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| 1 | Magnetic bioassembly platforms towards the generation of extracellular vesicles from human salivary gland functional organoids for epithelial repair显示文摘Salivary glands(SG)are exocrine organs with secretory units commonly injured by radiotherapy.Bio-engineered organoids and extracellular vesicles(EV)are currently under investigation as potential strategies for SG repair.Herein,three-dimensional(3D)cultures of SG functional organoids(SGo)and human dental pulp stem cells(hDPSC)were generated by magnetic 3D bioassembly(M3DB)platforms.Fibroblast growth factor 10(FGF10)was used to enrich the SGo in secretory epithelial units.After 11 culture days via M3DB,SGo displayed SG-specific acinar epithelial units with functional properties upon neurostimulation.To consistently develop 3D hDPSC in vitro,3 culture days were sufficient to maintain hDPSC undifferentiated genotype and phenotype for EV generation.EV isolation was performed via sequential centrifugation of the conditioned media of hDPSC and SGo cultures.EV were characterized by nanoparticle tracking analysis,electron microscopy and immunoblotting.EV were in the exosome range for hDPSC(diameter:88.03±15.60 nm)and for SGo(123.15±63.06 nm).Upon ex vivo administration,exosomes derived from SGo significantly stimulated epithelial growth(up to 60%),mitosis,epithelial progenitors and neuronal growth in injured SG;however,such biological effects were less distinctive with the ones derived from hDPSC.Next,these exosome biological effects were investigated by proteomic arrays.Mass spectrometry profiling of SGo exosomes predicted that cellular growth,development and signaling was due to known and undocumented molecular targets downstream of FGF10.Semaphorins were identified as one of the novel targets requiring further investigations.Thus,M3DB platforms can generate exosomes with potential to ameliorate SG epithelial damage. | Ajjima Chansaenroj Christabella Adine Sawanya Charoenlappanit Sittiruk Roytrakul Ladawan Sariya Thanaphum Osathanon Sasitorn Rungarunlert Ganokon Urkasemsin Risa Chaisuparat Supansa Yodmuang Glauco R.Souza Joao N.Ferreira | 2022 | Bioactive Materials2022，7，12： | 1 |
| 2 | Hydrogen-based direct reduction of iron oxide at 700℃:Heterogeneity at pellet and microstructure scales显示文摘Steel production causes a third of all industrial CO_(2) emissions due to the use of carbon-based substances as reductants for iron ores,making it a key driver of global warming.Therefore,research efforts aim to replace these reductants with sustainably produced hydrogen.Hydrogen-based direct reduction(HyDR)is an attractive processing technology,given that direct reduction(DR)furnaces are routinely operated in the steel industry but with CH_(4) or CO as reductants.Hydrogen diffuses considerably faster through shaft-furnace pellet agglomerates than carbon-based reductants.However,the net reduction kinetics in HyDR remains extremely sluggish for high-quantity steel production,and the hydrogen consumption exceeds the stoichiometrically required amount substantially.Thus,the present study focused on the improved understanding of the influence of spatial gradients,morphology,and internal microstructures of ore pellets on reduction efficiency and metallization during HyDR.For this purpose,commercial DR pellets were investigated using synchrotron high-energy X-ray diffraction and electron microscopy in conjunction with electron backscatter diffraction and chemical probing.Revealing the interplay of different phases with internal interfaces,free surfaces,and associated nucleation and growth mechanisms provides a basis for developing tailored ore pellets that are highly suited for a fast and efficient HyDR. | Yan Ma Isnaldi R.Souza Filho Xue Zhang Supriya Nandy Pere Barriobero-Vila Guillermo Requena Dirk Vogel Michael Rohwerder Dirk Ponge Hauke Springer Dierk Raabe | 2022 | International Journal of Minerals,Metallurgy and Materials2022，29，10： | 1 |
| 3 | 以髋关节镜检查为标准验证髋关节炎MRI评分(SHOMRI)的准确性显示文摘目的基于髋关节镜对髋关节异常的评估来验证术前髋关节MRI检查进行髋关节炎MRI评分(SHOMRI)分级的准确性。方法2名放射科医师回顾性分析了39例股骨髋臼撞击综合征病人[平均年龄(34.7±9.0)岁。 | J.Neumann A.L.Zhang B.J.Schwaiger M.A.Samaan R.Souza S.C.Foreman 宋燕燕(译) 赵世华(校) | 2019 | 国际医学放射学杂志2019，0，2： | 1 |
| 4 | Linking the spatiotemporal variation of litterfall to standing vegetation biomass in Brazilian savannas显示文摘全球尺度上的凋落物受气候,土壤特征和植被结构的影响,随着植被现存生物量的增加,从草地到森林的凋落物产量增加。然而,在景观尺度上,凋落物的产量与植被结构之间的相同关系却很少被研究,相邻的结构不同的群落之间凋落物格局的比较研究也比较缺乏。本研究使用一种标准化的方法来描述四种稀树草原地貌之间的结构差异,并分析了在巴西塞拉多保护区(Cerrado)中地貌与凋落物变化的关系。我们评估了平均分布在4个地貌的48个地点的木本植被结构和组成,并从2014年4月到2015年3月每月监测凋落物产量。研究结果表明,不同地貌的木本植被密度、断面积、柱状体积和地上生物量各不相同。地上生物量与年凋落物产量之间存在着很强的正相关关系,凋落物产量随植被结构的增加从0.9增加到8.4 Mg ha^(−1)。不同植被类型间的月产量具有季节性和相似性,旱季产量增加。叶组成显性部分(约85%)和凋落物的季节性主要是由于干旱月份落叶集中所致。然而,可能是随着植物群落中落叶物种丰度的减少,全年凋落物的时间格局显示出从开放植被类型到封闭植被类型的季节性逐渐减少。我们的研究结果表明,植被结构的变化可能会影响不同地貌凋落物的空间和时间分布格局,这些格局在整个塞拉多保护区景观中同时发生,可能对该生态系统的整体功能产生影响。此外,这些发现强调了使用标准化方法是正确比较不同环境中凋落物格局的必要条件。 | Alan N.Costa Jeane R.Souza Karyne M.Alves Anderson Penna-Oliveira Geisciele Paula-Silva Ingrid S.Becker Kelly Marinho-Vieira Ana L.Bonfim Alessandra Bartimachi Ernane H.M.Vieira-Neto | 2020 | Journal of Plant Ecology2020，13，5： | 0 |
| 5 | 生物技术在制革中的应用显示文摘在浸水和浸灰工序进行了使用化工材料和酶辅助的试验,以及只使用化工材料的传统试验。通过对皮中氯化物和脂肪的去除进行分析以及对SEM图像分析,得出:在浸水工序中,当变量为时间时,酶辅助试验的效果较好,而且,在脱毛工序中,酶的使用对脱毛更有效。在其他研究中,试验表明:利用酶的作用从皮革废弃物中去除铬,可以减少53.7%的残渣(质量分数)。使用绿脓假单胞菌进行废弃物处理可以减少初始铬含量的57.4%。所以说,生物技术可以在皮革生产中使用并有利于减少其对土壤和水的污染。 | Mariliz Gutterres Aline Dettmer Luciani A Amaral Franck R.Souza Marcelo F de Sousa 庞晓燕 | 2009 | 中国皮革2009，38，23： | 0 |
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