs originated from the grey wolf,and the Tibetan Mastiff belonged to Carnivora,Canidae,Canis,Canis lupus,Canis lupus familiaris on the animal taxonomy. In domestic dogs,the middle and small breed dogs were clustered at first; German Sheepdog,Swedish Elkhound,and Black Russian Terrier were clustered into one group,and the Tibetan Mastiff,Old English Sheepdog,Leonberger,and Saint Bernard were clustered in another group. This confirmed the viewpoint that many of the famous large breed dogs worldwide such as Saint Bernard possibly had the blood lineage of the Tibetan Mastiff,based on the molecular data. According to the substitution rate,we concluded that the approximate divergence time between Tibetan Mastiff and grey wolf was 58,000 years before the present (YBP),and the approximate divergence time between other domestic dogs and grey wolf was 42,000 YBP,demonstrating that the time of origin of the Tibetan Mastiff was earlier than that of the other domestic dogs. | Qifa Li Zhenshan Liu Yinxia Li Xingbo Zhao Liyan Dong Zengxiang Pan Yuanrong Sun Ning Li Yinxue Xu Zhuang Xie | 2008 | Journal of Genetics and Genomics2008，35，6： | 15 |
| 2 | Current Advances in Epigenetic Modification and Alteration during Mammalian Ovarian Folliculogenesis显示文摘During the growth and development of mammalian ovarian follicles, the activation and deactivation of mass genes are under the synergistic control of diverse modifiers through genetic and epigenetic events. Many factors regulate gene activity and functions through epigenetic modification without altering the DNA sequence, and the common mechanisms may include but are not limited to: DNA methylation, histone modifications (e.g., acetylation, deacetylation, phosphorylation, methylation, and ubiquitination), and RNA- associated silencing of gene expression by noncoding RNA. Over the past decade, substantial progress has been achieved in studies involving the epigenetic alterations during mammalian germ cell development. A number of candidate regulatory factors have been identified. This review focuses on the current available information of epigenetic alterations (e.g., DNA methylation, histone modification, noncoding-RNA-mediated regulation) during mammalian folliculogenesis and recounts when and how epigenetic patterns are differen- tially established, maintained, or altered in this process. Based on different types of epigenetic regulation, our review follows the temporal progression of events during ovarian folliculogenesis and describes the epigenetic changes and their contributions to germ cellespecific functions at each stage (i.e., primordial folliculogenesis (follicle formation), follicle maturation, and follicular atresia). | Zengxiang Pan Jinbi Zhang Qifa Li Yinxia Li Fangxiong Shi Zhuang Xie Honglin Liu | 2012 | Journal of Genetics and Genomics2012，39，3： | 2 |
| 3 | Suppression of starch synthase I(SSI) by RNA interference alters starch biosynthesis and amylopectin chain distribution in rice plants subjected to high temperature显示文摘Based on known cDNAs of rice starch synthase isoforms,we constructed dsRNA interference vectors for starch synthase I(SSI)to produce transgenic plants containing starch with a moderately high amylose content.We investigated the effect of SSI suppression on grain quality traits,starch biosynthesis,and amylopectin chain distribution in rice plants exposed to two different temperature regimes.The activities and transcripts of BEs,DBEs,and other SS isoforms were further investigated to clarify the effect of SSI suppression on these key enzymes and their specific isoforms under different temperature treatments.Suppression of SSI by RNAi altered grain starch component and amylopectin chain distribution,but it exerted only a slight effect on total starch content(%)and accumulation amount(mg kernel?1)and on starch granule morphology and particle size distribution.Under normal temperature(NT),insignificant differences in kernel weight,chalky kernel proportion,chalky degree,and starch granule morphology between SSI-RNAi line and its wild type(WT)were observed.However,amylose content(AC)level and granule-bound starch synthase(GBSS)activity in rice endosperms were markedly increased by SSI-RNAi suppression.The chalky kernel proportion and chalky degree of SSIRNAi lines were significantly higher than those of WT under high temperature(HT)exposure at filling stage.Inhibition of SSI by RNAi affected amylopectin chain distribution and raised starch gelatinization temperature(GT)in two ways:directly from the SSI deficiency itself and indirectly by reducing BEIIb amounts in an SSI-deficient background.The deficiency of SSI expression led to an alteration in the susceptibility of grain chalkiness occurrence and starch gelatinization temperature to HT exposure,owing to a pleiotropic effect of SSI deficiency on the expression of other genes associated with starch biosynthesis. | Qian Zhao Xiaoxia Du Zhanyu Han Yu Ye Gang Pan Muhammad-Asad-Ullah Asad Qifa Zhou Fangmin Cheng | 2019 | The Crop Journal2019，7，5： | 2 |
