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| 1 | The chimeric genes in the hybrid lineage of Carassius auratus cuvieri(♀)×Carassius auratus red var.(♂)显示文摘Hybridization can combine the genomes of different strains or species, which leads to changes of genotype and phenotype in the hybrids. In this study, we aimed to investigate the genetic variations of hybrids(WR-F1 and WR-F2) derived from the intraspecific hybridization of white crucian carp(Carassius auratus cuvieri, WCC, ♀) and red crucian carp(Carassius auratus red var., RCC, ♂). Here, we compared the orthologous genes in the liver transcriptomes of hybrids with those of WCC and RCC, and classified the orthologous genes into eight gene patterns within three categories(chimera, mutant, and biparental origin genes).The results revealed 19.04%, 4.17% chimeric genes and 6.90%, 5.05% mutations of orthologous genes in WR-F1 and WR-F2 respectively. Seventeen of twenty-three characterized genes(77%) were confirmed to be the chimeras at the genomic DNA level.The GO classification discovered that some chimeric and mutant genes were related to metabolic process, immune system and developmental process in WR-F1. Our results provide the new evidence that hybridization can combine the parental genomes,leading to changes in the genotype of the resultant hybrids. This is the first report on the formation of chimeric genes from fish intraspecific hybridization, which is potentially interesting from the context of both evolution and the genetic breeding of fish. | Qingfeng Liu Yanhua Qi Qiuli Liang Xiujuan Xu Fangzhou Hu Jing Wang Jun Xiao Shi Wang Wuhui Li Min Tao Qinbo Qin Rurong Zhao Zhanzhou Yao Shaojun Liu | 2018 | Science China(Life Sciences)2018，61，9： | 6 |
| 2 | Targeted disruption of tyrosinase causes melanin reduction in Carassius auratus cuvieri and its hybrid progeny显示文摘The white crucian carp(Carassius auratus cuvieri,WCC) not only is one of the most economically important fish in Asia,characterized by strong reproductive ability and rapid growth rates,but also represents a good germplasm to produce hybrid progenies with heterosis.Gene knockout technique provides a safe and acceptant way for fish breeding.Achieving gene knockout in WCC and its hybrid progeny will be of great importance for both genetic studies and hybridization breeding.Tyrosinase(TYR) is a key enzyme in melanin synthesis.Depletion of tyr in zebrafish and mice results in mosaic pigmentation or total albinism.Here,we successfully used CRISPR-Cas9 to target tyr in WCC and its hybrid progeny(WR) derived from the cross of WCC(♀) and red crucian carp(Carassius auratus red var.,RCC,♂).The level of TYR protein was significantly reduced in mutant WCC.Both the mutant WCC and the mutant WR showed different degrees of melanin reduction compared with the wild-type sibling control fish,resulting from different mutation efficiency ranging from 60% to 90%.In addition,the transcriptional expression profiles of a series of pivotal pigment synthesis genes,i.e.tyrp1,mitfa,mitfb,dct and sox10,were down-regulated in tyr-CRISPR WCC,which ultimately caused a reduction in melanin synthesis.These results demonstrated that tyr plays a key role in melanin synthesis in WCC and WR,and CRISPR-Cas9 is an effective tool for modifying the genome of economical fish.Furthermore,the tyr-CRISPR models could be valuable in understanding fundamental mechanisms of pigment formation in non-model fish. | Qingfeng Liu Yanhua Qi Qiuli Liang Jia Song Junmei Liu Wuhui Li Yuqin Shu Min Tao Chun Zhang Qinbo Qin Jing Wang Shaojun Liu | 2019 | Science China(Life Sciences)2019，62，9： | 4 |
| 3 | Brood sex ratio in the Yellow-bellied Prinia(Prinia flaviventris)显示文摘Background:The adjustment of sex ratios in birds can occur at the egg and nestling stages. Previous studies showed that the sex ratio was affected by environmental factors and parental condition; it may result in seasonal and ecosystem differences.Methods:In this study, the brood sex ratio of the Yellow-bellied Prinia(Prinia flaviventris) in the Nonggang area, Guangxi, southwestern China, was investigated during the breeding season from May to June in 2013 using PCR amplification from whole-genome DNA extracted from blood samples. A total of 31 nests of Yellow-bellied Prinia, including 132 brood fledglings and 31 pairs, were sampled.Results:The results showed that the brood sex ratio of the Yellow-bellied Prinia was 