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    题名 作者 年代 出处 被引量
1Distinct patterns of pigment suppression are produced by allelic sense and antisense chalcone synthase transgenes in Petunia flowers显示文摘Qiudeng Que Wang HY Richard A 1998The Plant Journal1998,13,3:1
2Chalcone synthase cosuppression phenotypes in petunia flowers: comparison of sense vs. antisense constructs and single-copy vs. complex T-DNA sequences显示文摘Richard A. Jorgensen Paul D. Cluster James English Qiudeng Que Carolyn A. Napoli 1996Plant Molecular Biology1996,,5:1
3Chalcone synthase cosuppression phenotypes in petunia flowers: comparison of sense vs. antisense constructs and single-copy vs. complex T-DNA sequences显示文摘Richard A. Jorgensen Paul D. Cluster James English Qiudeng Que Carolyn A. Napoli 1996Plant Molecular Biology1996,,5:1
4Chalcone synthase cosuppression phenotypes in petunia flowers: comparison of sense vs. antisense constructs and single-copy vs. complex T-DNA sequences显示文摘Richard A. Jorgensen Paul D. Cluster James English Qiudeng Que Carolyn A. Napoli 1996Plant Molecular Biology1996,,5:1
5Chalcone synthase cosuppression phenotypes in petunia flowers: comparison of sense vs. antisense constructs and single-copy vs. complex T-DNA sequences显示文摘Richard A. Jorgensen Paul D. Cluster James English Qiudeng Que Carolyn A. Napoli 1996Plant Molecular Biology1996,,5:1
6Elite,transformable haploid inducers in maize显示文摘The introduction of alleles into commercial crop breeding pipelines is both time consuming and costly.Two technologies that are disrupting traditional breeding processes are doubled haploid(DH)breeding and genome editing(GE).Recently,these techniques were combined into a GE trait delivery system called HI-Edit(Haploid Inducer-Edit).In HI-Edit,the pollen of a haploid inducer line is reprogrammed to deliver GE traits to any variety,obviating recurrent selection.For HI-Edit to operate at scale,an efficient transformable HI line is needed,but most maize varieties are recalcitrant to transformation,and haploid inducers are especially difficult to transform given their aberrant reproductive behaviors.Leveraging marker assisted selection and a three-tiered testing scheme,we report the development of new Iodent and Stiff Stalk maize germplasm that are transformable,have high haploid induction rates,and exhibit a robust,genetically-dominant anthocyanin native trait that may be used for rapid haploid identification.We show that transformation of these elite‘‘HI-Edit”lines is enhanced using the BABYBOOM and WUSCHEL morphogenetic factors.Finally,we evaluate the HI-Edit performance of one of the lines against both Stiff Stalk and non-Stiff Stalk testers.The strategy and results of this study should facilitate the development of commercially scalable HI-Edit systems in diverse crops.Brent Delzer Dawei Liang David Szwerdszarf Isadora Rodriguez Gonzalo Mardones Sivamani Elumalai Francine Johnson Samson Nalapalli Rachel Egger Erin Burch Kerry Meier Juan Wei Xiujuan Zhang Huaping Gui Huaibing Jin Huan Guo Kun Yu Yubo Liu Becky Breitinger Ana Poets Jason Nichols Wan Shi David Skibbe Qiudeng Que Timothy Kelliher 2024The Crop Journal2024,12,1:0
7CRISPR-Cas12a base editors confer efficient multiplexed genome editing in rice显示文摘Dear Editor,Many Cas9-derived base editors have been developed for precise C-to-T and A-to-G base editing in plants(Molla et al.,2021).They are typically based on a SpCas9 nickase or its engineered variants with altered protospacer adjacent motif(PAM)requirements(Molla et al.,2021).CRISPR-Cas12a enables highly efficient multiplexed genome editing in plants,and its T-rich PAM preference complements the G-rich PAM requirement of SpCas9 in genome targeting(Zhang et al.,2019,2021).Because of the lack of an efficient Cas12a nickase,it has been challenging to develop efficient Cas12a base editors.Nevertheless,Cas12a cytosine base editors(CBEs)and adenine base editors(ABEs)have been developed in mammalian cells(Li et al.,2018;Kleinstiver et al.,2019)with low DNA damage(Wang et al.,2020)because deactivated Cas12a(dCas12a)was used.However,efficient dCas12a base editors are yet to be developed in plants.Yanhao Cheng Yingxiao Zhang Gen Li Hong Fang Simon Sretenovic Avery Fan Jiang Li Jianping Xu Qiudeng Que Yiping Qi 2023Plant Communications2023,4,4:0
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