| 4 | NORHA,a novel follicular atresia-related lncRNA,promotes porcine granulosa cell apoptosis via the miR-183-96-182 cluster and FoxO1 axis显示文摘Background:Follicular atresia has been shown to be strongly associated with a low follicle utilization rate and female infertility,which are regulated by many factors such as microRNAs(miRNAs),which constitute a class of noncoding RNAs(ncRNAs).However,little is known about long noncoding RNAs(lncRNAs),which constitute another ncRNA family that regulate follicular atresia.Results:A total of 77 differentially expressed lncRNAs,including 67 upregulated and 10 downregulated lncRNAs,were identified in early atretic follicles compared to healthy follicles by RNA-Sequencing.We characterized a noncoding RNA that was highly expressed in atretic follicles(NORHA).As an intergenic lncRNA,NORHA was one of the upregulated lncRNAs identified in the atretic follicles.To determine NORHA function,RT-PCR,flow cytometry and western blotting were performed,and the results showed that NORHA was involved in follicular atresia by influencing GC apoptosis with or without oxidative stress.To determine the mechanism of action,bioinformatics analysis,luciferase reporter assay and RNA immunoprecipitation assay were performed,and the results showed that NORHA acted as a‘sponge’,that directly bound to the miR-183-96-182 cluster,and thus prevented its targeted inhibition of FoxO1,a major sensor and effector of oxidative stress.Conclusions:We provide a comprehensive perspective of lncRNA regulation of follicular atresia,and demonstrate that NORHA,a novel lncRNA related to follicular atresia,induces GC apoptosis by influencing the activities of the miR-183-96-182 cluster and FoxO1 axis. | Wang Yao Zengxiang Pan Xing Du Jinbi Zhang Honglin Liu Qifa Li | 2022 | Journal of Animal Science and Biotechnology2022，13，2： | 2 |
| 5 | Molecular cloning, gene expression and methylation status analysis of PIWIL1 in cattle-yaks and the parental generation显示文摘 | Yao Gu Qifa Li Zengxiang Pan Minggui Li Hua Luo Zhuang Xie | 2013 | Animal Reproduction Science2013，，： | 1 |
| 6 | Cathodic corrosion as a facile and universal method for the preparation of supported metal single atoms显示文摘Top-down strategy has been generally adopted for preparation of metal single atom catalysts(SACs)due to the simplified synthetic process,metal economics,and scalability characteristics.Herein,we propose a general top-down route to convert metal nanoparticles into uniformly dispersed metal single atoms in mild electrochemical environment via a facile cathodic corrosion process.Within the synthetic process,Pt nanoparticles precursors are transformed into migrating Pt single atoms(Pt1)driven by a high negative potential;and subsequently these mobile Pt atoms are trapped and stabilized by N coordination sites of N-doped carbon paper(NCP).The as-prepared Pt1/NCP electrodes exhibit a superior catalytic activity toward hydrogen evolution reaction(HER)with a low overpotential of 0.022 V at 10 mA/cm^(2)and a low Tafel slope of 28.5 mV/dec as well as a long-term durability.Notably,the proposed electrochemical atomic migration strategy shows a promising generality for fabricating other metal single atoms(e.g.,Pd,Ir,Cu),which may open a new avenue for metallic SACs preparation. | Rui Li Jingsong Xu Qingkai Zhao Wensheng Ren Rongguang Zeng Qifa Pan Xiayan Yan Jingwen Ba Tao Tang Wenhua Luo | 2022 | Nano Research2022，15，3： | 0 |
| 7 | Research on the Construction of a Fully-participatory Extracurricular Learning Platform with Multiple Linkage Effects显示文摘In the existing research at home and abroad,the construction of extracurricular learning platform is still only focused on solving the problems of curriculum learning itself.At the same time,there are no cases of multiple linkage effects,including integrating alumni resources,promoting the construction of alumni association,promoting students'internship and employment,strengthening ties with enterprises and so on.On the basis of the original function of the alumni management system,this paper expands the sections and adds the main body of students to enrich the functions of the platform.This paper constructs a fully-participatory extracurricular learning platform with multiple linkage effect,which provides a reference for other majors inside and outside the school to establish extracurricular learning platform. | Jiasen PAN Xuewei ZHU Qifa LI Jiang YU | 2020 | Asian Agricultural Research2020，12，5： | 0 |
| 8 | Platinum single atom catalysts for hydrogen isotope separation during hydrogen evolution reaction显示文摘Electrolysis of water is widely used for hydrogen isotope separation and the development of hydrogen evolution reaction(HER)catalysts with high selectivity and activity is of key importance.Herein,we propose single atom catalysts(SACs)as promising catalysts for efficient hydrogen isotope separation.Pt SACs and Pt nanoparticles(NPs)have been fabricated on nanoarray-structured nitrogen-doped graphite foil(NGF)substrate by a polyol reduction method.The as prepared Pt1/NGF electrode exhibits high activity and selectivity toward HER with a low overpotential of 0.022 V at 10 mA·cm^(-2) and a high separation factor of 6.83 for hydrogen and deuterium separation,much better than Pt NPs counterpart.Density functional theory(DFT)calculations ascribe the high activity and selectivity to the constructed Pt-N_(2)C_(2) structure.This work develops a new opportunity for the design and application of high-efficiency and stable SACs toward hydrogen isotope separation by electrolysis of water. | Jingsong Xu Rui Li Xiayan Yan Qingkai Zhao Rongguang Zeng Jingwen Ba Qifa Pan Xin Xiang Daqiao Meng | 2022 | Nano Research2022，15，5： | 0 |
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