1:1, and sex ratios of different nests were evenly distributed within the study area. No significant relationship was found between parental quality and nest characteristics with the brood sex ratio.Conclusions:The present study indicated that no brood sex ratio bias in the Yellow-bellied Prinia highlighted the complexity of sex ratio adjustment in birds. In spite of our negative results, the lack of an association between brood sex ratio and parental quality and environmental factors in the Yellow-bellied Prinia provides valuable information on the adjustment of sex ratios in birds. | Zhifeng Ding Fang Ji Qiuli Huang Longwu Wang Aiwu Jiang Chunlan Zhang Yongjun Feng Yuan Tian Huijian Hu Wei Liang | 2017 | Avian Research2017，8，2： | 2 |
| 4 | Copper and Manganese Induce Yeast Apoptosis via Different Pathways显示文摘 | Qiuli Liang Bing Zhou | 2007 | Molecular Biology of the Cell2007，18，12： | 1 |
| 5 | Copper and Manganese Induce Yeast Apoptosis via Different Pathways 显示文摘 | Qiuli Liang Bing Zhou | 2007 | Molecular Biology of the Ce112007，18，12： | 1 |
| 6 | Long noncoding RNA LINC01088 inhibits esophageal squamous cell carcinoma progression by targeting the NPM1-HDM2-p53 axis显示文摘Esophageal squamous cell carcinoma(ESCC)is characterized by extensive metastasis and poor prognosis.Long noncoding RNAs(lncRNAs)have been shown to play important roles in ESCC.However,the specific roles of lncRNAs in ESCC tumorigenesis and metastasis remain largely unknown.Here,we investigate LINC01088 in ESCC.Differentially expressed LINC01088 levels are screened from the GEO database.We find that LINC01088 is expressed at low level in collected clinical samples and is correlated with vascular tumor emboli and poor overall survival time of patients after surgery.LINC01088 inhibits not only ESCC cell migration and invasion in vitro,but also tumorigenesis and metastasis in vivo.Mechanistically,LINC01088 directly interacts with nucleophosmin(NPM1)and increases the expression of NPM1 in the nucleoplasm compared to that in the nucleolar region.LINC01088 decreases mutant p53(mut-p53)expression and rescues the transcriptional activity of p53 by targeting the NPM1-HDM2-p53 axis.LINC01088 may also interfere with the DNA repair function of NPM1 by affecting its translocation.Our results highlight the potential of LINC01088 as a prognostic biomarker and therapeutic target of ESCC. | Fan Liang Qiuli Luo Haibo Han Jianzhi Zhang Yue Yang Jinfeng Chen | 2023 | Acta Biochimica et Biophysica Sinica2023，55，3： | 0 |
| 7 | Comparison of Anti-inflammatory and Analgesic Effects of Fresh and Dry Products of Zhuang Medicine Asystaia gangetica显示文摘[Objectives]To compare anti-inflammatory and analgesic effects of fresh and dry products of Zhuang medicine Asystaia gangetica on mice.[Methods]Experiments were performed using glacial acetic acid to increase capillary permeability in mice,cotton ball to cause granulomatous formation in mice,xylene to cause auricular swelling in mice,chronic inflammation,and acetic acid-induced pain and hot-plate pain.Besides,the anti-inflammatory and analgesic effects of fresh and dry products of A.gangetica on mice were observed with the indicators of capillary permeability in the abdominal cavity,swelling rate of auricles,cotton ball granulomas and writhing times,and thermal pain threshold.[Results]Fresh products of A.gangetica had significant inhibitory effects on peritoneal capillary permeability increase in mice induced by glacial acetic acid,mouse auricle swelling induced by xylene,and mouse granuloma formation induced by cotton balls.Dry products of A.gangetica also significantly inhibited the auricle swelling in mice induced by xylene,but the effect intensity was weaker than that of fresh products.In addition,dry products of A.gangetica showed a certain tendency to inhibit the increase of capillary permeability in the abdominal cavity of mice induced by glacial acetic acid.In the analgesic experiment,both fresh and dry products of A.gangetica showed better analgesic effect,but caused pain on the hot plate,and the analgesic effect time of fresh products was shorter than that of dry products.[Conclusions]Both fresh and dry products of Zhuang medicine A.gangetica have certain anti-inflammatory and analgesic effects. | Ke YANG Xianxing LIANG Ning XU Qiuli LONG Fang CHEN Chunhui ZENG | 2020 | Medicinal Plant2020，11，3： | 0 |
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