| 1 | Nitrogen(N) metabolism related enzyme activities,cell uitrastructure and nutrient contents as affected by N level and barley genotype显示文摘Development of the new crop cultivars with high yield under low nitrogen(N) input is a fundamental approach to enhance agricultural sustainability,which is dependent on the exploitation of the elite germplasm.In the present study,four barley genotypes(two Tibetan wild and two cultivated),differing in N use efficiency(NUE),were characterized for their physiological and biochemical responses to different N levels.Higher N levels significantly increased the contents of other essential nutrients(P,K,Ca,Fe,Cu and Mn),and the increase was more obvious for the N-efficient genotypes(ZD9 and XZ149).The observation of ultrastructure showed that chloroplast structure was severely damaged under low nitrogen,and the two high N efficient genotypes were relatively less affected.The activities of the five N metabolism related enzymes,i.e.,nitrate reductase(NR),glutamine synthetase(GS),nitrite reductase(NiR),glutamate synthase(GOGAT) and glutamate dehydrogenase(GDH) all showed the substantial increase with the increased N level in the culture medium.However the increased extent differed among the four genotypes,with the two N efficient genotypes showing more increase in comparison with the other two genotypes with relative N inefficiency(HXRL and XZ56).The current findings showed that a huge difference exists in low N tolerance among barley genotypes,and improvement of some physiological traits(such as enzymes) could be helpful for increasing N utilization efficiency. | Jawad Munawar Shah Syed Asad Hussain Bukhari ZENG Jian-bin QUAN Xiao-yan Essa Ali Noor Muhammad ZHANG Guo-ping | 2017 | Journal of Integrative Agriculture2017，16，1： | 4 |
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